##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccI AgeI AhdI Alw44I ApaI
ApaLI AscI AspEI AspI AvrII
BalI BbeI BbrPI BlnI BsaAI
BsiEI BsiWI BsmFI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoRV FseI
HgaI HindIII HpaI KasI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 210 GACGTC GACGT^C
AccII 1222 CGCG CG^CG
AccIII 3282 TCCGGA T^CCGGA
AclI 3764 AACGTT AA^CGTT
AcyI 210 GACGTC G[GA]^CG[CT]C
AflII 1180 CTTAAG C^TTAAG
Aor51HI 3657 AGCGCT AGC^GCT
AseI 1291 ATTAAT AT^TAAT
AsnI 1291 ATTAAT AT^TAAT
BbvCI 3045 CCTCAGC CCTCAGC(-5/-2)
BcgI 1476 GCATTTTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1524 GGATAC GTATCC(6/5)
BcnI 2450 CCGGG CC^[GC]GG
BfrI 1180 CTTAAG C^TTAAG
BglI 1837 GCCTGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 210 GACGTC G[GA]^CG[CT]C
BseAI 3282 TCCGGA T^CCGGA
BsmBI 777 GAGACG CGTCTC(1/5)
BspEI 3282 TCCGGA T^CCGGA
BspLU11I 1298 ACATGT A^CATGT
BsrBI 2766 CCGCTC CCGCTC(-3/-3)
BsrDI 2760 CATTGC GCAATG(2/0)
BsrFI 2836 GCCGGT [GA]^CCGG[CT]
BssSI 2676 CTCGTG CACGAG(-5/-1)
BstAPI 1153 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1222 CGCG CG^CG
Cfr10I 2836 GCCGGT [GA]^CCGG[CT]
EaeI 1801 CGGCCA [CT]^GGCC[GA]
Eco47III 3657 AGCGCT AGC^GCT
EcoNI 1443 CCTGTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1492 ATGCAT ATGCA^T
HaeII 3657 AGCGCT [GA]GCGC^[CT]
Hin1I 210 GACGTC G[GA]^CG[CT]C
HincII 867 GTCAAC GT[CT]^[GA]AC
HindII 867 GTCAAC GT[CT]^[GA]AC
MroI 3282 TCCGGA T^CCGGA
MslI 1533 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1222 CGCG CG^CG
NciI 2450 CCGGG CC^[GC]GG
NheI 372 GCTAGC G^CTAGC
NsiI 1492 ATGCAT ATGCA^T
PaeR7I 1909 CTCGAG C^TCGAG
PflMI 57 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1291 ATTAAT AT^TAAT
Psp1406I 3764 AACGTT AA^CGTT
ScaI 50 AGTACT AGT^ACT
SexAI 2009 ACCAGGT A^CC[AT]GGT
StuI 414 AGGCCT AGG^CCT
Van91I 57 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 999 TCTAGA T^CTAGA
XcmI 3171 CCAGTACCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1909 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2453 GGTACC G^GTACC
2482 GGTACC
AflIII 1298 ACATGT A^C[GA][CT]GT
2544 ACGTGT
AocI 417 CCTCAGG CC^T[ACGT]AGG
3157 CCTGAGG
Asp718I 2453 GGTACC G^GTACC
2482 GGTACC
AvaI 1909 CTCGAG C^[CT]CG[GA]G
2719 CCCGAG
AviII 1559 TGCGCA TGC^GCA
2279 TGCGCA
BamHI 3162 GGATCC G^GATCC
3641 GGATCC
BbsI 127 GAAGAC GAAGAC(2/6)
3698 GAAGAC
BlpI 367 GCTCAGC GC^T[ACGT]AGC
2354 GCTTAGC
Bpu1102I 367 GCTCAGC GC^T[ACGT]AGC
2354 GCTTAGC
BpuAI 127 GAAGAC GAAGAC(2/6)
3698 GAAGAC
BsaBI 1929 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3439 GATGGCAATC
BsaWI 897 ACCGGA [AT]^CCGG[AT]
3282 TCCGGA
BseRI 2769 CTCCTC GAGGAG(10/8)
1066 GAGGAG
BsgI 2679 GTGCAG GTGCAG(16/14)
3429 GTGCAG
BsoBI 1909 CTCGAG C^[CT]CG[GA]G
2719 CCCGAG
BstEII 3597 GGTGACC G^GT[ACGT]ACC
3625 GGTAACC
BstPI 3597 GGTGACC G^GT[ACGT]ACC
3625 GGTAACC
BstXI 2401 CCACAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3005 CCATCCGCTTGG
Bsu36I 417 CCTCAGG CC^T[ACGT]AGG
3157 CCTGAGG
CelII 367 GCTCAGC GC^T[ACGT]AGC
2354 GCTTAGC
DsaI 1823 CCATGG C^C[GA][CT]GG
2018 CCATGG
Eco81I 417 CCTCAGG CC^T[ACGT]AGG
3157 CCTGAGG
EcoO65I 3597 GGTGACC G^GT[ACGT]ACC
3625 GGTAACC
EcoRI 1583 GAATTC G^AATTC
2913 GAATTC
FauI 2752 CCCGC CCCGC(4/6)
2765 CCCGC
FspI 1559 TGCGCA TGC^GCA
2279 TGCGCA
KpnI 2453 GGTACC GGTAC^C
2482 GGTACC
MamI 1929 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3439 GATGGCAATC
NcoI 1823 CCATGG C^CATGG
2018 CCATGG
NdeI 2243 CATATG CA^TATG
3527 CATATG
NspI 1298 ACATGT [GA]CATG^[CT]
1553 ACATGC
SspI 1105 AATATT AAT^ATT
2970 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbvI BclI BfaI
BglII BmyI BpmI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI