##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BcnI BlnI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV FauI FseI
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI MamI MfeI
MluNI MroI MscI MspI MunI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 3151 ACGCGT A^C[GA][CT]GT
AseI 517 ATTAAT AT^TAAT
AsnI 517 ATTAAT AT^TAAT
Asp700I 3493 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2811 GGATCC G^GATCC
BclI 964 TGATCA T^GATCA
BglI 69 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1283 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1283 GCTGAGC GC^T[ACGT]AGC
BseRI 1837 GAGGAG GAGGAG(10/8)
BsmBI 3262 GAGACG CGTCTC(1/5)
BstEII 1163 GGTAACC G^GT[ACGT]ACC
BstPI 1163 GGTAACC G^GT[ACGT]ACC
CelII 1283 GCTGAGC GC^T[ACGT]AGC
DsaI 2076 CCGCGG C^C[GA][CT]GG
EcoO65I 1163 GGTAACC G^GT[ACGT]ACC
EcoT22I 901 ATGCAT ATGCA^T
FbaI 964 TGATCA T^GATCA
HaeII 3119 GGCGCT [GA]GCGC^[CT]
HincII 882 GTTGAC GT[CT]^[GA]AC
HindII 882 GTTGAC GT[CT]^[GA]AC
KspI 2076 CCGCGG CCGC^GG
MluI 3151 ACGCGT A^CGCGT
NheI 3413 GCTAGC G^CTAGC
NsiI 901 ATGCAT ATGCA^T
PflMI 2699 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1943 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 517 ATTAAT AT^TAAT
SacI 3222 GAGCTC GAGCT^C
SacII 2076 CCGCGG CCGC^GG
SapI 1742 GCTCTTC GCTCTTC(1/4)
SnaBI 2557 TACGTA TAC^GTA
SphI 2671 GCATGC GCATG^C
SspI 3496 AATATT AAT^ATT
StuI 2725 AGGCCT AGG^CCT
Van91I 2699 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1812 CCAGAGTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3493 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 2077 CGCG CG^CG
3152 CGCG
AflII 1000 CTTAAG C^TTAAG
2264 CTTAAG
AocI 1387 CCTCAGG CC^T[ACGT]AGG
1729 CCTCAGG
AvaII 1944 GGACC G^G[AT]CC
2133 GGTCC
AviII 810 TGCGCA TGC^GCA
3231 TGCGCA
BbsI 1463 GAAGAC GAAGAC(2/6)
1714 GTCTTC
BciVI 3165 GGATAC GTATCC(6/5)
3249 GGATAC
BfrI 1000 CTTAAG C^TTAAG
2264 CTTAAG
BglII 1019 AGATCT A^GATCT
1797 AGATCT
BpuAI 1463 GAAGAC GAAGAC(2/6)
1714 GTCTTC
BsaAI 636 CACGTA [CT]AC^GT[GA]
2557 TACGTA
BsaI 2056 GAGACC GGTCTC(1/5)
2956 GAGACC
BsmI 588 GAATGC GAATGC(1/-1)
735 GAATGC
BspHI 213 TCATGA T^CATGA
731 TCATGA
BspMI 2059 ACCTGC ACCTGC(4/8)
3409 ACCTGC
BstUI 2077 CGCG CG^CG
3152 CGCG
Bsu36I 1387 CCTCAGG CC^T[ACGT]AGG
1729 CCTCAGG
Eco81I 1387 CCTCAGG CC^T[ACGT]AGG
1729 CCTCAGG
EcoRI 209 GAATTC G^AATTC
1914 GAATTC
EcoT14I 1168 CCTTGG C^C[AT][AT]GG
3135 CCTTGG
FspI 810 TGCGCA TGC^GCA
3231 TGCGCA
HphI 2869 GGTGA GGTGA(8/7)
2138 TCACC
MvnI 2077 CGCG CG^CG
3152 CGCG
NdeI 904 CATATG CA^TATG
935 CATATG
NspI 899 ACATGC [GA]CATG^[CT]
2671 GCATGC
PstI 1877 CTGCAG CTGCA^G
3381 CTGCAG
PvuII 1595 CAGCTG CAG^CTG
1820 CAGCTG
RcaI 213 TCATGA T^CATGA
731 TCATGA
SpeI 2222 ACTAGT A^CTAGT
3141 ACTAGT
StyI 1168 CCTTGG C^C[AT][AT]GG
3135 CCTTGG
TaqI 1927 TCGA T^CGA
3365 TCGA
TthHB8I 1927 TCGA T^CGA
3365 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
BanII BbvI BfaI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII