##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AvrII BamHI BbeI BcgI BciVI
BclI BfrI BlnI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HindIII
HpaI KasI KpnI KspI MluI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SspBI SspI
SwaI TaqI TthHB8I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 1594 GTAGAC GT^[AC][GT]AC
AccIII 353 TCCGGA T^CCGGA
AcyI 2081 GACGCC G[GA]^CG[CT]C
AhdI 832 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 927 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 832 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2464 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 188 CCCGGG C^[CT]CG[GA]G
AviII 1181 TGCGCA TGC^GCA
BbrPI 202 CACGTG CAC^GTG
BlpI 264 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 264 GCTAAGC GC^T[ACGT]AGC
BsaAI 202 CACGTG [CT]AC^GT[GA]
BsaHI 2081 GACGCC G[GA]^CG[CT]C
BsaWI 353 TCCGGA [AT]^CCGG[AT]
BseAI 353 TCCGGA T^CCGGA
BsoBI 188 CCCGGG C^[CT]CG[GA]G
BspEI 353 TCCGGA T^CCGGA
BspHI 2399 TCATGA T^CATGA
BsrDI 1939 CATTGC GCAATG(2/0)
BsrFI 1979 GCCGGC [GA]^CCGG[CT]
BssHII 2326 GCGCGC G^CGCGC
BssSI 1303 CTCGTG CACGAG(-5/-1)
CelII 264 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1979 GCCGGC [GA]^CCGG[CT]
DraIII 70 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1060 GACTTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 832 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 891 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2047 CCAAGG C^C[AT][AT]GG
FspI 1181 TGCGCA TGC^GCA
Hin1I 2081 GACGCC G[GA]^CG[CT]C
HincII 61 GTCAAC GT[CT]^[GA]AC
HindII 61 GTCAAC GT[CT]^[GA]AC
MfeI 1533 CAATTG C^AATTG
MroI 353 TCCGGA T^CCGGA
MseI 2474 TTAA T^TAA
MunI 1533 CAATTG C^AATTG
NaeI 1979 GCCGGC GCC^GGC
NgoMIV 1979 GCCGGC G^CCGGC
NspI 344 GCATGC [GA]CATG^[CT]
PflFI 2464 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 202 CACGTG CAC^GTG
PmlI 202 CACGTG CAC^GTG
RcaI 2399 TCATGA T^CATGA
SacI 655 GAGCTC GAGCT^C
SbfI 1729 CCTGCAGG CCTGCA^GG
ScaI 1237 AGTACT AGT^ACT
SmaI 188 CCCGGG CCC^GGG
SphI 344 GCATGC GCATG^C
Sse8387I 1729 CCTGCAGG CCTGCA^GG
StuI 2360 AGGCCT AGG^CCT
StyI 2047 CCAAGG C^C[AT][AT]GG
TfiI 2103 GATTC G^A[AT]TC
Tru9I 2474 TTAA T^TAA
Tth111I 2464 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1503 TCTAGA T^CTAGA
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
XmnI 927 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 369 CGCG CG^CG
2327 CGCG
Alw44I 67 GTGCAC G^TGCAC
1351 GTGCAC
ApaLI 67 GTGCAC G^TGCAC
1351 GTGCAC
BanI 208 GGTGCC G^G[CT][GA]CC
1427 GGTGCC
BbsI 1113 GAAGAC GAAGAC(2/6)
45 GTCTTC
BbvCI 103 CCTCAGC CCTCAGC(-5/-2)
1003 GCTGAGG
BglI 1943 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2063 GCCATCAAGGC
BglII 2115 AGATCT A^GATCT
2429 AGATCT
BpuAI 1113 GAAGAC GAAGAC(2/6)
45 GTCTTC
BsaBI 1087 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1705 GATGCCGATC
BsaI 584 GAGACC GGTCTC(1/5)
2008 GAGACC
BsgI 323 CTGCAC GTGCAG(16/14)
1030 CTGCAC
BspMI 1178 ACCTGC ACCTGC(4/8)
91 GCAGGT
BsrBI 1447 CCGCTC CCGCTC(-3/-3)
217 GAGCGG
BstUI 369 CGCG CG^CG
2327 CGCG
BstYI 2115 AGATCT [GA]^GATC[CT]
2429 AGATCT
DraII 93 AGGTCCC [GA]G^G[ACGT]CC[CT]
1516 GGGACCT
EcoO109I 93 AGGTCCC [GA]G^G[ACGT]CC[CT]
1516 GGGACCT
HgaI 751 GACGC GACGC(5/10)
2081 GACGC
MamI 1087 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1705 GATGCCGATC
MflI 2115 AGATCT [GA]^GATC[CT]
2429 AGATCT
MvnI 369 CGCG CG^CG
2327 CGCG
PpuMI 93 AGGTCCC [GA]G^G[AT]CC[CT]
1516 GGGACCT
PstI 1129 CTGCAG CTGCA^G
1730 CTGCAG
SexAI 71 ACCTGGT A^CC[AT]GGT
333 ACCTGGT
XcmI 918 CCATAACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1938 CCATTGCCTCTCTGG
XhoII 2115 AGATCT [GA]^GATC[CT]
2429 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I Tsp509I
TspRI