################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BseRI BsiEI BsiWI BsmI Bsp1407I BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HgaI HindIII KpnI KspI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccII 271 CGCG CG^CG AccIII 392 TCCGGA T^CCGGA AcyI 137 GGCGCC G[GA]^CG[CT]C AflIII 1721 ACGTGT A^C[GA][CT]GT AhdI 1734 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1622 GTGCAC G^TGCAC AocI 1698 CCTCAGG CC^T[ACGT]AGG ApaLI 1622 GTGCAC G^TGCAC AspEI 1734 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1582 CCCGGG C^[CT]CG[GA]G BalI 1593 TGGCCA TGG^CCA BamHI 952 GGATCC G^GATCC BanI 137 GGCGCC G^G[CT][GA]CC BbeI 137 GGCGCC GGCGC^C BbsI 383 GTCTTC GAAGAC(2/6) BglII 1139 AGATCT A^GATCT BpuAI 383 GTCTTC GAAGAC(2/6) BsaHI 137 GGCGCC G[GA]^CG[CT]C BsaWI 392 TCCGGA [AT]^CCGG[AT] BseAI 392 TCCGGA T^CCGGA BsmBI 561 GAGACG CGTCTC(1/5) BsoBI 1582 CCCGGG C^[CT]CG[GA]G BspDI 828 ATCGAT AT^CGAT BspEI 392 TCCGGA T^CCGGA BspHI 611 TCATGA T^CATGA BsrDI 938 CATTGC GCAATG(2/0) BstAPI 1032 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 271 CGCG CG^CG Bsu36I 1698 CCTCAGG CC^T[ACGT]AGG CfoI 138 GCGC GCG^C ClaI 828 ATCGAT AT^CGAT Eam1105I 1734 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1698 CCTCAGG CC^T[ACGT]AGG HaeII 137 GGCGCC [GA]GCGC^[CT] HhaI 138 GCGC GCG^C Hin1I 137 GGCGCC G[GA]^CG[CT]C HinP1I 138 GCGC G^CGC HincII 368 GTTAAC GT[CT]^[GA]AC HindII 368 GTTAAC GT[CT]^[GA]AC HpaI 368 GTTAAC GTT^AAC KasI 137 GGCGCC G^GCGCC MluNI 1593 TGGCCA TGG^CCA MroI 392 TCCGGA T^CCGGA MscI 1593 TGGCCA TGG^CCA MspA1I 699 CAGCTG C[AC]G^C[GT]G MvnI 271 CGCG CG^CG NarI 137 GGCGCC GG^CGCC NcoI 910 CCATGG C^CATGG NruI 270 TCGCGA TCG^CGA NspI 1501 GCATGT [GA]CATG^[CT] PflMI 337 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1585 GGGTCCT [GA]G^G[AT]CC[CT] PstI 207 CTGCAG CTGCA^G PvuII 699 CAGCTG CAG^CTG RcaI 611 TCATGA T^CATGA SacI 1365 GAGCTC GAGCT^C ScaI 736 AGTACT AGT^ACT SfoI 137 GGCGCC GGC^GCC SmaI 1582 CCCGGG CCC^GGG TaqI 829 TCGA T^CGA TthHB8I 829 TCGA T^CGA Van91I 337 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1582 CCCGGG C^CCGGG XmaI 1582 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 218 AAATTT [GA]^AATT[CT] 440 AAATTC AlwNI 51 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 1701 CAGGCTCTG ApaI 1145 GGGCCC GGGCC^C 1579 GGGCCC ApoI 218 AAATTT [GA]^AATT[CT] 440 AAATTC AspHI 1365 GAGCTC G[AT]GC[AT]^C 1622 GTGCAC AvrII 1110 CCTAGG C^CTAGG 1209 CCTAGG BbvI 357 GCAGC GCAGC(8/12) 819 GCAGC BlnI 1110 CCTAGG C^CTAGG 1209 CCTAGG BpmI 492 CTGGAG CTGGAG(16/14) 685 CTGGAG BsgI 817 GTGCAG GTGCAG(16/14) 1039 GTGCAG BsiHKAI 1365 GAGCTC G[AT]GC[AT]^C 1622 GTGCAC BstYI 952 GGATCC [GA]^GATC[CT] 1139 AGATCT DsaI 622 CCGTGG C^C[GA][CT]GG 910 CCATGG EaeI 676 TGGCCG [CT]^GGCC[GA] 1593 TGGCCA EcoNI 1110 CCTAGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1204 CCTGACCTAGG EcoRV 157 GATATC GAT^ATC 1096 GATATC FauI 850 CCCGC CCCGC(4/6) 101 GCGGG Fnu4HI 357 GCAGC GC^[ACGT]GC 819 GCAGC ItaI 357 GCAGC GC^[ACGT]GC 819 GCAGC MaeII 381 ACGT A^CGT 1721 ACGT MflI 952 GGATCC [GA]^GATC[CT] 1139 AGATCT SapI 426 GAAGAGC GCTCTTC(1/4) 1689 GAAGAGC SmlI 570 CTCAAG C^T[CT][GA]AG 669 CTCAAG StuI 26 AGGCCT AGG^CCT 1409 AGGCCT TfiI 194 GATTC G^A[AT]TC 401 GATTC TseI 357 GCAGC G^C[AT]GC 819 GCAGC XcmI 337 CCAAGGGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 362 CCACAAGTTAACTGG XhoII 952 GGATCC [GA]^GATC[CT] 1139 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BcnI BfaI BmyI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FokI HaeIII HapII HinfI HpaII HphI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I Tsp45I Tsp509I TspRI