##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspI
BbeI BcgI BcnI BfrI BglI
BsaBI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FseI HaeII
HgaI HincII HindII HindIII HpaI
KasI KspI MamI MluI MroI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 108 GACGTC GACGT^C
Acc65I 919 GGTACC G^GTACC
AcsI 2575 AAATTC [GA]^AATT[CT]
AcyI 108 GACGTC G[GA]^CG[CT]C
AflIII 1323 ACATGT A^C[GA][CT]GT
AhdI 618 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2575 AAATTC [GA]^AATT[CT]
Asp718I 919 GGTACC G^GTACC
AspEI 618 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2413 TGCGCA TGC^GCA
AvrII 2333 CCTAGG C^CTAGG
BalI 2347 TGGCCA TGG^CCA
BbrPI 1647 CACGTG CAC^GTG
BbvCI 348 GCTGAGG CCTCAGC(-5/-2)
BciVI 29 GGATAC GTATCC(6/5)
BlnI 2333 CCTAGG C^CTAGG
BlpI 1038 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1038 GCTCAGC GC^T[ACGT]AGC
BsaAI 1647 CACGTG [CT]AC^GT[GA]
BsaHI 108 GACGTC G[GA]^CG[CT]C
BsmI 126 GAATGC GAATGC(1/-1)
BspLU11I 1323 ACATGT A^CATGT
BstAPI 836 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 930 GGTTACC G^GT[ACGT]ACC
BstPI 930 GGTTACC G^GT[ACGT]ACC
BstXI 1810 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1038 GCTCAGC GC^T[ACGT]AGC
DrdI 93 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2176 CCACGG C^C[GA][CT]GG
Eam1105I 618 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 930 GGTTACC G^GT[ACGT]ACC
FauI 689 GCGGG CCCGC(4/6)
FspI 2413 TGCGCA TGC^GCA
Hin1I 108 GACGTC G[GA]^CG[CT]C
KpnI 919 GGTACC GGTAC^C
MfeI 1375 CAATTG C^AATTG
MluNI 2347 TGGCCA TGG^CCA
MscI 2347 TGGCCA TGG^CCA
MunI 1375 CAATTG C^AATTG
NdeI 682 CATATG CA^TATG
PmaCI 1647 CACGTG CAC^GTG
PmlI 1647 CACGTG CAC^GTG
SacI 2262 GAGCTC GAGCT^C
SapI 186 GAAGAGC GCTCTTC(1/4)
SbfI 2231 CCTGCAGG CCTGCA^GG
ScaI 1987 AGTACT AGT^ACT
SexAI 926 ACCAGGT A^CC[AT]GGT
SmlI 1713 CTTGAG C^T[CT][GA]AG
SpeI 114 ACTAGT A^CTAGT
Sse8387I 2231 CCTGCAGG CCTGCA^GG
XbaI 600 TCTAGA T^CTAGA
XcmI 991 CCACACCTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 1623 CCTGAGG CC^T[ACGT]AGG
2118 CCTCAGG
AvaI 1615 CCCGAG C^[CT]CG[GA]G
2001 CCCGAG
BamHI 953 GGATCC G^GATCC
1673 GGATCC
BanI 919 GGTACC G^G[CT][GA]CC
1097 GGTGCC
BglII 476 AGATCT A^GATCT
1633 AGATCT
BsaWI 284 ACCGGA [AT]^CCGG[AT]
1888 ACCGGA
BsmFI 1418 GGGAC GGGAC(10/14)
197 GTCCC
BsoBI 1615 CCCGAG C^[CT]CG[GA]G
2001 CCCGAG
Bsp1407I 1163 TGTACA T^GTACA
1265 TGTACA
BsrBI 687 GAGCGG CCGCTC(-3/-3)
2139 GAGCGG
BsrDI 319 GCAATG GCAATG(2/0)
2017 GCAATG
BsrGI 1163 TGTACA T^GTACA
1265 TGTACA
Bsu36I 1623 CCTGAGG CC^T[ACGT]AGG
2118 CCTCAGG
DraII 145 AGGACCT [GA]G^G[ACGT]CC[CT]
2005 AGGACCT
Eco81I 1623 CCTGAGG CC^T[ACGT]AGG
2118 CCTCAGG
EcoO109I 145 AGGACCT [GA]G^G[ACGT]CC[CT]
2005 AGGACCT
HapII 285 CCGG C^CGG
1889 CCGG
HpaII 285 CCGG C^CGG
1889 CCGG
MaeII 109 ACGT A^CGT
1648 ACGT
MslI 20 CACGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2531 CATCTATGTG
MspA1I 219 CAGCTG C[AC]G^C[GT]G
2242 CAGCTG
MspI 285 CCGG C^CGG
1889 CCGG
PpuMI 145 AGGACCT [GA]G^G[AT]CC[CT]
2005 AGGACCT
PvuII 219 CAGCTG CAG^CTG
2242 CAGCTG
SspBI 1163 TGTACA T^GTACA
1265 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII