##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
AlwNI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HpaI KpnI KspI
MaeII MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 796 GGCGCC G[GA]^CG[CT]C
AhdI 298 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 428 AGCGCT AGC^GCT
ApaI 579 GGGCCC GGGCC^C
AspEI 298 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 53 CTCGGG C^[CT]CG[GA]G
BbeI 796 GGCGCC GGCGC^C
BbsI 711 GAAGAC GAAGAC(2/6)
BbvCI 720 GCTGAGG CCTCAGC(-5/-2)
BcnI 703 CCCGG CC^[GC]GG
BfaI 465 CTAG C^TAG
BglI 799 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1167 GCTTAGC GC^T[ACGT]AGC
BpmI 651 CTCCAG CTGGAG(16/14)
Bpu1102I 1167 GCTTAGC GC^T[ACGT]AGC
BpuAI 711 GAAGAC GAAGAC(2/6)
BsaHI 796 GGCGCC G[GA]^CG[CT]C
BsoBI 53 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1270 TGTACA T^GTACA
BsrDI 1259 GCAATG GCAATG(2/0)
BsrFI 613 GCCGGT [GA]^CCGG[CT]
BsrGI 1270 TGTACA T^GTACA
CelII 1167 GCTTAGC GC^T[ACGT]AGC
Cfr10I 613 GCCGGT [GA]^CCGG[CT]
DsaI 910 CCATGG C^C[GA][CT]GG
Eam1105I 298 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 428 AGCGCT AGC^GCT
EcoNI 716 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 369 GCGGG CCCGC(4/6)
Hin1I 796 GGCGCC G[GA]^CG[CT]C
HincII 1401 GTCAAC GT[CT]^[GA]AC
HindII 1401 GTCAAC GT[CT]^[GA]AC
KasI 796 GGCGCC G^GCGCC
MaeI 465 CTAG C^TAG
NarI 796 GGCGCC GG^CGCC
NciI 703 CCCGG CC^[GC]GG
NcoI 910 CCATGG C^CATGG
NdeI 1266 CATATG CA^TATG
NspI 786 GCATGT [GA]CATG^[CT]
PflMI 1174 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 338 CTGCAG CTGCA^G
SfoI 796 GGCGCC GGC^GCC
SspBI 1270 TGTACA T^GTACA
Van91I 1174 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 369 GCGG CCGC(-3/-1)
636 GCGG
AcsI 151 AAATTC [GA]^AATT[CT]
915 GAATTT
AlwI 674 GATCC GGATC(4/5)
1440 GATCC
ApoI 151 AAATTC [GA]^AATT[CT]
915 GAATTT
Asp700I 80 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
738 GAATATATTC
AspHI 239 GAGCAC G[AT]GC[AT]^C
1503 GTGCTC
AvaII 57 GGTCC G^G[AT]CC
629 GGACC
BalI 92 TGGCCA TGG^CCA
1073 TGGCCA
BanI 646 GGTGCC G^G[CT][GA]CC
796 GGCGCC
BanII 579 GGGCCC G[GA]GC[CT]^C
725 GGGCTC
BglII 553 AGATCT A^GATCT
658 AGATCT
BsaI 161 GAGACC GGTCTC(1/5)
190 GAGACC
BseRI 965 GAGGAG GAGGAG(10/8)
1446 GAGGAG
BsiHKAI 239 GAGCAC G[AT]GC[AT]^C
1503 GTGCTC
BstXI 210 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1124 CCATGACTGTGG
CfoI 429 GCGC GCG^C
797 GCGC
EaeI 92 TGGCCA [CT]^GGCC[GA]
1073 TGGCCA
EcoT14I 397 CCTTGG C^C[AT][AT]GG
910 CCATGG
HaeII 428 AGCGCT [GA]GCGC^[CT]
796 GGCGCC
HapII 614 CCGG C^CGG
704 CCGG
HhaI 429 GCGC GCG^C
797 GCGC
HinP1I 429 GCGC G^CGC
797 GCGC
HpaII 614 CCGG C^CGG
704 CCGG
MluNI 92 TGGCCA TGG^CCA
1073 TGGCCA
MscI 92 TGGCCA TGG^CCA
1073 TGGCCA
MslI 1055 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1125 CATGACTGTG
MspI 614 CCGG C^CGG
704 CCGG
SfaNI 393 GCATC GCATC(5/9)
1054 GCATC
SmlI 488 CTTGAG C^T[CT][GA]AG
1243 CTTGAG
StyI 397 CCTTGG C^C[AT][AT]GG
910 CCATGG
XcmI 210 CCAGCCCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
653 CCAGGAGATCTTTGG
XmnI 80 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
738 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AfaI AluI AocI BbvI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII