################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AseI AsnI Asp700I Asp718I AspEI AspI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 236 GTCTAC GT^[AC][GT]AC AcyI 713 GACGCC G[GA]^CG[CT]C AflIII 255 ACGTGT A^C[GA][CT]GT Alw44I 266 GTGCAC G^TGCAC AocI 609 CCTCAGG CC^T[ACGT]AGG ApaI 70 GGGCCC GGGCC^C ApaLI 266 GTGCAC G^TGCAC AscI 1047 GGCGCGCC GG^CGCGCC AviII 426 TGCGCA TGC^GCA BalI 483 TGGCCA TGG^CCA BciVI 873 GTATCC GTATCC(6/5) BglI 1052 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 417 AGATCT A^GATCT BlpI 1067 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1067 GCTGAGC GC^T[ACGT]AGC BsaHI 713 GACGCC G[GA]^CG[CT]C Bsp1407I 258 TGTACA T^GTACA BsrGI 258 TGTACA T^GTACA Bsu36I 609 CCTCAGG CC^T[ACGT]AGG CelII 1067 GCTGAGC GC^T[ACGT]AGC DraIII 269 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 831 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 432 CCACGG C^C[GA][CT]GG EagI 1332 CGGCCG C^GGCCG EarI 403 GAAGAG CTCTTC(1/4) EclXI 1332 CGGCCG C^GGCCG Eco52I 1332 CGGCCG C^GGCCG Eco81I 609 CCTCAGG CC^T[ACGT]AGG FspI 426 TGCGCA TGC^GCA Hin1I 713 GACGCC G[GA]^CG[CT]C Ksp632I 403 GAAGAG CTCTTC(1/4) MluNI 483 TGGCCA TGG^CCA MscI 483 TGGCCA TGG^CCA MseI 558 TTAA T^TAA NruI 1401 TCGCGA TCG^CGA NspI 1231 ACATGC [GA]CATG^[CT] PstI 1260 CTGCAG CTGCA^G SfcI 1260 CTGCAG C^T[GA][CT]AG SgrAI 521 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1053 CCCGGG CCC^GGG SspBI 258 TGTACA T^GTACA StuI 613 AGGCCT AGG^CCT TfiI 1112 GATTC G^A[AT]TC Tru9I 558 TTAA T^TAA Tsp509I 1409 AATT ^AATT XmaCI 1053 CCCGGG C^CCGGG XmaI 1053 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 259 GTAC GT^AC 374 GTAC AlwI 564 GGATC GGATC(4/5) 653 GGATC AlwNI 18 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 777 CAGCCACTG AspHI 266 GTGCAC G[AT]GC[AT]^C 584 GTGCTC AvaI 123 CCCGAG C^[CT]CG[GA]G 1053 CCCGGG AvrII 971 CCTAGG C^CTAGG 1009 CCTAGG BlnI 971 CCTAGG C^CTAGG 1009 CCTAGG BsaI 530 GAGACC GGTCTC(1/5) 966 GGTCTC BsiEI 850 CGGTCG CG[GA][CT]^CG 1332 CGGCCG BsiHKAI 266 GTGCAC G[AT]GC[AT]^C 584 GTGCTC BsoBI 123 CCCGAG C^[CT]CG[GA]G 1053 CCCGGG BspHI 78 TCATGA T^CATGA 1080 TCATGA BsrBI 97 CCGCTC CCGCTC(-3/-3) 847 GAGCGG BssHII 223 GCGCGC G^CGCGC 1048 GCGCGC BstXI 432 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 453 CCATCAAGGTGG BstYI 417 AGATCT [GA]^GATC[CT] 564 GGATCT EaeI 483 TGGCCA [CT]^GGCC[GA] 1332 CGGCCG HgaI 713 GACGC GACGC(5/10) 1105 GCGTC MflI 417 AGATCT [GA]^GATC[CT] 564 GGATCT MspA1I 64 CAGCGG C[AC]G^C[GT]G 887 CCGCTG NaeI 522 GCCGGC GCC^GGC 1044 GCCGGC NgoMIV 522 GCCGGC G^CCGGC 1044 GCCGGC PflMI 155 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 776 CCAGCCACTGG RcaI 78 TCATGA T^CATGA 1080 TCATGA RsaI 259 GTAC GT^AC 374 GTAC SfaNI 731 GCATC GCATC(5/9) 1093 GCATC TaqI 4 TCGA T^CGA 1152 TCGA TthHB8I 4 TCGA T^CGA 1152 TCGA Van91I 155 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 776 CCAGCCACTGG XcmI 486 CCAAGGGCCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1017 CCAGTGAGAGGCTGG XhoII 417 AGATCT [GA]^GATC[CT] 564 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AluI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI StyI TseI Tsp45I TspRI