##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvrII BamHI BbeI BbrPI BcgI
BciVI BfrI BglII BlnI BsaBI
BsaHI BsiEI BsiWI BspDI BspHI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspI SwaI Van91I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 1144 CGCG CG^CG
AccIII 1754 TCCGGA T^CCGGA
AflIII 499 ACGTGT A^C[GA][CT]GT
AseI 2922 ATTAAT AT^TAAT
AsnI 2922 ATTAAT AT^TAAT
AspI 852 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 839 CCCGGG C^[CT]CG[GA]G
AviII 2203 TGCGCA TGC^GCA
BanI 3161 GGTGCC G^G[CT][GA]CC
BclI 2345 TGATCA T^GATCA
BsaAI 498 TACGTG [CT]AC^GT[GA]
BsaWI 1754 TCCGGA [AT]^CCGG[AT]
BseAI 1754 TCCGGA T^CCGGA
BsmBI 1304 GAGACG CGTCTC(1/5)
BsmI 565 GAATGC GAATGC(1/-1)
BsoBI 839 CCCGGG C^[CT]CG[GA]G
Bsp1407I 732 TGTACA T^GTACA
BspEI 1754 TCCGGA T^CCGGA
BsrGI 732 TGTACA T^GTACA
BssSI 2037 CACGAG CACGAG(-5/-1)
BstUI 1144 CGCG CG^CG
DraII 1651 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1651 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 2345 TGATCA T^GATCA
FspI 2203 TGCGCA TGC^GCA
HgaI 437 GACGC GACGC(5/10)
MaeII 499 ACGT A^CGT
MroI 1754 TCCGGA T^CCGGA
MvnI 1144 CGCG CG^CG
NdeI 3262 CATATG CA^TATG
PflFI 852 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1651 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1849 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2922 ATTAAT AT^TAAT
SapI 2832 GCTCTTC GCTCTTC(1/4)
SexAI 3157 ACCTGGT A^CC[AT]GGT
SfiI 1104 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 839 CCCGGG CCC^GGG
SmlI 1443 CTTGAG C^T[CT][GA]AG
SpeI 2784 ACTAGT A^CTAGT
SspBI 732 TGTACA T^GTACA
Tth111I 852 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2997 TCTAGA T^CTAGA
XmaCI 839 CCCGGG C^CCGGG
XmaI 839 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 756 CCTGAGG CC^T[ACGT]AGG
1983 CCTAAGG
Asp700I 19 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2156 GAAAATCTTC
AvaII 430 GGACC G^G[AT]CC
1652 GGACC
BalI 190 TGGCCA TGG^CCA
2546 TGGCCA
BglI 1105 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2539 GCCTCCATGGC
BlpI 470 GCTTAGC GC^T[ACGT]AGC
2136 GCTCAGC
Bpu1102I 470 GCTTAGC GC^T[ACGT]AGC
2136 GCTCAGC
BsaI 535 GAGACC GGTCTC(1/5)
2649 GGTCTC
BsrDI 2888 CATTGC GCAATG(2/0)
2938 CATTGC
BstXI 606 CCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
643 CCAAATTGCTGG
BstYI 1022 AGATCC [GA]^GATC[CT]
3369 AGATCC
Bsu36I 756 CCTGAGG CC^T[ACGT]AGG
1983 CCTAAGG
CelII 470 GCTTAGC GC^T[ACGT]AGC
2136 GCTCAGC
CfoI 951 GCGC GCG^C
2204 GCGC
Eco81I 756 CCTGAGG CC^T[ACGT]AGG
1983 CCTAAGG
HhaI 951 GCGC GCG^C
2204 GCGC
HinP1I 951 GCGC G^CGC
2204 GCGC
MflI 1022 AGATCC [GA]^GATC[CT]
3369 AGATCC
MluNI 190 TGGCCA TGG^CCA
2546 TGGCCA
MscI 190 TGGCCA TGG^CCA
2546 TGGCCA
NcoI 956 CCATGG C^CATGG
2543 CCATGG
SacI 1069 GAGCTC GAGCT^C
1933 GAGCTC
TfiI 2552 GATTC G^A[AT]TC
3062 GAATC
XhoII 1022 AGATCC [GA]^GATC[CT]
3369 AGATCC
XmnI 19 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2156 GAAAATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I