################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1713 GACGTC GACGT^C AccI 1676 GTCTAC GT^[AC][GT]AC AfaI 1096 GTAC GT^AC AflII 898 CTTAAG C^TTAAG AflIII 525 ACATGT A^C[GA][CT]GT ApaI 695 GGGCCC GGGCC^C AspI 1459 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 698 CCCGAG C^[CT]CG[GA]G BamHI 2110 GGATCC G^GATCC BbvCI 819 GCTGAGG CCTCAGC(-5/-2) BclI 1670 TGATCA T^GATCA BcnI 462 CCGGG CC^[GC]GG BfrI 898 CTTAAG C^TTAAG BglI 937 GCCATTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1321 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1321 GCTGAGC GC^T[ACGT]AGC BsaI 1766 GAGACC GGTCTC(1/5) BsmAI 1766 GAGAC GTCTC(1/5) BsmFI 113 GGGAC GGGAC(10/14) BsoBI 698 CCCGAG C^[CT]CG[GA]G BspLU11I 525 ACATGT A^CATGT BsrFI 1940 GCCGGT [GA]^CCGG[CT] BstEII 1333 GGTAACC G^GT[ACGT]ACC BstPI 1333 GGTAACC G^GT[ACGT]ACC CelII 1321 GCTGAGC GC^T[ACGT]AGC Cfr10I 1940 GCCGGT [GA]^CCGG[CT] DrdI 2236 GACGTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 657 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1333 GGTAACC G^GT[ACGT]ACC EcoRI 2356 GAATTC G^AATTC FbaI 1670 TGATCA T^GATCA HgaI 1937 GACGC GACGC(5/10) HindIII 1790 AAGCTT A^AGCTT MfeI 155 CAATTG C^AATTG MunI 155 CAATTG C^AATTG NciI 462 CCGGG CC^[GC]GG NcoI 518 CCATGG C^CATGG NspI 525 ACATGT [GA]CATG^[CT] PflFI 1459 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1118 CCAATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2048 GGGTCCT [GA]G^G[AT]CC[CT] RsaI 1096 GTAC GT^AC SapI 1549 GAAGAGC GCTCTTC(1/4) StuI 635 AGGCCT AGG^CCT Tth111I 1459 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1118 CCAATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 489 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 1986 GAATTT [GA]^AATT[CT] 2356 GAATTC AcyI 1713 GACGTC G[GA]^CG[CT]C 1937 GACGCC AlwNI 836 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 1012 CAGAAGCTG AocI 1290 CCTTAGG CC^T[ACGT]AGG 1960 CCTGAGG ApoI 1986 GAATTT [GA]^AATT[CT] 2356 GAATTC AspHI 701 GAGCTC G[AT]GC[AT]^C 1306 GAGCTC BbsI 348 GAAGAC GAAGAC(2/6) 1101 GAAGAC BglII 199 AGATCT A^GATCT 1300 AGATCT BpuAI 348 GAAGAC GAAGAC(2/6) 1101 GAAGAC BsaHI 1713 GACGTC G[GA]^CG[CT]C 1937 GACGCC BsiHKAI 701 GAGCTC G[AT]GC[AT]^C 1306 GAGCTC BspMI 1479 ACCTGC ACCTGC(4/8) 1614 GCAGGT BstXI 650 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 875 CCAGAATGATGG Bsu36I 1290 CCTTAGG CC^T[ACGT]AGG 1960 CCTGAGG Cac8I 50 GCCAGC GC[ACGT]^[ACGT]GC 964 GCCCGC DraIII 2038 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2376 CACTGTGTG DsaI 518 CCATGG C^C[GA][CT]GG 1123 CCGTGG Eco81I 1290 CCTTAGG CC^T[ACGT]AGG 1960 CCTGAGG HapII 462 CCGG C^CGG 1941 CCGG Hin1I 1713 GACGTC G[GA]^CG[CT]C 1937 GACGCC HincII 829 GTTGAC GT[CT]^[GA]AC 1456 GTTGAC HindII 829 GTTGAC GT[CT]^[GA]AC 1456 GTTGAC HpaII 462 CCGG C^CGG 1941 CCGG MaeII 1714 ACGT A^CGT 2237 ACGT MspI 462 CCGG C^CGG 1941 CCGG SacI 701 GAGCTC GAGCT^C 1306 GAGCTC SexAI 1460 ACCTGGT A^CC[AT]GGT 2039 ACCAGGT SmlI 90 CTTGAG C^T[CT][GA]AG 898 CTTAAG TaqI 343 TCGA T^CGA 732 TCGA TthHB8I 343 TCGA T^CGA 732 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII