################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SexAI SfiI SfuI SmaI SnaBI SpeI SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 206 AAATTT [GA]^AATT[CT] AcyI 635 GGCGCC G[GA]^CG[CT]C AflIII 378 ACACGT A^C[GA][CT]GT ApoI 206 AAATTT [GA]^AATT[CT] AvaI 911 CCCGAG C^[CT]CG[GA]G AvaII 1587 GGACC G^G[AT]CC BbeI 635 GGCGCC GGCGC^C BbsI 706 GAAGAC GAAGAC(2/6) BcgI 71 GCACATGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 598 CCGGG CC^[GC]GG BpuAI 706 GAAGAC GAAGAC(2/6) BsaHI 635 GGCGCC G[GA]^CG[CT]C BsaI 891 GAGACC GGTCTC(1/5) BsmAI 891 GAGAC GTCTC(1/5) BsmI 286 GCATTC GAATGC(1/-1) BsoBI 911 CCCGAG C^[CT]CG[GA]G BspHI 18 TCATGA T^CATGA BspMI 1023 ACCTGC ACCTGC(4/8) BsrFI 978 GCCGGC [GA]^CCGG[CT] BstYI 1091 GGATCT [GA]^GATC[CT] Cfr10I 978 GCCGGC [GA]^CCGG[CT] DraI 257 TTTAAA TTT^AAA DraII 1586 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 902 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 1586 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT22I 1573 ATGCAT ATGCA^T HaeII 635 GGCGCC [GA]GCGC^[CT] Hin1I 635 GGCGCC G[GA]^CG[CT]C HindIII 788 AAGCTT A^AGCTT KasI 635 GGCGCC G^GCGCC MflI 1091 GGATCT [GA]^GATC[CT] MslI 1157 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 978 GCCGGC GCC^GGC NarI 635 GGCGCC GG^CGCC NciI 598 CCGGG CC^[GC]GG NgoMIV 978 GCCGGC G^CCGGC NsiI 1573 ATGCAT ATGCA^T PpuMI 1586 AGGACCT [GA]G^G[AT]CC[CT] RcaI 18 TCATGA T^CATGA SbfI 1199 CCTGCAGG CCTGCA^GG ScaI 738 AGTACT AGT^ACT SfoI 635 GGCGCC GGC^GCC SgrAI 977 CGCCGGCG C[GA]^CCGG[CT]G SphI 1571 GCATGC GCATG^C Sse8387I 1199 CCTGCAGG CCTGCA^GG SspI 183 AATATT AAT^ATT Tsp45I 1374 GTGAC ^GT[GC]AC XhoII 1091 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 91 GATCC GGATC(4/5) 1091 GGATC AlwNI 476 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 1463 CAGACCCTG BalI 645 TGGCCA TGG^CCA 733 TGGCCA BfaI 449 CTAG C^TAG 1229 CTAG BglI 638 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1133 GCCAGTTTGGC BlpI 1015 GCTGAGC GC^T[ACGT]AGC 1190 GCTGAGC Bpu1102I 1015 GCTGAGC GC^T[ACGT]AGC 1190 GCTGAGC BsiYI 409 CCCAACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 675 CCAAGGACAGG BslI 409 CCCAACGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 675 CCAAGGACAGG CelII 1015 GCTGAGC GC^T[ACGT]AGC 1190 GCTGAGC EcoT14I 675 CCAAGG C^C[AT][AT]GG 1428 CCTTGG HapII 598 CCGG C^CGG 979 CCGG HgaI 82 GACGC GACGC(5/10) 1561 GACGC HpaII 598 CCGG C^CGG 979 CCGG MaeI 449 CTAG C^TAG 1229 CTAG MaeII 380 ACGT A^CGT 710 ACGT MaeIII 1225 GTTAC ^GT[ACGT]AC 1374 GTGAC MluNI 645 TGGCCA TGG^CCA 733 TGGCCA MscI 645 TGGCCA TGG^CCA 733 TGGCCA MspI 598 CCGG C^CGG 979 CCGG PleI 77 GACTC GAGTC(4/5) 995 GAGTC StyI 675 CCAAGG C^C[AT][AT]GG 1428 CCTTGG XcmI 622 CCACACACACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1128 CCATCGCCAGTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AspHI BanI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MboI MboII MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I