################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BamHI BanII BbeI BbsI BbvCI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaBI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 139 GACGTC GACGT^C AccII 456 CGCG CG^CG AccIII 1241 TCCGGA T^CCGGA AcyI 139 GACGTC G[GA]^CG[CT]C AflIII 830 ACGTGT A^C[GA][CT]GT Alw44I 40 GTGCAC G^TGCAC AocI 1429 CCTCAGG CC^T[ACGT]AGG ApaLI 40 GTGCAC G^TGCAC AvaI 179 CTCGGG C^[CT]CG[GA]G AvrII 1257 CCTAGG C^CTAGG BalI 164 TGGCCA TGG^CCA BanI 328 GGCACC G^G[CT][GA]CC BcgI 741 CGAGAAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 977 GGATAC GTATCC(6/5) BclI 825 TGATCA T^GATCA BlnI 1257 CCTAGG C^CTAGG BsaHI 139 GACGTC G[GA]^CG[CT]C BsaI 1366 GAGACC GGTCTC(1/5) BseAI 1241 TCCGGA T^CCGGA BseRI 717 GAGGAG GAGGAG(10/8) BsgI 866 CTGCAC GTGCAG(16/14) BsmBI 205 GAGACG CGTCTC(1/5) BsoBI 179 CTCGGG C^[CT]CG[GA]G BspEI 1241 TCCGGA T^CCGGA BsrBI 720 GAGCGG CCGCTC(-3/-3) BsrFI 969 ACCGGC [GA]^CCGG[CT] BstAPI 16 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 456 CGCG CG^CG Bsu36I 1429 CCTCAGG CC^T[ACGT]AGG Cfr10I 969 ACCGGC [GA]^CCGG[CT] EaeI 164 TGGCCA [CT]^GGCC[GA] Eco81I 1429 CCTCAGG CC^T[ACGT]AGG EcoRV 184 GATATC GAT^ATC FbaI 825 TGATCA T^GATCA HgaI 75 GACGC GACGC(5/10) Hin1I 139 GACGTC G[GA]^CG[CT]C HincII 559 GTCAAC GT[CT]^[GA]AC HindII 559 GTCAAC GT[CT]^[GA]AC HindIII 121 AAGCTT A^AGCTT MluNI 164 TGGCCA TGG^CCA MroI 1241 TCCGGA T^CCGGA MscI 164 TGGCCA TGG^CCA MslI 276 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 456 CGCG CG^CG PvuII 64 CAGCTG CAG^CTG ScaI 638 AGTACT AGT^ACT SpeI 786 ACTAGT A^CTAGT SspI 874 AATATT AAT^ATT XcmI 1335 CCATGGCCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 1248 GATCC GGATC(4/5) 404 GGATC AlwNI 17 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 547 CAGCCCCTG AspHI 40 GTGCAC G[AT]GC[AT]^C 960 GAGCAC BbrPI 43 CACGTG CAC^GTG 829 CACGTG BcnI 732 CCGGG CC^[GC]GG 925 CCGGG BmyI 40 GTGCAC G[AGT]GC[ACT]^C 960 GAGCAC BpmI 253 CTGGAG CTGGAG(16/14) 1359 CTGGAG BsaAI 43 CACGTG [CT]AC^GT[GA] 829 CACGTG BsaWI 188 TCCGGT [AT]^CCGG[AT] 1241 TCCGGA BsiHKAI 40 GTGCAC G[AT]GC[AT]^C 960 GAGCAC BsmI 1123 GAATGC GAATGC(1/-1) 1466 GCATTC Bsp1286I 40 GTGCAC G[AGT]GC[ACT]^C 960 GAGCAC BstYI 404 GGATCT [GA]^GATC[CT] 1247 AGATCC FauI 1209 CCCGC CCCGC(4/6) 1314 CCCGC MflI 404 GGATCT [GA]^GATC[CT] 1247 AGATCC MspA1I 64 CAGCTG C[AC]G^C[GT]G 852 CCGCTG NciI 732 CCGGG CC^[GC]GG 925 CCGGG NspI 69 GCATGT [GA]CATG^[CT] 695 ACATGC PleI 557 GAGTC GAGTC(4/5) 1425 GAGTC PmaCI 43 CACGTG CAC^GTG 829 CACGTG PmlI 43 CACGTG CAC^GTG 829 CACGTG PstI 14 CTGCAG CTGCA^G 998 CTGCAG TaqI 915 TCGA T^CGA 1175 TCGA Tsp45I 73 GTGAC ^GT[GC]AC 1102 GTGAC TthHB8I 915 TCGA T^CGA 1175 TCGA XhoII 404 GGATCT [GA]^GATC[CT] 1247 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BfaI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp509I TspRI