##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AspI AviII AvrII
BbeI BcgI BciVI BclI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KspI MamI
MluI MroI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1108 GGTACC G^GTACC
AccII 3 CGCG CG^CG
AseI 2043 ATTAAT AT^TAAT
AsnI 2043 ATTAAT AT^TAAT
Asp700I 146 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1108 GGTACC G^GTACC
BbvCI 489 GCTGAGG CCTCAGC(-5/-2)
BsaI 319 GGTCTC GGTCTC(1/5)
BsmBI 70 GAGACG CGTCTC(1/5)
BspMI 1546 ACCTGC ACCTGC(4/8)
BsrBI 3620 CCGCTC CCGCTC(-3/-3)
BsrFI 103 GCCGGC [GA]^CCGG[CT]
BstUI 3 CGCG CG^CG
Cfr10I 103 GCCGGC [GA]^CCGG[CT]
HaeII 2679 AGCGCC [GA]GCGC^[CT]
HgaI 72 GACGC GACGC(5/10)
KpnI 1108 GGTACC GGTAC^C
MvnI 3 CGCG CG^CG
NaeI 103 GCCGGC GCC^GGC
NgoMIV 103 GCCGGC G^CCGGC
PpuMI 3672 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2043 ATTAAT AT^TAAT
SapI 3335 GCTCTTC GCTCTTC(1/4)
SmaI 91 CCCGGG CCC^GGG
SspI 3908 AATATT AAT^ATT
StuI 1858 AGGCCT AGG^CCT
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
XmnI 146 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1275 GTCTAC GT^[AC][GT]AC
3193 GTAGAC
AspHI 1926 GAGCAC G[AT]GC[AT]^C
2428 GTGCTC
AvaI 91 CCCGGG C^[CT]CG[GA]G
831 CCCGAG
BanI 1108 GGTACC G^G[CT][GA]CC
1686 GGTGCC
BbrPI 620 CACGTG CAC^GTG
3805 CACGTG
BglI 1023 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1326 GCCTGTTGGGC
BsaAI 620 CACGTG [CT]AC^GT[GA]
3805 CACGTG
BsiHKAI 1926 GAGCAC G[AT]GC[AT]^C
2428 GTGCTC
BsmI 849 GCATTC GAATGC(1/-1)
1564 GCATTC
BsoBI 91 CCCGGG C^[CT]CG[GA]G
831 CCCGAG
Bsp1407I 565 TGTACA T^GTACA
3507 TGTACA
BspHI 1651 TCATGA T^CATGA
3696 TCATGA
BspLU11I 2531 ACATGT A^CATGT
2695 ACATGT
BsrGI 565 TGTACA T^GTACA
3507 TGTACA
BstAPI 3647 GCATGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3749 GCATGACATGC
BstXI 1675 CCACAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2358 CCAGACTACTGG
CfoI 2 GCGC GCG^C
2680 GCGC
DraI 327 TTTAAA TTT^AAA
2389 TTTAAA
DsaI 523 CCATGG C^C[GA][CT]GG
2451 CCATGG
EcoNI 1861 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3171 CCTCACCCAGG
EcoRI 615 GAATTC G^AATTC
3496 GAATTC
EcoRV 645 GATATC GAT^ATC
1626 GATATC
HhaI 2 GCGC GCG^C
2680 GCGC
HinP1I 2 GCGC G^CGC
2680 GCGC
HindIII 345 AAGCTT A^AGCTT
775 AAGCTT
MfeI 1458 CAATTG C^AATTG
4024 CAATTG
MunI 1458 CAATTG C^AATTG
4024 CAATTG
NcoI 523 CCATGG C^CATGG
2451 CCATGG
PmaCI 620 CACGTG CAC^GTG
3805 CACGTG
PmlI 620 CACGTG CAC^GTG
3805 CACGTG
PvuII 487 CAGCTG CAG^CTG
3394 CAGCTG
RcaI 1651 TCATGA T^CATGA
3696 TCATGA
SspBI 565 TGTACA T^GTACA
3507 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI AocI ApoI
AvaII BalI BamHI BanII BbsI
BbvI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII