##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AgeI ApoI AscI AspI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1695 GGTACC G^GTACC
AflIII 2752 ACATGT A^C[GA][CT]GT
AhdI 1659 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2441 GTGCAC G^TGCAC
Aor51HI 2430 AGCGCT AGC^GCT
ApaLI 2441 GTGCAC G^TGCAC
AseI 2037 ATTAAT AT^TAAT
AsnI 2037 ATTAAT AT^TAAT
Asp718I 1695 GGTACC G^GTACC
AspEI 1659 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 382 TGCGCA TGC^GCA
BcnI 2413 CCGGG CC^[GC]GG
BlpI 2911 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2911 GCTCAGC GC^T[ACGT]AGC
BsaBI 2207 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2378 GAGACC GGTCTC(1/5)
BsmBI 2871 GAGACG CGTCTC(1/5)
BspLU11I 2752 ACATGT A^CATGT
BsrBI 161 CCGCTC CCGCTC(-3/-3)
BsrFI 1969 ACCGGC [GA]^CCGG[CT]
BstXI 1700 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2911 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1969 ACCGGC [GA]^CCGG[CT]
DraIII 1554 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1659 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2430 AGCGCT AGC^GCT
FspI 382 TGCGCA TGC^GCA
KpnI 1695 GGTACC GGTAC^C
MaeII 2874 ACGT A^CGT
MaeIII 2301 GTTAC ^GT[ACGT]AC
MamI 2207 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 2413 CCGGG CC^[GC]GG
NdeI 1456 CATATG CA^TATG
PshBI 2037 ATTAAT AT^TAAT
SapI 846 GCTCTTC GCTCTTC(1/4)
SphI 2521 GCATGC GCATG^C
XcmI 455 CCACTTTTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 2275 GTCTAC GT^[AC][GT]AC
3007 GTCTAC
AccII 2298 CGCG CG^CG
2605 CGCG
Asp700I 529 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2711 GAAAAACTTC
AspHI 1873 GAGCAC G[AT]GC[AT]^C
2441 GTGCAC
BalI 2086 TGGCCA TGG^CCA
2805 TGGCCA
BamHI 1033 GGATCC G^GATCC
3280 GGATCC
BsiHKAI 1873 GAGCAC G[AT]GC[AT]^C
2441 GTGCAC
BstUI 2298 CGCG CG^CG
2605 CGCG
EcoNI 2465 CCTGAGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2836 CCTTCCTCAGG
FauI 331 CCCGC CCCGC(4/6)
1948 CCCGC
HaeII 2430 AGCGCT [GA]GCGC^[CT]
2772 AGCGCC
MluNI 2086 TGGCCA TGG^CCA
2805 TGGCCA
MscI 2086 TGGCCA TGG^CCA
2805 TGGCCA
MseI 2038 TTAA T^TAA
2264 TTAA
MvnI 2298 CGCG CG^CG
2605 CGCG
TaqI 1514 TCGA T^CGA
1931 TCGA
Tru9I 2038 TTAA T^TAA
2264 TTAA
TthHB8I 1514 TCGA T^CGA
1931 TCGA
XmnI 529 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2711 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AvaI AvaII AvrII
BanI BanII BbsI BbvCI BbvI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI TseI
Tsp509I TspRI Van91I XhoII