##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI ApaI AscI
AseI AsnI Asp718I AspEI AviII
BanII BbeI BbrPI BcgI BciVI
BfrI BglII BsaAI BsaBI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstZ17I Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1056 GACGTC GACGT^C
AcyI 1056 GACGTC G[GA]^CG[CT]C
AfaI 1796 GTAC GT^AC
AocI 138 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1547 AGCGCT AGC^GCT
AspI 1893 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1730 CCTAGG C^CTAGG
BamHI 1641 GGATCC G^GATCC
BbvCI 1768 CCTCAGC CCTCAGC(-5/-2)
BclI 740 TGATCA T^GATCA
BglI 814 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1730 CCTAGG C^CTAGG
BlpI 511 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 511 GCTCAGC GC^T[ACGT]AGC
BsaHI 1056 GACGTC G[GA]^CG[CT]C
BsaWI 1040 ACCGGA [AT]^CCGG[AT]
BsmI 1063 GCATTC GAATGC(1/-1)
Bsp1407I 1795 TGTACA T^GTACA
BspHI 488 TCATGA T^CATGA
BsrGI 1795 TGTACA T^GTACA
BstBI 1535 TTCGAA TT^CGAA
BstEII 1722 GGTTACC G^GT[ACGT]ACC
BstPI 1722 GGTTACC G^GT[ACGT]ACC
Bsu36I 138 CCTCAGG CC^T[ACGT]AGG
CelII 511 GCTCAGC GC^T[ACGT]AGC
DraI 1099 TTTAAA TTT^AAA
DraII 1933 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 857 CCACGG C^C[GA][CT]GG
Eco47III 1547 AGCGCT AGC^GCT
Eco81I 138 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1933 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1722 GGTTACC G^GT[ACGT]ACC
FauI 352 GCGGG CCCGC(4/6)
FbaI 740 TGATCA T^GATCA
HaeII 1547 AGCGCT [GA]GCGC^[CT]
HgaI 183 GCGTC GACGC(5/10)
Hin1I 1056 GACGTC G[GA]^CG[CT]C
NspV 1535 TTCGAA TT^CGAA
PaeR7I 1492 CTCGAG C^TCGAG
PflFI 1893 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 180 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1933 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 488 TCATGA T^CATGA
RsaI 1796 GTAC GT^AC
SfuI 1535 TTCGAA TT^CGAA
SspBI 1795 TGTACA T^GTACA
StuI 1958 AGGCCT AGG^CCT
Tth111I 1893 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 180 CCAGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1492 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 833 CGCG CG^CG
1515 CGCG
AcsI 28 AAATTT [GA]^AATT[CT]
1510 AAATTC
Alw44I 388 GTGCAC G^TGCAC
1256 GTGCAC
ApaLI 388 GTGCAC G^TGCAC
1256 GTGCAC
ApoI 28 AAATTT [GA]^AATT[CT]
1510 AAATTC
BanI 231 GGTGCC G^G[CT][GA]CC
1072 GGCACC
BseRI 710 CTCCTC GAGGAG(10/8)
121 GAGGAG
BsrBI 167 GAGCGG CCGCTC(-3/-3)
358 GAGCGG
BstUI 833 CGCG CG^CG
1515 CGCG
BstXI 236 CCATCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
991 CCAGGAGACTGG
BstYI 716 AGATCC [GA]^GATC[CT]
1641 GGATCC
DrdI 82 GACTCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
748 GACCACGAAGTC
HincII 616 GTCAAC GT[CT]^[GA]AC
1676 GTCAAC
HindII 616 GTCAAC GT[CT]^[GA]AC
1676 GTCAAC
MflI 716 AGATCC [GA]^GATC[CT]
1641 GGATCC
MslI 237 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
381 CACAGATGTG
MvnI 833 CGCG CG^CG
1515 CGCG
PstI 579 CTGCAG CTGCA^G
1363 CTGCAG
SexAI 227 ACCTGGT A^CC[AT]GGT
1894 ACCAGGT
SmaI 307 CCCGGG CCC^GGG
1553 CCCGGG
SspI 2035 AATATT AAT^ATT
2112 AATATT
XhoII 716 AGATCC [GA]^GATC[CT]
1641 GGATCC
XmaCI 307 CCCGGG C^CCGGG
1553 CCCGGG
XmaI 307 CCCGGG C^CCGGG
1553 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AluI AlwI AlwNI Asp700I
AspHI AvaI AvaII BalI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraIII EaeI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmnI