##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        AhdI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       BamHI       BanII       
BbeI        BbsI        BbvCI       BfrI        BglI        
BglII       BlpI        Bpu1102I    BpuAI       BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       CfoI        ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoT22I     FseI        FspI        HaeII       HhaI        
HinP1I      HpaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspBI       StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         139  GACGTC               GACGT^C
AccII         456  CGCG                 CG^CG
AccIII       1241  TCCGGA               T^CCGGA
AcyI          139  GACGTC               G[GA]^CG[CT]C
AflIII        830  ACGTGT               A^C[GA][CT]GT
Alw44I         40  GTGCAC               G^TGCAC
AocI         1429  CCTCAGG              CC^T[ACGT]AGG
ApaLI          40  GTGCAC               G^TGCAC
AvaI          179  CTCGGG               C^[CT]CG[GA]G
AvrII        1257  CCTAGG               C^CTAGG
BalI          164  TGGCCA               TGG^CCA
BanI          328  GGCACC               G^G[CT][GA]CC
BcgI          741  CGAGAAAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         977  GGATAC               GTATCC(6/5)
BclI          825  TGATCA               T^GATCA
BlnI         1257  CCTAGG               C^CTAGG
BsaHI         139  GACGTC               G[GA]^CG[CT]C
BsaI         1366  GAGACC               GGTCTC(1/5)
BseAI        1241  TCCGGA               T^CCGGA
BseRI         717  GAGGAG               GAGGAG(10/8)
BsgI          866  CTGCAC               GTGCAG(16/14)
BsmBI         205  GAGACG               CGTCTC(1/5)
BsoBI         179  CTCGGG               C^[CT]CG[GA]G
BspEI        1241  TCCGGA               T^CCGGA
BsrBI         720  GAGCGG               CCGCTC(-3/-3)
BsrFI         969  ACCGGC               [GA]^CCGG[CT]
BstAPI         16  GCAGAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         456  CGCG                 CG^CG
Bsu36I       1429  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        969  ACCGGC               [GA]^CCGG[CT]
EaeI          164  TGGCCA               [CT]^GGCC[GA]
Eco81I       1429  CCTCAGG              CC^T[ACGT]AGG
EcoRV         184  GATATC               GAT^ATC
FbaI          825  TGATCA               T^GATCA
HgaI           75  GACGC                GACGC(5/10)
Hin1I         139  GACGTC               G[GA]^CG[CT]C
HincII        559  GTCAAC               GT[CT]^[GA]AC
HindII        559  GTCAAC               GT[CT]^[GA]AC
HindIII       121  AAGCTT               A^AGCTT
MluNI         164  TGGCCA               TGG^CCA
MroI         1241  TCCGGA               T^CCGGA
MscI          164  TGGCCA               TGG^CCA
MslI          276  CATCTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          456  CGCG                 CG^CG
PvuII          64  CAGCTG               CAG^CTG
ScaI          638  AGTACT               AGT^ACT
SpeI          786  ACTAGT               A^CTAGT
SspI          874  AATATT               AAT^ATT
XcmI         1335  CCATGGCCCCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI         1248  GATCC                GGATC(4/5)
              404  GGATC               
AlwNI          17  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              547  CAGCCCCTG           
AspHI          40  GTGCAC               G[AT]GC[AT]^C
              960  GAGCAC              
BbrPI          43  CACGTG               CAC^GTG
              829  CACGTG              
BcnI          732  CCGGG                CC^[GC]GG
              925  CCGGG               
BmyI           40  GTGCAC               G[AGT]GC[ACT]^C
              960  GAGCAC              
BpmI          253  CTGGAG               CTGGAG(16/14)
             1359  CTGGAG              
BsaAI          43  CACGTG               [CT]AC^GT[GA]
              829  CACGTG              
BsaWI         188  TCCGGT               [AT]^CCGG[AT]
             1241  TCCGGA              
BsiHKAI        40  GTGCAC               G[AT]GC[AT]^C
              960  GAGCAC              
BsmI         1123  GAATGC               GAATGC(1/-1)
             1466  GCATTC              
Bsp1286I       40  GTGCAC               G[AGT]GC[ACT]^C
              960  GAGCAC              
BstYI         404  GGATCT               [GA]^GATC[CT]
             1247  AGATCC              
FauI         1209  CCCGC                CCCGC(4/6)
             1314  CCCGC               
MflI          404  GGATCT               [GA]^GATC[CT]
             1247  AGATCC              
MspA1I         64  CAGCTG               C[AC]G^C[GT]G
              852  CCGCTG              
NciI          732  CCGGG                CC^[GC]GG
              925  CCGGG               
NspI           69  GCATGT               [GA]CATG^[CT]
              695  ACATGC              
PleI          557  GAGTC                GAGTC(4/5)
             1425  GAGTC               
PmaCI          43  CACGTG               CAC^GTG
              829  CACGTG              
PmlI           43  CACGTG               CAC^GTG
              829  CACGTG              
PstI           14  CTGCAG               CTGCA^G
              998  CTGCAG              
TaqI          915  TCGA                 T^CGA
             1175  TCGA                
Tsp45I         73  GTGAC                ^GT[GC]AC
             1102  GTGAC               
TthHB8I       915  TCGA                 T^CGA
             1175  TCGA                
XhoII         404  GGATCT               [GA]^GATC[CT]
             1247  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BfaI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        TseI        Tsp509I     TspRI