################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AccI AccII AccIII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspI BbeI BcgI BcnI BfrI BglI BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FseI HaeII HgaI HincII HindII HindIII HpaI KasI KspI MamI MluI MroI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 108 GACGTC GACGT^C Acc65I 919 GGTACC G^GTACC AcsI 2575 AAATTC [GA]^AATT[CT] AcyI 108 GACGTC G[GA]^CG[CT]C AflIII 1323 ACATGT A^C[GA][CT]GT AhdI 618 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 2575 AAATTC [GA]^AATT[CT] Asp718I 919 GGTACC G^GTACC AspEI 618 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2413 TGCGCA TGC^GCA AvrII 2333 CCTAGG C^CTAGG BalI 2347 TGGCCA TGG^CCA BbrPI 1647 CACGTG CAC^GTG BbvCI 348 GCTGAGG CCTCAGC(-5/-2) BciVI 29 GGATAC GTATCC(6/5) BlnI 2333 CCTAGG C^CTAGG BlpI 1038 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1038 GCTCAGC GC^T[ACGT]AGC BsaAI 1647 CACGTG [CT]AC^GT[GA] BsaHI 108 GACGTC G[GA]^CG[CT]C BsmI 126 GAATGC GAATGC(1/-1) BspLU11I 1323 ACATGT A^CATGT BstAPI 836 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 930 GGTTACC G^GT[ACGT]ACC BstPI 930 GGTTACC G^GT[ACGT]ACC BstXI 1810 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1038 GCTCAGC GC^T[ACGT]AGC DrdI 93 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2176 CCACGG C^C[GA][CT]GG Eam1105I 618 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 930 GGTTACC G^GT[ACGT]ACC FauI 689 GCGGG CCCGC(4/6) FspI 2413 TGCGCA TGC^GCA Hin1I 108 GACGTC G[GA]^CG[CT]C KpnI 919 GGTACC GGTAC^C MfeI 1375 CAATTG C^AATTG MluNI 2347 TGGCCA TGG^CCA MscI 2347 TGGCCA TGG^CCA MunI 1375 CAATTG C^AATTG NdeI 682 CATATG CA^TATG PmaCI 1647 CACGTG CAC^GTG PmlI 1647 CACGTG CAC^GTG SacI 2262 GAGCTC GAGCT^C SapI 186 GAAGAGC GCTCTTC(1/4) SbfI 2231 CCTGCAGG CCTGCA^GG ScaI 1987 AGTACT AGT^ACT SexAI 926 ACCAGGT A^CC[AT]GGT SmlI 1713 CTTGAG C^T[CT][GA]AG SpeI 114 ACTAGT A^CTAGT Sse8387I 2231 CCTGCAGG CCTGCA^GG XbaI 600 TCTAGA T^CTAGA XcmI 991 CCACACCTAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 1623 CCTGAGG CC^T[ACGT]AGG 2118 CCTCAGG AvaI 1615 CCCGAG C^[CT]CG[GA]G 2001 CCCGAG BamHI 953 GGATCC G^GATCC 1673 GGATCC BanI 919 GGTACC G^G[CT][GA]CC 1097 GGTGCC BglII 476 AGATCT A^GATCT 1633 AGATCT BsaWI 284 ACCGGA [AT]^CCGG[AT] 1888 ACCGGA BsmFI 1418 GGGAC GGGAC(10/14) 197 GTCCC BsoBI 1615 CCCGAG C^[CT]CG[GA]G 2001 CCCGAG Bsp1407I 1163 TGTACA T^GTACA 1265 TGTACA BsrBI 687 GAGCGG CCGCTC(-3/-3) 2139 GAGCGG BsrDI 319 GCAATG GCAATG(2/0) 2017 GCAATG BsrGI 1163 TGTACA T^GTACA 1265 TGTACA Bsu36I 1623 CCTGAGG CC^T[ACGT]AGG 2118 CCTCAGG DraII 145 AGGACCT [GA]G^G[ACGT]CC[CT] 2005 AGGACCT Eco81I 1623 CCTGAGG CC^T[ACGT]AGG 2118 CCTCAGG EcoO109I 145 AGGACCT [GA]G^G[ACGT]CC[CT] 2005 AGGACCT HapII 285 CCGG C^CGG 1889 CCGG HpaII 285 CCGG C^CGG 1889 CCGG MaeII 109 ACGT A^CGT 1648 ACGT MslI 20 CACGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2531 CATCTATGTG MspA1I 219 CAGCTG C[AC]G^C[GT]G 2242 CAGCTG MspI 285 CCGG C^CGG 1889 CCGG PpuMI 145 AGGACCT [GA]G^G[AT]CC[CT] 2005 AGGACCT PvuII 219 CAGCTG CAG^CTG 2242 CAGCTG SspBI 1163 TGTACA T^GTACA 1265 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII