##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AflII AscI AseI AsnI
Asp700I AviII BamHI BbeI BbrPI
BciVI BclI BfrI BglII BsaAI
BsiWI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI EcoO65I EcoRV EcoT22I
FbaI FspI KasI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 193 GACGTC GACGT^C
AccI 1792 GTCTAC GT^[AC][GT]AC
AccII 671 CGCG CG^CG
AcyI 193 GACGTC G[GA]^CG[CT]C
AflIII 924 ACATGT A^C[GA][CT]GT
AlwI 355 GATCC GGATC(4/5)
Aor51HI 129 AGCGCT AGC^GCT
AspI 695 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2110 CCTAGG C^CTAGG
BbsI 2361 GAAGAC GAAGAC(2/6)
BbvCI 1469 CCTCAGC CCTCAGC(-5/-2)
BlnI 2110 CCTAGG C^CTAGG
BlpI 889 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 889 GCTCAGC GC^T[ACGT]AGC
BpuAI 2361 GAAGAC GAAGAC(2/6)
BsaBI 790 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 193 GACGTC G[GA]^CG[CT]C
BsmBI 191 GAGACG CGTCTC(1/5)
BsmI 2521 GAATGC GAATGC(1/-1)
BspHI 1718 TCATGA T^CATGA
BspLU11I 924 ACATGT A^CATGT
BsrBI 41 GAGCGG CCGCTC(-3/-3)
BstAPI 1300 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 671 CGCG CG^CG
BstXI 478 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 354 AGATCC [GA]^GATC[CT]
CelII 889 GCTCAGC GC^T[ACGT]AGC
CpoI 1383 CGGTCCG CG^G[AT]CCG
DraI 3173 TTTAAA TTT^AAA
DrdI 1843 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 484 CCATGG C^C[GA][CT]GG
Eco47III 129 AGCGCT AGC^GCT
EcoRI 1686 GAATTC G^AATTC
FseI 896 GGCCGGCC GGCCGG^CC
HgaI 1403 GACGC GACGC(5/10)
Hin1I 193 GACGTC G[GA]^CG[CT]C
HindIII 1947 AAGCTT A^AGCTT
HpaI 2914 GTTAAC GTT^AAC
MamI 790 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 354 AGATCC [GA]^GATC[CT]
MslI 479 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 671 CGCG CG^CG
NaeI 897 GCCGGC GCC^GGC
NcoI 484 CCATGG C^CATGG
NgoMIV 897 GCCGGC G^CCGGC
PflFI 695 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1807 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 676 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1819 CAGCTG CAG^CTG
RcaI 1718 TCATGA T^CATGA
RsrII 1383 CGGTCCG CG^G[AT]CCG
SacI 998 GAGCTC GAGCT^C
ScaI 435 AGTACT AGT^ACT
SfiI 2040 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1374 GCATGC GCATG^C
TaqI 519 TCGA T^CGA
Tth111I 695 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 519 TCGA T^CGA
Van91I 1807 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1690 TCTAGA T^CTAGA
XhoII 354 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1686 GAATTC [GA]^AATT[CT]
2474 AAATTT
AgeI 289 ACCGGT A^CCGGT
1381 ACCGGT
Alw44I 591 GTGCAC G^TGCAC
737 GTGCAC
ApaLI 591 GTGCAC G^TGCAC
737 GTGCAC
ApoI 1686 GAATTC [GA]^AATT[CT]
2474 AAATTT
BcgI 297 CGAGCATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
564 GCATTTGCCTCG
BsaI 744 GAGACC GGTCTC(1/5)
2671 GAGACC
DraIII 821 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1063 CACAGTGTG
EagI 895 CGGCCG C^GGCCG
899 CGGCCG
EclXI 895 CGGCCG C^GGCCG
899 CGGCCG
Eco52I 895 CGGCCG C^GGCCG
899 CGGCCG
HaeII 85 AGCGCC [GA]GCGC^[CT]
129 AGCGCT
HincII 1625 GTCAAC GT[CT]^[GA]AC
2914 GTTAAC
HindII 1625 GTCAAC GT[CT]^[GA]AC
2914 GTTAAC
MspA1I 892 CAGCGG C[AC]G^C[GT]G
1819 CAGCTG
PinAI 289 ACCGGT A^CCGGT
1381 ACCGGT
XcmI 1751 CCACGCTGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2220 CCATCTTTCATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
Acc65I AccIII AciI AfaI AhdI
AluI AlwNI AocI ApaI Asp718I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MroI MscI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmaCI XmaI