##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AviII BamHI BbeI
BbrPI BcgI BciVI BfrI BglI
BglII BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI FspI HgaI
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SspI SwaI
TfiI XbaI XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 527 GTAGAC GT^[AC][GT]AC
AcsI 1762 GAATTT [GA]^AATT[CT]
AflIII 1466 ACGTGT A^C[GA][CT]GT
AocI 1602 CCTGAGG CC^T[ACGT]AGG
ApoI 1762 GAATTT [GA]^AATT[CT]
AseI 1754 ATTAAT AT^TAAT
AsnI 1754 ATTAAT AT^TAAT
AspI 782 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1670 CTCGAG C^[CT]CG[GA]G
AvrII 442 CCTAGG C^CTAGG
BclI 1023 TGATCA T^GATCA
BcnI 538 CCGGG CC^[GC]GG
BlnI 442 CCTAGG C^CTAGG
BsaBI 191 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1670 CTCGAG C^[CT]CG[GA]G
BspHI 1026 TCATGA T^CATGA
BssSI 884 CACGAG CACGAG(-5/-1)
BstAPI 805 GCACAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1075 GGTCACC G^GT[ACGT]ACC
BstPI 1075 GGTCACC G^GT[ACGT]ACC
BstYI 201 GGATCT [GA]^GATC[CT]
Bsu36I 1602 CCTGAGG CC^T[ACGT]AGG
DrdI 1000 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 456 CCATGG C^C[GA][CT]GG
Eco81I 1602 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1075 GGTCACC G^GT[ACGT]ACC
EcoT22I 1270 ATGCAT ATGCA^T
FauI 1195 CCCGC CCCGC(4/6)
FbaI 1023 TGATCA T^GATCA
HaeII 1423 AGCGCC [GA]GCGC^[CT]
HincII 1793 GTTAAC GT[CT]^[GA]AC
HindII 1793 GTTAAC GT[CT]^[GA]AC
HindIII 797 AAGCTT A^AGCTT
HpaI 1793 GTTAAC GTT^AAC
MamI 191 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 201 GGATCT [GA]^GATC[CT]
NciI 538 CCGGG CC^[GC]GG
NcoI 456 CCATGG C^CATGG
NsiI 1270 ATGCAT ATGCA^T
PaeR7I 1670 CTCGAG C^TCGAG
PflFI 782 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 784 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1754 ATTAAT AT^TAAT
RcaI 1026 TCATGA T^CATGA
SacI 1141 GAGCTC GAGCT^C
SapI 1198 GCTCTTC GCTCTTC(1/4)
SexAI 783 ACCAGGT A^CC[AT]GGT
SpeI 1283 ACTAGT A^CTAGT
StuI 1599 AGGCCT AGG^CCT
TaqI 1671 TCGA T^CGA
Tth111I 782 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1671 TCGA T^CGA
Van91I 784 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1670 CTCGAG C^TCGAG
XhoII 201 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 196 GGATC GGATC(4/5)
201 GGATC
BalI 45 TGGCCA TGG^CCA
1182 TGGCCA
BanI 541 GGCACC G^G[CT][GA]CC
879 GGTGCC
BbsI 997 GAAGAC GAAGAC(2/6)
1495 GAAGAC
BbvCI 1210 CCTCAGC CCTCAGC(-5/-2)
287 GCTGAGG
BpuAI 997 GAAGAC GAAGAC(2/6)
1495 GAAGAC
BsaI 1172 GAGACC GGTCTC(1/5)
1568 GAGACC
BsgI 157 CTGCAC GTGCAG(16/14)
450 CTGCAC
BsrBI 1196 CCGCTC CCGCTC(-3/-3)
467 GAGCGG
CfoI 964 GCGC GCG^C
1424 GCGC
DraIII 581 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1164 CACAGAGTG
EcoNI 652 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1576 CCTAGATCAGG
HhaI 964 GCGC GCG^C
1424 GCGC
HinP1I 964 GCGC G^CGC
1424 GCGC
MluNI 45 TGGCCA TGG^CCA
1182 TGGCCA
MscI 45 TGGCCA TGG^CCA
1182 TGGCCA
PvuII 498 CAGCTG CAG^CTG
608 CAGCTG
SmlI 800 CTTGAG C^T[CT][GA]AG
1670 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanII BbvI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI Cac8I CelII Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI StyI Tru9I
TseI Tsp45I Tsp509I TspRI