##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI BstXI BstYI Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeII MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccI 846 GTATAC GT^[AC][GT]AC
AciI 69 GCGG CCGC(-3/-1)
AcyI 151 GGCGCC G[GA]^CG[CT]C
AflII 516 CTTAAG C^TTAAG
AlwI 425 GGATC GGATC(4/5)
Asp700I 353 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 997 CCCGGG C^[CT]CG[GA]G
AvaII 615 GGACC G^G[AT]CC
BanII 55 GGGCTC G[GA]GC[CT]^C
BbeI 151 GGCGCC GGCGC^C
BfrI 516 CTTAAG C^TTAAG
BmyI 55 GGGCTC G[AGT]GC[ACT]^C
BsaBI 546 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 151 GGCGCC G[GA]^CG[CT]C
BsgI 160 CTGCAC GTGCAG(16/14)
BsiYI 268 CCACCAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 268 CCACCAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 399 GAGAC GTCTC(1/5)
BsmI 215 GAATGC GAATGC(1/-1)
BsoBI 997 CCCGGG C^[CT]CG[GA]G
Bsp1286I 55 GGGCTC G[AGT]GC[ACT]^C
Bst1107I 846 GTATAC GTA^TAC
BstEII 510 GGTTACC G^GT[ACGT]ACC
BstNI 619 CCAGG CC^[AT]GG
BstPI 510 GGTTACC G^GT[ACGT]ACC
BstZ17I 846 GTATAC GTA^TAC
Cac8I 148 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 152 GCGC GCG^C
Cfr13I 615 GGACC G^G[ACGT]CC
DpnII 426 GATC ^GATC
EarI 480 CTCTTC CTCTTC(1/4)
EcoO65I 510 GGTTACC G^GT[ACGT]ACC
EcoRII 619 CCAGG ^CC[AT]GG
HaeII 151 GGCGCC [GA]GCGC^[CT]
HaeIII 239 GGCC GG^CC
HapII 998 CCGG C^CGG
HgaI 1090 GACGC GACGC(5/10)
HhaI 152 GCGC GCG^C
Hin1I 151 GGCGCC G[GA]^CG[CT]C
HinP1I 152 GCGC G^CGC
HpaII 998 CCGG C^CGG
HphI 407 TCACC GGTGA(8/7)
KasI 151 GGCGCC G^GCGCC
Ksp632I 480 CTCTTC CTCTTC(1/4)
MamI 546 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 426 GATC ^GATC
MslI 1198 CATCTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 366 CAGCTG C[AC]G^C[GT]G
MspI 998 CCGG C^CGG
MvaI 619 CCAGG CC^[AT]GG
MwoI 897 GCCAATCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 151 GGCGCC GG^CGCC
NdeII 426 GATC ^GATC
NspI 593 GCATGT [GA]CATG^[CT]
PshAI 616 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 251 CTGCAG CTGCA^G
PvuII 366 CAGCTG CAG^CTG
SapI 479 GCTCTTC GCTCTTC(1/4)
Sau3AI 426 GATC ^GATC
Sau96I 615 GGACC G^G[ACGT]CC
ScaI 1053 AGTACT AGT^ACT
SfcI 251 CTGCAG C^T[GA][CT]AG
SfoI 151 GGCGCC GGC^GCC
SmaI 997 CCCGGG CCC^GGG
SmlI 516 CTTAAG C^T[CT][GA]AG
TaqI 536 TCGA T^CGA
Tsp45I 193 GTCAC ^GT[GC]AC
TthHB8I 536 TCGA T^CGA
XmaCI 997 CCCGGG C^CCGGG
XmaI 997 CCCGGG C^CCGGG
XmnI 353 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 380 GTAC GT^AC
1054 GTAC
BcnI 997 CCCGG CC^[GC]GG
998 CCGGG
BfaI 903 CTAG C^TAG
971 CTAG
BpmI 396 CTGGAG CTGGAG(16/14)
555 CTGGAG
BsaJI 618 CCCAGG C^C[ACGT][ACGT]GG
997 CCCGGG
DraI 796 TTTAAA TTT^AAA
804 TTTAAA
MaeI 903 CTAG C^TAG
971 CTAG
NciI 997 CCCGG CC^[GC]GG
998 CCGGG
PleI 338 GAGTC GAGTC(4/5)
1187 GAGTC
RsaI 380 GTAC GT^AC
1054 GTAC
TfiI 49 GATTC G^A[AT]TC
371 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI ApoI BanI BbvI
BsrI BssKI BstF5I DdeI Fnu4HI
FokI HinfI ItaI MaeIII MboII
MnlI MseI NlaIII NlaIV ScrFI
Tru9I TseI Tsp509I TspRI