##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 236 GTCTAC GT^[AC][GT]AC
AcyI 713 GACGCC G[GA]^CG[CT]C
AflIII 255 ACGTGT A^C[GA][CT]GT
Alw44I 266 GTGCAC G^TGCAC
AocI 609 CCTCAGG CC^T[ACGT]AGG
ApaI 70 GGGCCC GGGCC^C
ApaLI 266 GTGCAC G^TGCAC
AscI 1047 GGCGCGCC GG^CGCGCC
AviII 426 TGCGCA TGC^GCA
BalI 483 TGGCCA TGG^CCA
BciVI 873 GTATCC GTATCC(6/5)
BglI 1052 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 417 AGATCT A^GATCT
BlpI 1067 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1067 GCTGAGC GC^T[ACGT]AGC
BsaHI 713 GACGCC G[GA]^CG[CT]C
Bsp1407I 258 TGTACA T^GTACA
BsrGI 258 TGTACA T^GTACA
Bsu36I 609 CCTCAGG CC^T[ACGT]AGG
CelII 1067 GCTGAGC GC^T[ACGT]AGC
DraIII 269 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 831 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 432 CCACGG C^C[GA][CT]GG
EagI 1332 CGGCCG C^GGCCG
EarI 403 GAAGAG CTCTTC(1/4)
EclXI 1332 CGGCCG C^GGCCG
Eco52I 1332 CGGCCG C^GGCCG
Eco81I 609 CCTCAGG CC^T[ACGT]AGG
FspI 426 TGCGCA TGC^GCA
Hin1I 713 GACGCC G[GA]^CG[CT]C
Ksp632I 403 GAAGAG CTCTTC(1/4)
MluNI 483 TGGCCA TGG^CCA
MscI 483 TGGCCA TGG^CCA
MseI 558 TTAA T^TAA
NruI 1401 TCGCGA TCG^CGA
NspI 1231 ACATGC [GA]CATG^[CT]
PstI 1260 CTGCAG CTGCA^G
SfcI 1260 CTGCAG C^T[GA][CT]AG
SgrAI 521 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1053 CCCGGG CCC^GGG
SspBI 258 TGTACA T^GTACA
StuI 613 AGGCCT AGG^CCT
TfiI 1112 GATTC G^A[AT]TC
Tru9I 558 TTAA T^TAA
Tsp509I 1409 AATT ^AATT
XmaCI 1053 CCCGGG C^CCGGG
XmaI 1053 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 259 GTAC GT^AC
374 GTAC
AlwI 564 GGATC GGATC(4/5)
653 GGATC
AlwNI 18 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
777 CAGCCACTG
AspHI 266 GTGCAC G[AT]GC[AT]^C
584 GTGCTC
AvaI 123 CCCGAG C^[CT]CG[GA]G
1053 CCCGGG
AvrII 971 CCTAGG C^CTAGG
1009 CCTAGG
BlnI 971 CCTAGG C^CTAGG
1009 CCTAGG
BsaI 530 GAGACC GGTCTC(1/5)
966 GGTCTC
BsiEI 850 CGGTCG CG[GA][CT]^CG
1332 CGGCCG
BsiHKAI 266 GTGCAC G[AT]GC[AT]^C
584 GTGCTC
BsoBI 123 CCCGAG C^[CT]CG[GA]G
1053 CCCGGG
BspHI 78 TCATGA T^CATGA
1080 TCATGA
BsrBI 97 CCGCTC CCGCTC(-3/-3)
847 GAGCGG
BssHII 223 GCGCGC G^CGCGC
1048 GCGCGC
BstXI 432 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
453 CCATCAAGGTGG
BstYI 417 AGATCT [GA]^GATC[CT]
564 GGATCT
EaeI 483 TGGCCA [CT]^GGCC[GA]
1332 CGGCCG
HgaI 713 GACGC GACGC(5/10)
1105 GCGTC
MflI 417 AGATCT [GA]^GATC[CT]
564 GGATCT
MspA1I 64 CAGCGG C[AC]G^C[GT]G
887 CCGCTG
NaeI 522 GCCGGC GCC^GGC
1044 GCCGGC
NgoMIV 522 GCCGGC G^CCGGC
1044 GCCGGC
PflMI 155 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCAGCCACTGG
RcaI 78 TCATGA T^CATGA
1080 TCATGA
RsaI 259 GTAC GT^AC
374 GTAC
SfaNI 731 GCATC GCATC(5/9)
1093 GCATC
TaqI 4 TCGA T^CGA
1152 TCGA
TthHB8I 4 TCGA T^CGA
1152 TCGA
Van91I 155 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCAGCCACTGG
XcmI 486 CCAAGGGCCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1017 CCAGTGAGAGGCTGG
XhoII 417 AGATCT [GA]^GATC[CT]
564 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI StyI TseI Tsp45I TspRI