##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HincII
HindII HpaI KpnI Ksp632I KspI
MamI MfeI MluI MroI MspA1I
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SexAI
SfiI SfuI SmaI SnaBI SpeI
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcsI 206 AAATTT [GA]^AATT[CT]
AcyI 635 GGCGCC G[GA]^CG[CT]C
AflIII 378 ACACGT A^C[GA][CT]GT
ApoI 206 AAATTT [GA]^AATT[CT]
AvaI 911 CCCGAG C^[CT]CG[GA]G
AvaII 1587 GGACC G^G[AT]CC
BbeI 635 GGCGCC GGCGC^C
BbsI 706 GAAGAC GAAGAC(2/6)
BcgI 71 GCACATGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 598 CCGGG CC^[GC]GG
BpuAI 706 GAAGAC GAAGAC(2/6)
BsaHI 635 GGCGCC G[GA]^CG[CT]C
BsaI 891 GAGACC GGTCTC(1/5)
BsmAI 891 GAGAC GTCTC(1/5)
BsmI 286 GCATTC GAATGC(1/-1)
BsoBI 911 CCCGAG C^[CT]CG[GA]G
BspHI 18 TCATGA T^CATGA
BspMI 1023 ACCTGC ACCTGC(4/8)
BsrFI 978 GCCGGC [GA]^CCGG[CT]
BstYI 1091 GGATCT [GA]^GATC[CT]
Cfr10I 978 GCCGGC [GA]^CCGG[CT]
DraI 257 TTTAAA TTT^AAA
DraII 1586 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 902 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1586 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1573 ATGCAT ATGCA^T
HaeII 635 GGCGCC [GA]GCGC^[CT]
Hin1I 635 GGCGCC G[GA]^CG[CT]C
HindIII 788 AAGCTT A^AGCTT
KasI 635 GGCGCC G^GCGCC
MflI 1091 GGATCT [GA]^GATC[CT]
MslI 1157 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 978 GCCGGC GCC^GGC
NarI 635 GGCGCC GG^CGCC
NciI 598 CCGGG CC^[GC]GG
NgoMIV 978 GCCGGC G^CCGGC
NsiI 1573 ATGCAT ATGCA^T
PpuMI 1586 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 18 TCATGA T^CATGA
SbfI 1199 CCTGCAGG CCTGCA^GG
ScaI 738 AGTACT AGT^ACT
SfoI 635 GGCGCC GGC^GCC
SgrAI 977 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1571 GCATGC GCATG^C
Sse8387I 1199 CCTGCAGG CCTGCA^GG
SspI 183 AATATT AAT^ATT
Tsp45I 1374 GTGAC ^GT[GC]AC
XhoII 1091 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 91 GATCC GGATC(4/5)
1091 GGATC
AlwNI 476 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1463 CAGACCCTG
BalI 645 TGGCCA TGG^CCA
733 TGGCCA
BfaI 449 CTAG C^TAG
1229 CTAG
BglI 638 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1133 GCCAGTTTGGC
BlpI 1015 GCTGAGC GC^T[ACGT]AGC
1190 GCTGAGC
Bpu1102I 1015 GCTGAGC GC^T[ACGT]AGC
1190 GCTGAGC
BsiYI 409 CCCAACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
675 CCAAGGACAGG
BslI 409 CCCAACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
675 CCAAGGACAGG
CelII 1015 GCTGAGC GC^T[ACGT]AGC
1190 GCTGAGC
EcoT14I 675 CCAAGG C^C[AT][AT]GG
1428 CCTTGG
HapII 598 CCGG C^CGG
979 CCGG
HgaI 82 GACGC GACGC(5/10)
1561 GACGC
HpaII 598 CCGG C^CGG
979 CCGG
MaeI 449 CTAG C^TAG
1229 CTAG
MaeII 380 ACGT A^CGT
710 ACGT
MaeIII 1225 GTTAC ^GT[ACGT]AC
1374 GTGAC
MluNI 645 TGGCCA TGG^CCA
733 TGGCCA
MscI 645 TGGCCA TGG^CCA
733 TGGCCA
MspI 598 CCGG C^CGG
979 CCGG
PleI 77 GACTC GAGTC(4/5)
995 GAGTC
StyI 675 CCAAGG C^C[AT][AT]GG
1428 CCTTGG
XcmI 622 CCACACACACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1128 CCATCGCCAGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BanI BanII BbvI BmyI BpmI
BsaJI BseRI BsiHKAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MboI MboII MnlI MseI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I