################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BseAI BseRI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HpaI KpnI MamI MfeI MluI MroI MslI MunI NcoI NdeI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccII 887 CGCG CG^CG AcyI 1058 GGCGCC G[GA]^CG[CT]C AfaI 767 GTAC GT^AC AlwI 693 GATCC GGATC(4/5) AlwNI 932 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 1011 GAGCAC G[AT]GC[AT]^C AspI 621 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 407 GGACC G^G[AT]CC BalI 758 TGGCCA TGG^CCA BbeI 1058 GGCGCC GGCGC^C BcgI 1013 GCACTTCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 527 GCTCAGC GC^T[ACGT]AGC BpmI 300 CTGGAG CTGGAG(16/14) Bpu1102I 527 GCTCAGC GC^T[ACGT]AGC BsaHI 1058 GGCGCC G[GA]^CG[CT]C BsaWI 964 ACCGGA [AT]^CCGG[AT] BsiHKAI 1011 GAGCAC G[AT]GC[AT]^C BsmBI 626 CGTCTC CGTCTC(1/5) BspMI 832 GCAGGT ACCTGC(4/8) BstAPI 1287 GCATGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 887 CGCG CG^CG BstXI 781 CCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 692 AGATCC [GA]^GATC[CT] CelII 527 GCTCAGC GC^T[ACGT]AGC DsaI 886 CCGCGG C^C[GA][CT]GG EaeI 758 TGGCCA [CT]^GGCC[GA] EarI 1008 GAAGAG CTCTTC(1/4) EcoNI 1177 CCTCACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1144 CCAAGG C^C[AT][AT]GG HaeII 1058 GGCGCC [GA]GCGC^[CT] Hin1I 1058 GGCGCC G[GA]^CG[CT]C HincII 672 GTCAAC GT[CT]^[GA]AC HindII 672 GTCAAC GT[CT]^[GA]AC HindIII 475 AAGCTT A^AGCTT KasI 1058 GGCGCC G^GCGCC Ksp632I 1008 GAAGAG CTCTTC(1/4) KspI 886 CCGCGG CCGC^GG MaeII 625 ACGT A^CGT MflI 692 AGATCC [GA]^GATC[CT] MluNI 758 TGGCCA TGG^CCA MscI 758 TGGCCA TGG^CCA MvnI 887 CGCG CG^CG NaeI 1055 GCCGGC GCC^GGC NarI 1058 GGCGCC GG^CGCC NgoMIV 1055 GCCGGC G^CCGGC NheI 373 GCTAGC G^CTAGC PaeR7I 101 CTCGAG C^TCGAG PflFI 621 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 422 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 730 CTGCAG CTGCA^G PvuII 838 CAGCTG CAG^CTG RsaI 767 GTAC GT^AC SacII 886 CCGCGG CCGC^GG SapI 1008 GAAGAGC GCTCTTC(1/4) SfcI 730 CTGCAG C^T[GA][CT]AG SfoI 1058 GGCGCC GGC^GCC SmlI 101 CTCGAG C^T[CT][GA]AG StyI 1144 CCAAGG C^C[AT][AT]GG TfiI 1281 GAATC G^A[AT]TC Tth111I 621 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 422 CCAATACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1204 CCACTGCCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 101 CTCGAG C^TCGAG XhoII 692 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 322 GAATTT [GA]^AATT[CT] 576 AAATTC AocI 259 CCTGAGG CC^T[ACGT]AGG 482 CCTCAGG ApoI 322 GAATTT [GA]^AATT[CT] 576 AAATTC BanI 891 GGTGCC G^G[CT][GA]CC 1058 GGCGCC BanII 98 GGGCTC G[GA]GC[CT]^C 588 GAGCCC BfaI 374 CTAG C^TAG 1113 CTAG BsaI 968 GAGACC GGTCTC(1/5) 1244 GGTCTC BsrBI 702 GAGCGG CCGCTC(-3/-3) 846 GAGCGG BsrFI 859 ACCGGC [GA]^CCGG[CT] 1055 GCCGGC BsrI 1080 ACTGG ACTGG(1/-1) 976 CCAGT Bsu36I 259 CCTGAGG CC^T[ACGT]AGG 482 CCTCAGG Cfr10I 859 ACCGGC [GA]^CCGG[CT] 1055 GCCGGC Eco81I 259 CCTGAGG CC^T[ACGT]AGG 482 CCTCAGG MaeI 374 CTAG C^TAG 1113 CTAG PleI 187 GAGTC GAGTC(4/5) 1170 GAGTC SmaI 591 CCCGGG CCC^GGG 697 CCCGGG TaqI 102 TCGA T^CGA 1269 TCGA Tsp509I 323 AATT ^AATT 577 AATT TthHB8I 102 TCGA T^CGA 1269 TCGA XmaCI 591 CCCGGG C^CCGGG 697 CCCGGG XmaI 591 CCCGGG C^CCGGG 697 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AvaI BbsI BbvI BcnI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraIII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I TspRI