##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI BamHI BbeI BfrI BglII
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BssSI BstBI BstEII BstPI
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI HaeII HincII HindII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 445 GGTACC G^GTACC
AccII 1971 CGCG CG^CG
AflIII 3324 ACATGT A^C[GA][CT]GT
Asp700I 1539 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 445 GGTACC G^GTACC
AviII 2202 TGCGCA TGC^GCA
BbvCI 2398 GCTGAGG CCTCAGC(-5/-2)
BcgI 251 GCAGAGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI 2765 CGTCTC CGTCTC(1/5)
Bsp1407I 780 TGTACA T^GTACA
BspHI 1140 TCATGA T^CATGA
BspLU11I 3324 ACATGT A^CATGT
BsrFI 3600 GCCGGC [GA]^CCGG[CT]
BsrGI 780 TGTACA T^GTACA
Bst1107I 1636 GTATAC GTA^TAC
BstAPI 830 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1971 CGCG CG^CG
BstYI 3574 GGATCT [GA]^GATC[CT]
BstZ17I 1636 GTATAC GTA^TAC
Cfr10I 3600 GCCGGC [GA]^CCGG[CT]
DraI 4319 TTTAAA TTT^AAA
EcoRI 2213 GAATTC G^AATTC
EcoT22I 3994 ATGCAT ATGCA^T
FspI 2202 TGCGCA TGC^GCA
KpnI 445 GGTACC GGTAC^C
MflI 3574 GGATCT [GA]^GATC[CT]
MvnI 1971 CGCG CG^CG
NaeI 3600 GCCGGC GCC^GGC
NdeI 1845 CATATG CA^TATG
NgoMIV 3600 GCCGGC G^CCGGC
NheI 2536 GCTAGC G^CTAGC
NsiI 3994 ATGCAT ATGCA^T
RcaI 1140 TCATGA T^CATGA
SapI 4079 GAAGAGC GCTCTTC(1/4)
SbfI 2881 CCTGCAGG CCTGCA^GG
SmaI 4096 CCCGGG CCC^GGG
Sse8387I 2881 CCTGCAGG CCTGCA^GG
SspBI 780 TGTACA T^GTACA
SspI 1650 AATATT AAT^ATT
TaqI 1174 TCGA T^CGA
TthHB8I 1174 TCGA T^CGA
XhoII 3574 GGATCT [GA]^GATC[CT]
XmaCI 4096 CCCGGG C^CCGGG
XmaI 4096 CCCGGG C^CCGGG
XmnI 1539 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1636 GTATAC GT^[AC][GT]AC
3271 GTCTAC
AcyI 797 GACGCC G[GA]^CG[CT]C
3077 GGCGTC
Alw44I 2594 GTGCAC G^TGCAC
3480 GTGCAC
AocI 2185 CCTTAGG CC^T[ACGT]AGG
3202 CCTGAGG
ApaI 1286 GGGCCC GGGCC^C
2191 GGGCCC
ApaLI 2594 GTGCAC G^TGCAC
3480 GTGCAC
AvrII 2939 CCTAGG C^CTAGG
3784 CCTAGG
BbsI 4189 GAAGAC GAAGAC(2/6)
2438 GTCTTC
BciVI 904 GTATCC GTATCC(6/5)
3789 GTATCC
BclI 689 TGATCA T^GATCA
1032 TGATCA
BlnI 2939 CCTAGG C^CTAGG
3784 CCTAGG
BpuAI 4189 GAAGAC GAAGAC(2/6)
2438 GTCTTC
BsaHI 797 GACGCC G[GA]^CG[CT]C
3077 GGCGTC
Bsu36I 2185 CCTTAGG CC^T[ACGT]AGG
3202 CCTGAGG
DrdI 3363 GACATTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4223 GACCTTGAGGTC
EarI 307 GAAGAG CTCTTC(1/4)
4079 GAAGAG
Eco81I 2185 CCTTAGG CC^T[ACGT]AGG
3202 CCTGAGG
EcoNI 3198 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3656 CCTGGTTCAGG
FauI 2671 GCGGG CCCGC(4/6)
2801 GCGGG
FbaI 689 TGATCA T^GATCA
1032 TGATCA
Hin1I 797 GACGCC G[GA]^CG[CT]C
3077 GGCGTC
HindIII 1427 AAGCTT A^AGCTT
3387 AAGCTT
Ksp632I 307 GAAGAG CTCTTC(1/4)
4079 GAAGAG
PflMI 414 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4245 CCAAAATATGG
PvuII 2909 CAGCTG CAG^CTG
3605 CAGCTG
SacI 256 GAGCTC GAGCT^C
3527 GAGCTC
SphI 227 GCATGC GCATG^C
2199 GCATGC
StuI 236 AGGCCT AGG^CCT
959 AGGCCT
Van91I 414 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4245 CCAAAATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PmaCI PmlI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XcmI