##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI Aor51HI AscI AseI AsnI
Asp700I AspI AviII BbrPI BcgI
BclI BfrI BsaAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstUI BstXI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoT22I FbaI FspI
HincII HindII HindIII HpaI KspI
MluI MvnI NdeI NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2100 GGTACC G^GTACC
AccI 2843 GTAGAC GT^[AC][GT]AC
AcsI 2240 GAATTT [GA]^AATT[CT]
Alw44I 55 GTGCAC G^TGCAC
ApaLI 55 GTGCAC G^TGCAC
ApoI 2240 GAATTT [GA]^AATT[CT]
Asp718I 2100 GGTACC G^GTACC
AvrII 860 CCTAGG C^CTAGG
BalI 269 TGGCCA TGG^CCA
BbeI 1165 GGCGCC GGCGC^C
BbsI 2376 GTCTTC GAAGAC(2/6)
BbvCI 1997 CCTCAGC CCTCAGC(-5/-2)
BciVI 1083 GGATAC GTATCC(6/5)
BglII 1975 AGATCT A^GATCT
BlnI 860 CCTAGG C^CTAGG
BpuAI 2376 GTCTTC GAAGAC(2/6)
BsaBI 797 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1723 GAATGC GAATGC(1/-1)
BstBI 2379 TTCGAA TT^CGAA
DrdI 1741 GACTCCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 1821 GGCCGGCC GGCCGG^CC
KasI 1165 GGCGCC G^GCGCC
KpnI 2100 GGTACC GGTAC^C
MaeII 2181 ACGT A^CGT
MamI 797 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3197 CAATTG C^AATTG
MluNI 269 TGGCCA TGG^CCA
MscI 269 TGGCCA TGG^CCA
MunI 3197 CAATTG C^AATTG
NaeI 1822 GCCGGC GCC^GGC
NarI 1165 GGCGCC GG^CGCC
NgoMIV 1822 GCCGGC G^CCGGC
NspI 2910 GCATGT [GA]CATG^[CT]
NspV 2379 TTCGAA TT^CGAA
PflMI 2816 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 2258 GAGCTC GAGCT^C
SapI 3066 GAAGAGC GCTCTTC(1/4)
SfcI 2841 CTGTAG C^T[GA][CT]AG
SfoI 1165 GGCGCC GGC^GCC
SfuI 2379 TTCGAA TT^CGAA
SspI 2952 AATATT AAT^ATT
Van91I 2816 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 702 GGCGTC G[GA]^CG[CT]C
1165 GGCGCC
AhdI 830 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2628 GACAGCTGGTC
AlwNI 2451 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
2484 CAGCAGCTG
AspEI 830 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2628 GACAGCTGGTC
BsaHI 702 GGCGTC G[GA]^CG[CT]C
1165 GGCGCC
BsrFI 915 GCCGGT [GA]^CCGG[CT]
1822 GCCGGC
BstEII 495 GGTGACC G^GT[ACGT]ACC
1480 GGTTACC
BstPI 495 GGTGACC G^GT[ACGT]ACC
1480 GGTTACC
Cfr10I 915 GCCGGT [GA]^CCGG[CT]
1822 GCCGGC
DraI 3015 TTTAAA TTT^AAA
3290 TTTAAA
DraIII 94 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2167 CACAGGGTG
Eam1105I 830 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2628 GACAGCTGGTC
EcoNI 2668 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3125 CCTAGACTAGG
EcoO65I 495 GGTGACC G^GT[ACGT]ACC
1480 GGTTACC
EcoRV 784 GATATC GAT^ATC
1354 GATATC
HaeII 1165 GGCGCC [GA]GCGC^[CT]
1955 GGCGCT
HgaI 2326 GACGC GACGC(5/10)
703 GCGTC
Hin1I 702 GGCGTC G[GA]^CG[CT]C
1165 GGCGCC
MslI 309 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2300 CATGACGGTG
NcoI 572 CCATGG C^CATGG
2821 CCATGG
PaeR7I 148 CTCGAG C^TCGAG
193 CTCGAG
XhoI 148 CTCGAG C^TCGAG
193 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MroI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfiI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI