##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BcgI BciVI BclI BfrI BglI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1226 CGCG CG^CG
AflIII 709 ACATGT A^C[GA][CT]GT
AhdI 599 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 568 CCTGAGG CC^T[ACGT]AGG
Asp700I 1072 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 599 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 714 TGGCCA TGG^CCA
BbrPI 626 CACGTG CAC^GTG
BglII 1454 AGATCT A^GATCT
BlpI 1244 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1244 GCTCAGC GC^T[ACGT]AGC
BsaAI 626 CACGTG [CT]AC^GT[GA]
BspLU11I 709 ACATGT A^CATGT
BsrBI 253 CCGCTC CCGCTC(-3/-3)
BssHII 1225 GCGCGC G^CGCGC
BstUI 1226 CGCG CG^CG
BstYI 1454 AGATCT [GA]^GATC[CT]
Bsu36I 568 CCTGAGG CC^T[ACGT]AGG
CelII 1244 GCTCAGC GC^T[ACGT]AGC
DraIII 1121 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 85 CCATGG C^C[GA][CT]GG
Eam1105I 599 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 568 CCTGAGG CC^T[ACGT]AGG
EcoNI 536 CCTCGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 754 GCGTC GACGC(5/10)
MaeII 627 ACGT A^CGT
MflI 1454 AGATCT [GA]^GATC[CT]
MluNI 714 TGGCCA TGG^CCA
MscI 714 TGGCCA TGG^CCA
MslI 1123 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 304 CCGCTG C[AC]G^C[GT]G
MvnI 1226 CGCG CG^CG
NcoI 85 CCATGG C^CATGG
PmaCI 626 CACGTG CAC^GTG
PmlI 626 CACGTG CAC^GTG
PshAI 540 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1145 GAGCTC GAGCT^C
ScaI 1191 AGTACT AGT^ACT
SfaNI 17 GATGC GCATC(5/9)
SmaI 354 CCCGGG CCC^GGG
SmlI 65 CTCAAG C^T[CT][GA]AG
TaqI 538 TCGA T^CGA
TthHB8I 538 TCGA T^CGA
XhoII 1454 AGATCT [GA]^GATC[CT]
XmaCI 354 CCCGGG C^CCGGG
XmaI 354 CCCGGG C^CCGGG
XmnI 1072 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 412 AAATTT [GA]^AATT[CT]
1280 GAATTT
AcyI 154 GGCGCC G[GA]^CG[CT]C
942 GGCGCC
ApoI 412 AAATTT [GA]^AATT[CT]
1280 GAATTT
AspHI 1145 GAGCTC G[AT]GC[AT]^C
1242 GTGCTC
BbeI 154 GGCGCC GGCGC^C
942 GGCGCC
BbvCI 175 CCTCAGC CCTCAGC(-5/-2)
798 CCTCAGC
BfaI 530 CTAG C^TAG
1031 CTAG
BsaHI 154 GGCGCC G[GA]^CG[CT]C
942 GGCGCC
BsaI 239 GAGACC GGTCTC(1/5)
1109 GAGACC
BsiHKAI 1145 GAGCTC G[AT]GC[AT]^C
1242 GTGCTC
BstF5I 110 CATCC GGATG(2/0)
786 CATCC
BstXI 41 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
907 CCAAGGAGATGG
EaeI 301 TGGCCG [CT]^GGCC[GA]
714 TGGCCA
FauI 891 CCCGC CCCGC(4/6)
162 GCGGG
FokI 110 CATCC GGATG(9/13)
786 CATCC
Hin1I 154 GGCGCC G[GA]^CG[CT]C
942 GGCGCC
HphI 268 GGTGA GGTGA(8/7)
203 TCACC
KasI 154 GGCGCC G^GCGCC
942 GGCGCC
MaeI 530 CTAG C^TAG
1031 CTAG
MaeIII 202 GTCAC ^GT[ACGT]AC
624 GTCAC
NarI 154 GGCGCC GG^CGCC
942 GGCGCC
NspI 33 ACATGC [GA]CATG^[CT]
709 ACATGT
SfoI 154 GGCGCC GGC^GCC
942 GGCGCC
Tsp45I 202 GTCAC ^GT[GC]AC
624 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI StyI Tru9I TseI Tsp509I
TspRI