################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII BbrPI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspEI BsrDI BsrFI BssHII BstBI BstEII BstPI BstUI CelII Cfr10I CpoI DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 2444 GGCGCC G[GA]^CG[CT]C Aor51HI 87 AGCGCT AGC^GCT ApaI 1731 GGGCCC GGGCC^C AvrII 926 CCTAGG C^CTAGG BamHI 1826 GGATCC G^GATCC BbeI 2444 GGCGCC GGCGC^C BbvCI 2320 CCTCAGC CCTCAGC(-5/-2) BcgI 1701 GCAGTGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 149 GTATCC GTATCC(6/5) BlnI 926 CCTAGG C^CTAGG BsaHI 2444 GGCGCC G[GA]^CG[CT]C BsaWI 621 ACCGGA [AT]^CCGG[AT] Bsp1407I 3341 TGTACA T^GTACA BspDI 1709 ATCGAT AT^CGAT BspLU11I 3130 ACATGT A^CATGT BsrBI 1220 CCGCTC CCGCTC(-3/-3) BsrGI 3341 TGTACA T^GTACA BssSI 848 CACGAG CACGAG(-5/-1) Bst1107I 3361 GTATAC GTA^TAC BstAPI 1648 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 3361 GTATAC GTA^TAC ClaI 1709 ATCGAT AT^CGAT DraI 856 TTTAAA TTT^AAA DraIII 2311 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 87 AGCGCT AGC^GCT EcoNI 976 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2835 ATGCAT ATGCA^T Hin1I 2444 GGCGCC G[GA]^CG[CT]C HincII 1969 GTTGAC GT[CT]^[GA]AC HindII 1969 GTTGAC GT[CT]^[GA]AC KasI 2444 GGCGCC G^GCGCC NarI 2444 GGCGCC GG^CGCC NheI 121 GCTAGC G^CTAGC NsiI 2835 ATGCAT ATGCA^T PshAI 898 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 1490 CCTGCAGG CCTGCA^GG SfoI 2444 GGCGCC GGC^GCC Sse8387I 1490 CCTGCAGG CCTGCA^GG SspBI 3341 TGTACA T^GTACA TaqI 1710 TCGA T^CGA TthHB8I 1710 TCGA T^CGA XbaI 2270 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 21 GTCTAC GT^[AC][GT]AC 3361 GTATAC AflIII 2521 ACGTGT A^C[GA][CT]GT 3130 ACATGT AhdI 302 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 708 GACTGGTGGTC AocI 1952 CCTAAGG CC^T[ACGT]AGG 2073 CCTGAGG AspEI 302 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 708 GACTGGTGGTC BbsI 920 GTCTTC GAAGAC(2/6) 1445 GTCTTC BglII 108 AGATCT A^GATCT 2701 AGATCT BpuAI 920 GTCTTC GAAGAC(2/6) 1445 GTCTTC BspHI 42 TCATGA T^CATGA 1119 TCATGA Bsu36I 1952 CCTAAGG CC^T[ACGT]AGG 2073 CCTGAGG Eam1105I 302 GACAATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 708 GACTGGTGGTC Eco81I 1952 CCTAAGG CC^T[ACGT]AGG 2073 CCTGAGG MaeII 2521 ACGT A^CGT 2710 ACGT NspI 1074 ACATGC [GA]CATG^[CT] 3130 ACATGT RcaI 42 TCATGA T^CATGA 1119 TCATGA SacI 64 GAGCTC GAGCT^C 1906 GAGCTC SapI 1207 GCTCTTC GCTCTTC(1/4) 1222 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII