##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BanI BbeI
BbrPI BcgI BciVI BlnI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1713 GACGTC GACGT^C
AccI 1676 GTCTAC GT^[AC][GT]AC
AfaI 1096 GTAC GT^AC
AflII 898 CTTAAG C^TTAAG
AflIII 525 ACATGT A^C[GA][CT]GT
ApaI 695 GGGCCC GGGCC^C
AspI 1459 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 698 CCCGAG C^[CT]CG[GA]G
BamHI 2110 GGATCC G^GATCC
BbvCI 819 GCTGAGG CCTCAGC(-5/-2)
BclI 1670 TGATCA T^GATCA
BcnI 462 CCGGG CC^[GC]GG
BfrI 898 CTTAAG C^TTAAG
BglI 937 GCCATTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1321 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1321 GCTGAGC GC^T[ACGT]AGC
BsaI 1766 GAGACC GGTCTC(1/5)
BsmAI 1766 GAGAC GTCTC(1/5)
BsmFI 113 GGGAC GGGAC(10/14)
BsoBI 698 CCCGAG C^[CT]CG[GA]G
BspLU11I 525 ACATGT A^CATGT
BsrFI 1940 GCCGGT [GA]^CCGG[CT]
BstEII 1333 GGTAACC G^GT[ACGT]ACC
BstPI 1333 GGTAACC G^GT[ACGT]ACC
CelII 1321 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1940 GCCGGT [GA]^CCGG[CT]
DrdI 2236 GACGTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 657 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1333 GGTAACC G^GT[ACGT]ACC
EcoRI 2356 GAATTC G^AATTC
FbaI 1670 TGATCA T^GATCA
HgaI 1937 GACGC GACGC(5/10)
HindIII 1790 AAGCTT A^AGCTT
MfeI 155 CAATTG C^AATTG
MunI 155 CAATTG C^AATTG
NciI 462 CCGGG CC^[GC]GG
NcoI 518 CCATGG C^CATGG
NspI 525 ACATGT [GA]CATG^[CT]
PflFI 1459 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1118 CCAATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2048 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 1096 GTAC GT^AC
SapI 1549 GAAGAGC GCTCTTC(1/4)
StuI 635 AGGCCT AGG^CCT
Tth111I 1459 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1118 CCAATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 489 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1986 GAATTT [GA]^AATT[CT]
2356 GAATTC
AcyI 1713 GACGTC G[GA]^CG[CT]C
1937 GACGCC
AlwNI 836 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1012 CAGAAGCTG
AocI 1290 CCTTAGG CC^T[ACGT]AGG
1960 CCTGAGG
ApoI 1986 GAATTT [GA]^AATT[CT]
2356 GAATTC
AspHI 701 GAGCTC G[AT]GC[AT]^C
1306 GAGCTC
BbsI 348 GAAGAC GAAGAC(2/6)
1101 GAAGAC
BglII 199 AGATCT A^GATCT
1300 AGATCT
BpuAI 348 GAAGAC GAAGAC(2/6)
1101 GAAGAC
BsaHI 1713 GACGTC G[GA]^CG[CT]C
1937 GACGCC
BsiHKAI 701 GAGCTC G[AT]GC[AT]^C
1306 GAGCTC
BspMI 1479 ACCTGC ACCTGC(4/8)
1614 GCAGGT
BstXI 650 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
875 CCAGAATGATGG
Bsu36I 1290 CCTTAGG CC^T[ACGT]AGG
1960 CCTGAGG
Cac8I 50 GCCAGC GC[ACGT]^[ACGT]GC
964 GCCCGC
DraIII 2038 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2376 CACTGTGTG
DsaI 518 CCATGG C^C[GA][CT]GG
1123 CCGTGG
Eco81I 1290 CCTTAGG CC^T[ACGT]AGG
1960 CCTGAGG
HapII 462 CCGG C^CGG
1941 CCGG
Hin1I 1713 GACGTC G[GA]^CG[CT]C
1937 GACGCC
HincII 829 GTTGAC GT[CT]^[GA]AC
1456 GTTGAC
HindII 829 GTTGAC GT[CT]^[GA]AC
1456 GTTGAC
HpaII 462 CCGG C^CGG
1941 CCGG
MaeII 1714 ACGT A^CGT
2237 ACGT
MspI 462 CCGG C^CGG
1941 CCGG
SacI 701 GAGCTC GAGCT^C
1306 GAGCTC
SexAI 1460 ACCTGGT A^CC[AT]GGT
2039 ACCAGGT
SmlI 90 CTTGAG C^T[CT][GA]AG
898 CTTAAG
TaqI 343 TCGA T^CGA
732 TCGA
TthHB8I 343 TCGA T^CGA
732 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII