##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BamHI BanII
BbeI BbsI BbvCI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaBI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 139 GACGTC GACGT^C
AccII 456 CGCG CG^CG
AccIII 1241 TCCGGA T^CCGGA
AcyI 139 GACGTC G[GA]^CG[CT]C
AflIII 830 ACGTGT A^C[GA][CT]GT
Alw44I 40 GTGCAC G^TGCAC
AocI 1429 CCTCAGG CC^T[ACGT]AGG
ApaLI 40 GTGCAC G^TGCAC
AvaI 179 CTCGGG C^[CT]CG[GA]G
AvrII 1257 CCTAGG C^CTAGG
BalI 164 TGGCCA TGG^CCA
BanI 328 GGCACC G^G[CT][GA]CC
BcgI 741 CGAGAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 977 GGATAC GTATCC(6/5)
BclI 825 TGATCA T^GATCA
BlnI 1257 CCTAGG C^CTAGG
BsaHI 139 GACGTC G[GA]^CG[CT]C
BsaI 1366 GAGACC GGTCTC(1/5)
BseAI 1241 TCCGGA T^CCGGA
BseRI 717 GAGGAG GAGGAG(10/8)
BsgI 866 CTGCAC GTGCAG(16/14)
BsmBI 205 GAGACG CGTCTC(1/5)
BsoBI 179 CTCGGG C^[CT]CG[GA]G
BspEI 1241 TCCGGA T^CCGGA
BsrBI 720 GAGCGG CCGCTC(-3/-3)
BsrFI 969 ACCGGC [GA]^CCGG[CT]
BstAPI 16 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 456 CGCG CG^CG
Bsu36I 1429 CCTCAGG CC^T[ACGT]AGG
Cfr10I 969 ACCGGC [GA]^CCGG[CT]
EaeI 164 TGGCCA [CT]^GGCC[GA]
Eco81I 1429 CCTCAGG CC^T[ACGT]AGG
EcoRV 184 GATATC GAT^ATC
FbaI 825 TGATCA T^GATCA
HgaI 75 GACGC GACGC(5/10)
Hin1I 139 GACGTC G[GA]^CG[CT]C
HincII 559 GTCAAC GT[CT]^[GA]AC
HindII 559 GTCAAC GT[CT]^[GA]AC
HindIII 121 AAGCTT A^AGCTT
MluNI 164 TGGCCA TGG^CCA
MroI 1241 TCCGGA T^CCGGA
MscI 164 TGGCCA TGG^CCA
MslI 276 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 456 CGCG CG^CG
PvuII 64 CAGCTG CAG^CTG
ScaI 638 AGTACT AGT^ACT
SpeI 786 ACTAGT A^CTAGT
SspI 874 AATATT AAT^ATT
XcmI 1335 CCATGGCCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 1248 GATCC GGATC(4/5)
404 GGATC
AlwNI 17 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
547 CAGCCCCTG
AspHI 40 GTGCAC G[AT]GC[AT]^C
960 GAGCAC
BbrPI 43 CACGTG CAC^GTG
829 CACGTG
BcnI 732 CCGGG CC^[GC]GG
925 CCGGG
BmyI 40 GTGCAC G[AGT]GC[ACT]^C
960 GAGCAC
BpmI 253 CTGGAG CTGGAG(16/14)
1359 CTGGAG
BsaAI 43 CACGTG [CT]AC^GT[GA]
829 CACGTG
BsaWI 188 TCCGGT [AT]^CCGG[AT]
1241 TCCGGA
BsiHKAI 40 GTGCAC G[AT]GC[AT]^C
960 GAGCAC
BsmI 1123 GAATGC GAATGC(1/-1)
1466 GCATTC
Bsp1286I 40 GTGCAC G[AGT]GC[ACT]^C
960 GAGCAC
BstYI 404 GGATCT [GA]^GATC[CT]
1247 AGATCC
FauI 1209 CCCGC CCCGC(4/6)
1314 CCCGC
MflI 404 GGATCT [GA]^GATC[CT]
1247 AGATCC
MspA1I 64 CAGCTG C[AC]G^C[GT]G
852 CCGCTG
NciI 732 CCGGG CC^[GC]GG
925 CCGGG
NspI 69 GCATGT [GA]CATG^[CT]
695 ACATGC
PleI 557 GAGTC GAGTC(4/5)
1425 GAGTC
PmaCI 43 CACGTG CAC^GTG
829 CACGTG
PmlI 43 CACGTG CAC^GTG
829 CACGTG
PstI 14 CTGCAG CTGCA^G
998 CTGCAG
TaqI 915 TCGA T^CGA
1175 TCGA
Tsp45I 73 GTGAC ^GT[GC]AC
1102 GTGAC
TthHB8I 915 TCGA T^CGA
1175 TCGA
XhoII 404 GGATCT [GA]^GATC[CT]
1247 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp509I TspRI