##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BseAI BseRI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HpaI KpnI MamI MfeI MluI
MroI MslI MunI NcoI NdeI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccII 887 CGCG CG^CG
AcyI 1058 GGCGCC G[GA]^CG[CT]C
AfaI 767 GTAC GT^AC
AlwI 693 GATCC GGATC(4/5)
AlwNI 932 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1011 GAGCAC G[AT]GC[AT]^C
AspI 621 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 407 GGACC G^G[AT]CC
BalI 758 TGGCCA TGG^CCA
BbeI 1058 GGCGCC GGCGC^C
BcgI 1013 GCACTTCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 527 GCTCAGC GC^T[ACGT]AGC
BpmI 300 CTGGAG CTGGAG(16/14)
Bpu1102I 527 GCTCAGC GC^T[ACGT]AGC
BsaHI 1058 GGCGCC G[GA]^CG[CT]C
BsaWI 964 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1011 GAGCAC G[AT]GC[AT]^C
BsmBI 626 CGTCTC CGTCTC(1/5)
BspMI 832 GCAGGT ACCTGC(4/8)
BstAPI 1287 GCATGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 887 CGCG CG^CG
BstXI 781 CCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 692 AGATCC [GA]^GATC[CT]
CelII 527 GCTCAGC GC^T[ACGT]AGC
DsaI 886 CCGCGG C^C[GA][CT]GG
EaeI 758 TGGCCA [CT]^GGCC[GA]
EarI 1008 GAAGAG CTCTTC(1/4)
EcoNI 1177 CCTCACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1144 CCAAGG C^C[AT][AT]GG
HaeII 1058 GGCGCC [GA]GCGC^[CT]
Hin1I 1058 GGCGCC G[GA]^CG[CT]C
HincII 672 GTCAAC GT[CT]^[GA]AC
HindII 672 GTCAAC GT[CT]^[GA]AC
HindIII 475 AAGCTT A^AGCTT
KasI 1058 GGCGCC G^GCGCC
Ksp632I 1008 GAAGAG CTCTTC(1/4)
KspI 886 CCGCGG CCGC^GG
MaeII 625 ACGT A^CGT
MflI 692 AGATCC [GA]^GATC[CT]
MluNI 758 TGGCCA TGG^CCA
MscI 758 TGGCCA TGG^CCA
MvnI 887 CGCG CG^CG
NaeI 1055 GCCGGC GCC^GGC
NarI 1058 GGCGCC GG^CGCC
NgoMIV 1055 GCCGGC G^CCGGC
NheI 373 GCTAGC G^CTAGC
PaeR7I 101 CTCGAG C^TCGAG
PflFI 621 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 422 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 730 CTGCAG CTGCA^G
PvuII 838 CAGCTG CAG^CTG
RsaI 767 GTAC GT^AC
SacII 886 CCGCGG CCGC^GG
SapI 1008 GAAGAGC GCTCTTC(1/4)
SfcI 730 CTGCAG C^T[GA][CT]AG
SfoI 1058 GGCGCC GGC^GCC
SmlI 101 CTCGAG C^T[CT][GA]AG
StyI 1144 CCAAGG C^C[AT][AT]GG
TfiI 1281 GAATC G^A[AT]TC
Tth111I 621 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 422 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1204 CCACTGCCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 101 CTCGAG C^TCGAG
XhoII 692 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 322 GAATTT [GA]^AATT[CT]
576 AAATTC
AocI 259 CCTGAGG CC^T[ACGT]AGG
482 CCTCAGG
ApoI 322 GAATTT [GA]^AATT[CT]
576 AAATTC
BanI 891 GGTGCC G^G[CT][GA]CC
1058 GGCGCC
BanII 98 GGGCTC G[GA]GC[CT]^C
588 GAGCCC
BfaI 374 CTAG C^TAG
1113 CTAG
BsaI 968 GAGACC GGTCTC(1/5)
1244 GGTCTC
BsrBI 702 GAGCGG CCGCTC(-3/-3)
846 GAGCGG
BsrFI 859 ACCGGC [GA]^CCGG[CT]
1055 GCCGGC
BsrI 1080 ACTGG ACTGG(1/-1)
976 CCAGT
Bsu36I 259 CCTGAGG CC^T[ACGT]AGG
482 CCTCAGG
Cfr10I 859 ACCGGC [GA]^CCGG[CT]
1055 GCCGGC
Eco81I 259 CCTGAGG CC^T[ACGT]AGG
482 CCTCAGG
MaeI 374 CTAG C^TAG
1113 CTAG
PleI 187 GAGTC GAGTC(4/5)
1170 GAGTC
SmaI 591 CCCGGG CCC^GGG
697 CCCGGG
TaqI 102 TCGA T^CGA
1269 TCGA
Tsp509I 323 AATT ^AATT
577 AATT
TthHB8I 102 TCGA T^CGA
1269 TCGA
XmaCI 591 CCCGGG C^CCGGG
697 CCCGGG
XmaI 591 CCCGGG C^CCGGG
697 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AvaI BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraIII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I TspRI