################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AviII BamHI BbeI BbrPI BcgI BciVI BfrI BglI BglII BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FseI FspI HgaI Hin1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI SwaI TfiI XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 527 GTAGAC GT^[AC][GT]AC AcsI 1762 GAATTT [GA]^AATT[CT] AflIII 1466 ACGTGT A^C[GA][CT]GT AocI 1602 CCTGAGG CC^T[ACGT]AGG ApoI 1762 GAATTT [GA]^AATT[CT] AseI 1754 ATTAAT AT^TAAT AsnI 1754 ATTAAT AT^TAAT AspI 782 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1670 CTCGAG C^[CT]CG[GA]G AvrII 442 CCTAGG C^CTAGG BclI 1023 TGATCA T^GATCA BcnI 538 CCGGG CC^[GC]GG BlnI 442 CCTAGG C^CTAGG BsaBI 191 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 1670 CTCGAG C^[CT]CG[GA]G BspHI 1026 TCATGA T^CATGA BssSI 884 CACGAG CACGAG(-5/-1) BstAPI 805 GCACAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1075 GGTCACC G^GT[ACGT]ACC BstPI 1075 GGTCACC G^GT[ACGT]ACC BstYI 201 GGATCT [GA]^GATC[CT] Bsu36I 1602 CCTGAGG CC^T[ACGT]AGG DrdI 1000 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 456 CCATGG C^C[GA][CT]GG Eco81I 1602 CCTGAGG CC^T[ACGT]AGG EcoO65I 1075 GGTCACC G^GT[ACGT]ACC EcoT22I 1270 ATGCAT ATGCA^T FauI 1195 CCCGC CCCGC(4/6) FbaI 1023 TGATCA T^GATCA HaeII 1423 AGCGCC [GA]GCGC^[CT] HincII 1793 GTTAAC GT[CT]^[GA]AC HindII 1793 GTTAAC GT[CT]^[GA]AC HindIII 797 AAGCTT A^AGCTT HpaI 1793 GTTAAC GTT^AAC MamI 191 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 201 GGATCT [GA]^GATC[CT] NciI 538 CCGGG CC^[GC]GG NcoI 456 CCATGG C^CATGG NsiI 1270 ATGCAT ATGCA^T PaeR7I 1670 CTCGAG C^TCGAG PflFI 782 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 784 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1754 ATTAAT AT^TAAT RcaI 1026 TCATGA T^CATGA SacI 1141 GAGCTC GAGCT^C SapI 1198 GCTCTTC GCTCTTC(1/4) SexAI 783 ACCAGGT A^CC[AT]GGT SpeI 1283 ACTAGT A^CTAGT StuI 1599 AGGCCT AGG^CCT TaqI 1671 TCGA T^CGA Tth111I 782 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1671 TCGA T^CGA Van91I 784 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1670 CTCGAG C^TCGAG XhoII 201 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 196 GGATC GGATC(4/5) 201 GGATC BalI 45 TGGCCA TGG^CCA 1182 TGGCCA BanI 541 GGCACC G^G[CT][GA]CC 879 GGTGCC BbsI 997 GAAGAC GAAGAC(2/6) 1495 GAAGAC BbvCI 1210 CCTCAGC CCTCAGC(-5/-2) 287 GCTGAGG BpuAI 997 GAAGAC GAAGAC(2/6) 1495 GAAGAC BsaI 1172 GAGACC GGTCTC(1/5) 1568 GAGACC BsgI 157 CTGCAC GTGCAG(16/14) 450 CTGCAC BsrBI 1196 CCGCTC CCGCTC(-3/-3) 467 GAGCGG CfoI 964 GCGC GCG^C 1424 GCGC DraIII 581 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1164 CACAGAGTG EcoNI 652 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1576 CCTAGATCAGG HhaI 964 GCGC GCG^C 1424 GCGC HinP1I 964 GCGC G^CGC 1424 GCGC MluNI 45 TGGCCA TGG^CCA 1182 TGGCCA MscI 45 TGGCCA TGG^CCA 1182 TGGCCA PvuII 498 CAGCTG CAG^CTG 608 CAGCTG SmlI 800 CTTGAG C^T[CT][GA]AG 1670 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I CelII Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI Tru9I TseI Tsp45I Tsp509I TspRI