##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AviII BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlpI Bpu1102I BsaAI BsaBI
BseRI BsiEI BsiWI BsmI Bsp1407I
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HgaI
HindIII KpnI KspI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccII 271 CGCG CG^CG
AccIII 392 TCCGGA T^CCGGA
AcyI 137 GGCGCC G[GA]^CG[CT]C
AflIII 1721 ACGTGT A^C[GA][CT]GT
AhdI 1734 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1622 GTGCAC G^TGCAC
AocI 1698 CCTCAGG CC^T[ACGT]AGG
ApaLI 1622 GTGCAC G^TGCAC
AspEI 1734 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1582 CCCGGG C^[CT]CG[GA]G
BalI 1593 TGGCCA TGG^CCA
BamHI 952 GGATCC G^GATCC
BanI 137 GGCGCC G^G[CT][GA]CC
BbeI 137 GGCGCC GGCGC^C
BbsI 383 GTCTTC GAAGAC(2/6)
BglII 1139 AGATCT A^GATCT
BpuAI 383 GTCTTC GAAGAC(2/6)
BsaHI 137 GGCGCC G[GA]^CG[CT]C
BsaWI 392 TCCGGA [AT]^CCGG[AT]
BseAI 392 TCCGGA T^CCGGA
BsmBI 561 GAGACG CGTCTC(1/5)
BsoBI 1582 CCCGGG C^[CT]CG[GA]G
BspDI 828 ATCGAT AT^CGAT
BspEI 392 TCCGGA T^CCGGA
BspHI 611 TCATGA T^CATGA
BsrDI 938 CATTGC GCAATG(2/0)
BstAPI 1032 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 271 CGCG CG^CG
Bsu36I 1698 CCTCAGG CC^T[ACGT]AGG
CfoI 138 GCGC GCG^C
ClaI 828 ATCGAT AT^CGAT
Eam1105I 1734 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1698 CCTCAGG CC^T[ACGT]AGG
HaeII 137 GGCGCC [GA]GCGC^[CT]
HhaI 138 GCGC GCG^C
Hin1I 137 GGCGCC G[GA]^CG[CT]C
HinP1I 138 GCGC G^CGC
HincII 368 GTTAAC GT[CT]^[GA]AC
HindII 368 GTTAAC GT[CT]^[GA]AC
HpaI 368 GTTAAC GTT^AAC
KasI 137 GGCGCC G^GCGCC
MluNI 1593 TGGCCA TGG^CCA
MroI 392 TCCGGA T^CCGGA
MscI 1593 TGGCCA TGG^CCA
MspA1I 699 CAGCTG C[AC]G^C[GT]G
MvnI 271 CGCG CG^CG
NarI 137 GGCGCC GG^CGCC
NcoI 910 CCATGG C^CATGG
NruI 270 TCGCGA TCG^CGA
NspI 1501 GCATGT [GA]CATG^[CT]
PflMI 337 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1585 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 207 CTGCAG CTGCA^G
PvuII 699 CAGCTG CAG^CTG
RcaI 611 TCATGA T^CATGA
SacI 1365 GAGCTC GAGCT^C
ScaI 736 AGTACT AGT^ACT
SfoI 137 GGCGCC GGC^GCC
SmaI 1582 CCCGGG CCC^GGG
TaqI 829 TCGA T^CGA
TthHB8I 829 TCGA T^CGA
Van91I 337 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1582 CCCGGG C^CCGGG
XmaI 1582 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 218 AAATTT [GA]^AATT[CT]
440 AAATTC
AlwNI 51 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1701 CAGGCTCTG
ApaI 1145 GGGCCC GGGCC^C
1579 GGGCCC
ApoI 218 AAATTT [GA]^AATT[CT]
440 AAATTC
AspHI 1365 GAGCTC G[AT]GC[AT]^C
1622 GTGCAC
AvrII 1110 CCTAGG C^CTAGG
1209 CCTAGG
BbvI 357 GCAGC GCAGC(8/12)
819 GCAGC
BlnI 1110 CCTAGG C^CTAGG
1209 CCTAGG
BpmI 492 CTGGAG CTGGAG(16/14)
685 CTGGAG
BsgI 817 GTGCAG GTGCAG(16/14)
1039 GTGCAG
BsiHKAI 1365 GAGCTC G[AT]GC[AT]^C
1622 GTGCAC
BstYI 952 GGATCC [GA]^GATC[CT]
1139 AGATCT
DsaI 622 CCGTGG C^C[GA][CT]GG
910 CCATGG
EaeI 676 TGGCCG [CT]^GGCC[GA]
1593 TGGCCA
EcoNI 1110 CCTAGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1204 CCTGACCTAGG
EcoRV 157 GATATC GAT^ATC
1096 GATATC
FauI 850 CCCGC CCCGC(4/6)
101 GCGGG
Fnu4HI 357 GCAGC GC^[ACGT]GC
819 GCAGC
ItaI 357 GCAGC GC^[ACGT]GC
819 GCAGC
MaeII 381 ACGT A^CGT
1721 ACGT
MflI 952 GGATCC [GA]^GATC[CT]
1139 AGATCT
SapI 426 GAAGAGC GCTCTTC(1/4)
1689 GAAGAGC
SmlI 570 CTCAAG C^T[CT][GA]AG
669 CTCAAG
StuI 26 AGGCCT AGG^CCT
1409 AGGCCT
TfiI 194 GATTC G^A[AT]TC
401 GATTC
TseI 357 GCAGC G^C[AT]GC
819 GCAGC
XcmI 337 CCAAGGGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
362 CCACAAGTTAACTGG
XhoII 952 GGATCC [GA]^GATC[CT]
1139 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BcnI BfaI BmyI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FokI
HaeIII HapII HinfI HpaII HphI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I Tsp45I
Tsp509I TspRI