################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AclI AflII AscI AseI AsnI Asp700I AviII BamHI BbeI BbrPI BciVI BclI BfrI BglII BsaAI BsiWI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI EcoO65I EcoRV EcoT22I FbaI FspI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI SacII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 193 GACGTC GACGT^C AccI 1792 GTCTAC GT^[AC][GT]AC AccII 671 CGCG CG^CG AcyI 193 GACGTC G[GA]^CG[CT]C AflIII 924 ACATGT A^C[GA][CT]GT AlwI 355 GATCC GGATC(4/5) Aor51HI 129 AGCGCT AGC^GCT AspI 695 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2110 CCTAGG C^CTAGG BbsI 2361 GAAGAC GAAGAC(2/6) BbvCI 1469 CCTCAGC CCTCAGC(-5/-2) BlnI 2110 CCTAGG C^CTAGG BlpI 889 GCTCAGC GC^T[ACGT]AGC Bpu1102I 889 GCTCAGC GC^T[ACGT]AGC BpuAI 2361 GAAGAC GAAGAC(2/6) BsaBI 790 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 193 GACGTC G[GA]^CG[CT]C BsmBI 191 GAGACG CGTCTC(1/5) BsmI 2521 GAATGC GAATGC(1/-1) BspHI 1718 TCATGA T^CATGA BspLU11I 924 ACATGT A^CATGT BsrBI 41 GAGCGG CCGCTC(-3/-3) BstAPI 1300 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 671 CGCG CG^CG BstXI 478 CCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 354 AGATCC [GA]^GATC[CT] CelII 889 GCTCAGC GC^T[ACGT]AGC CpoI 1383 CGGTCCG CG^G[AT]CCG DraI 3173 TTTAAA TTT^AAA DrdI 1843 GACCCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 484 CCATGG C^C[GA][CT]GG Eco47III 129 AGCGCT AGC^GCT EcoRI 1686 GAATTC G^AATTC FseI 896 GGCCGGCC GGCCGG^CC HgaI 1403 GACGC GACGC(5/10) Hin1I 193 GACGTC G[GA]^CG[CT]C HindIII 1947 AAGCTT A^AGCTT HpaI 2914 GTTAAC GTT^AAC MamI 790 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 354 AGATCC [GA]^GATC[CT] MslI 479 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 671 CGCG CG^CG NaeI 897 GCCGGC GCC^GGC NcoI 484 CCATGG C^CATGG NgoMIV 897 GCCGGC G^CCGGC PflFI 695 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1807 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 676 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1819 CAGCTG CAG^CTG RcaI 1718 TCATGA T^CATGA RsrII 1383 CGGTCCG CG^G[AT]CCG SacI 998 GAGCTC GAGCT^C ScaI 435 AGTACT AGT^ACT SfiI 2040 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 1374 GCATGC GCATG^C TaqI 519 TCGA T^CGA Tth111I 695 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 519 TCGA T^CGA Van91I 1807 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1690 TCTAGA T^CTAGA XhoII 354 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 1686 GAATTC [GA]^AATT[CT] 2474 AAATTT AgeI 289 ACCGGT A^CCGGT 1381 ACCGGT Alw44I 591 GTGCAC G^TGCAC 737 GTGCAC ApaLI 591 GTGCAC G^TGCAC 737 GTGCAC ApoI 1686 GAATTC [GA]^AATT[CT] 2474 AAATTT BcgI 297 CGAGCATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 564 GCATTTGCCTCG BsaI 744 GAGACC GGTCTC(1/5) 2671 GAGACC DraIII 821 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG 1063 CACAGTGTG EagI 895 CGGCCG C^GGCCG 899 CGGCCG EclXI 895 CGGCCG C^GGCCG 899 CGGCCG Eco52I 895 CGGCCG C^GGCCG 899 CGGCCG HaeII 85 AGCGCC [GA]GCGC^[CT] 129 AGCGCT HincII 1625 GTCAAC GT[CT]^[GA]AC 2914 GTTAAC HindII 1625 GTCAAC GT[CT]^[GA]AC 2914 GTTAAC MspA1I 892 CAGCGG C[AC]G^C[GT]G 1819 CAGCTG PinAI 289 ACCGGT A^CCGGT 1381 ACCGGT XcmI 1751 CCACGCTGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2220 CCATCTTTCATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### Acc65I AccIII AciI AfaI AhdI AluI AlwNI AocI ApaI Asp718I AspEI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MroI MscI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmaCI XmaI