##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
BbrPI BclI BfrI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspEI BsrDI
BsrFI BssHII BstBI BstEII BstPI
BstUI CelII Cfr10I CpoI DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HgaI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI Tth111I Van91I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 2444 GGCGCC G[GA]^CG[CT]C
Aor51HI 87 AGCGCT AGC^GCT
ApaI 1731 GGGCCC GGGCC^C
AvrII 926 CCTAGG C^CTAGG
BamHI 1826 GGATCC G^GATCC
BbeI 2444 GGCGCC GGCGC^C
BbvCI 2320 CCTCAGC CCTCAGC(-5/-2)
BcgI 1701 GCAGTGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 149 GTATCC GTATCC(6/5)
BlnI 926 CCTAGG C^CTAGG
BsaHI 2444 GGCGCC G[GA]^CG[CT]C
BsaWI 621 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3341 TGTACA T^GTACA
BspDI 1709 ATCGAT AT^CGAT
BspLU11I 3130 ACATGT A^CATGT
BsrBI 1220 CCGCTC CCGCTC(-3/-3)
BsrGI 3341 TGTACA T^GTACA
BssSI 848 CACGAG CACGAG(-5/-1)
Bst1107I 3361 GTATAC GTA^TAC
BstAPI 1648 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3361 GTATAC GTA^TAC
ClaI 1709 ATCGAT AT^CGAT
DraI 856 TTTAAA TTT^AAA
DraIII 2311 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 87 AGCGCT AGC^GCT
EcoNI 976 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2835 ATGCAT ATGCA^T
Hin1I 2444 GGCGCC G[GA]^CG[CT]C
HincII 1969 GTTGAC GT[CT]^[GA]AC
HindII 1969 GTTGAC GT[CT]^[GA]AC
KasI 2444 GGCGCC G^GCGCC
NarI 2444 GGCGCC GG^CGCC
NheI 121 GCTAGC G^CTAGC
NsiI 2835 ATGCAT ATGCA^T
PshAI 898 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1490 CCTGCAGG CCTGCA^GG
SfoI 2444 GGCGCC GGC^GCC
Sse8387I 1490 CCTGCAGG CCTGCA^GG
SspBI 3341 TGTACA T^GTACA
TaqI 1710 TCGA T^CGA
TthHB8I 1710 TCGA T^CGA
XbaI 2270 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 21 GTCTAC GT^[AC][GT]AC
3361 GTATAC
AflIII 2521 ACGTGT A^C[GA][CT]GT
3130 ACATGT
AhdI 302 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
708 GACTGGTGGTC
AocI 1952 CCTAAGG CC^T[ACGT]AGG
2073 CCTGAGG
AspEI 302 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
708 GACTGGTGGTC
BbsI 920 GTCTTC GAAGAC(2/6)
1445 GTCTTC
BglII 108 AGATCT A^GATCT
2701 AGATCT
BpuAI 920 GTCTTC GAAGAC(2/6)
1445 GTCTTC
BspHI 42 TCATGA T^CATGA
1119 TCATGA
Bsu36I 1952 CCTAAGG CC^T[ACGT]AGG
2073 CCTGAGG
Eam1105I 302 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
708 GACTGGTGGTC
Eco81I 1952 CCTAAGG CC^T[ACGT]AGG
2073 CCTGAGG
MaeII 2521 ACGT A^CGT
2710 ACGT
NspI 1074 ACATGC [GA]CATG^[CT]
3130 ACATGT
RcaI 42 TCATGA T^CATGA
1119 TCATGA
SacI 64 GAGCTC GAGCT^C
1906 GAGCTC
SapI 1207 GCTCTTC GCTCTTC(1/4)
1222 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII