##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AccI AccIII AclI AcsI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BglI
BglII BlnI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp45I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1068 GACGTC GACGT^C
Acc65I 1416 GGTACC G^GTACC
AflII 1330 CTTAAG C^TTAAG
Asp718I 1416 GGTACC G^GTACC
AspI 394 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1619 TGGCCA TGG^CCA
BfaI 1225 CTAG C^TAG
BfrI 1330 CTTAAG C^TTAAG
BsaAI 1024 TACGTG [CT]AC^GT[GA]
BsaWI 1704 ACCGGA [AT]^CCGG[AT]
BsrFI 1345 GCCGGC [GA]^CCGG[CT]
BstAPI 466 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1508 TTCGAA TT^CGAA
Cfr10I 1345 GCCGGC [GA]^CCGG[CT]
FauI 279 GCGGG CCCGC(4/6)
HgaI 1007 GACGC GACGC(5/10)
KpnI 1416 GGTACC GGTAC^C
MaeI 1225 CTAG C^TAG
MaeIII 1383 GTTAC ^GT[ACGT]AC
MluNI 1619 TGGCCA TGG^CCA
MscI 1619 TGGCCA TGG^CCA
MseI 1331 TTAA T^TAA
NaeI 1345 GCCGGC GCC^GGC
NgoMIV 1345 GCCGGC G^CCGGC
NspI 809 GCATGC [GA]CATG^[CT]
NspV 1508 TTCGAA TT^CGAA
PflFI 394 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1313 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1267 CTGCAG CTGCA^G
SacI 1591 GAGCTC GAGCT^C
SexAI 1784 ACCTGGT A^CC[AT]GGT
SfcI 1267 CTGCAG C^T[GA][CT]AG
SfuI 1508 TTCGAA TT^CGAA
SmaI 783 CCCGGG CCC^GGG
SmlI 1330 CTTAAG C^T[CT][GA]AG
SphI 809 GCATGC GCATG^C
StuI 1580 AGGCCT AGG^CCT
TaqI 1509 TCGA T^CGA
Tru9I 1331 TTAA T^TAA
Tsp509I 844 AATT ^AATT
Tth111I 394 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1509 TCGA T^CGA
XcmI 1622 CCAGTCGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 783 CCCGGG C^CCGGG
XmaI 783 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 356 CGCG CG^CG
1150 CGCG
AcyI 1007 GACGCC G[GA]^CG[CT]C
1068 GACGTC
ApaI 134 GGGCCC GGGCC^C
1613 GGGCCC
AspHI 922 GTGCTC G[AT]GC[AT]^C
1591 GAGCTC
AvaI 783 CCCGGG C^[CT]CG[GA]G
1274 CTCGGG
BamHI 693 GGATCC G^GATCC
1432 GGATCC
BbsI 307 GAAGAC GAAGAC(2/6)
1 GTCTTC
BpuAI 307 GAAGAC GAAGAC(2/6)
1 GTCTTC
BsaHI 1007 GACGCC G[GA]^CG[CT]C
1068 GACGTC
BsaI 550 GAGACC GGTCTC(1/5)
513 GGTCTC
BsgI 664 GTGCAG GTGCAG(16/14)
904 GTGCAG
BsiHKAI 922 GTGCTC G[AT]GC[AT]^C
1591 GAGCTC
BsoBI 783 CCCGGG C^[CT]CG[GA]G
1274 CTCGGG
BstF5I 1691 GGATG GGATG(2/0)
1758 GGATG
BstUI 356 CGCG CG^CG
1150 CGCG
BstXI 518 CCATAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
755 CCAAGATCCTGG
FokI 1691 GGATG GGATG(9/13)
1758 GGATG
Hin1I 1007 GACGCC G[GA]^CG[CT]C
1068 GACGTC
MaeII 1025 ACGT A^CGT
1069 ACGT
MvnI 356 CGCG CG^CG
1150 CGCG
NdeI 66 CATATG CA^TATG
578 CATATG
SapI 423 GAAGAGC GCTCTTC(1/4)
924 GCTCTTC
SfaNI 96 GATGC GCATC(5/9)
250 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvCI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI StyI TfiI
TseI TspRI Van91I XhoII