################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AviII AvrII BbeI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI KasI KspI MamI MluI MroI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 1108 GGTACC G^GTACC AccII 3 CGCG CG^CG AseI 2043 ATTAAT AT^TAAT AsnI 2043 ATTAAT AT^TAAT Asp700I 146 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1108 GGTACC G^GTACC BbvCI 489 GCTGAGG CCTCAGC(-5/-2) BsaI 319 GGTCTC GGTCTC(1/5) BsmBI 70 GAGACG CGTCTC(1/5) BspMI 1546 ACCTGC ACCTGC(4/8) BsrBI 3620 CCGCTC CCGCTC(-3/-3) BsrFI 103 GCCGGC [GA]^CCGG[CT] BstUI 3 CGCG CG^CG Cfr10I 103 GCCGGC [GA]^CCGG[CT] HaeII 2679 AGCGCC [GA]GCGC^[CT] HgaI 72 GACGC GACGC(5/10) KpnI 1108 GGTACC GGTAC^C MvnI 3 CGCG CG^CG NaeI 103 GCCGGC GCC^GGC NgoMIV 103 GCCGGC G^CCGGC PpuMI 3672 GGGACCC [GA]G^G[AT]CC[CT] PshBI 2043 ATTAAT AT^TAAT SapI 3335 GCTCTTC GCTCTTC(1/4) SmaI 91 CCCGGG CCC^GGG SspI 3908 AATATT AAT^ATT StuI 1858 AGGCCT AGG^CCT XmaCI 91 CCCGGG C^CCGGG XmaI 91 CCCGGG C^CCGGG XmnI 146 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 1275 GTCTAC GT^[AC][GT]AC 3193 GTAGAC AspHI 1926 GAGCAC G[AT]GC[AT]^C 2428 GTGCTC AvaI 91 CCCGGG C^[CT]CG[GA]G 831 CCCGAG BanI 1108 GGTACC G^G[CT][GA]CC 1686 GGTGCC BbrPI 620 CACGTG CAC^GTG 3805 CACGTG BglI 1023 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1326 GCCTGTTGGGC BsaAI 620 CACGTG [CT]AC^GT[GA] 3805 CACGTG BsiHKAI 1926 GAGCAC G[AT]GC[AT]^C 2428 GTGCTC BsmI 849 GCATTC GAATGC(1/-1) 1564 GCATTC BsoBI 91 CCCGGG C^[CT]CG[GA]G 831 CCCGAG Bsp1407I 565 TGTACA T^GTACA 3507 TGTACA BspHI 1651 TCATGA T^CATGA 3696 TCATGA BspLU11I 2531 ACATGT A^CATGT 2695 ACATGT BsrGI 565 TGTACA T^GTACA 3507 TGTACA BstAPI 3647 GCATGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3749 GCATGACATGC BstXI 1675 CCACAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2358 CCAGACTACTGG CfoI 2 GCGC GCG^C 2680 GCGC DraI 327 TTTAAA TTT^AAA 2389 TTTAAA DsaI 523 CCATGG C^C[GA][CT]GG 2451 CCATGG EcoNI 1861 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3171 CCTCACCCAGG EcoRI 615 GAATTC G^AATTC 3496 GAATTC EcoRV 645 GATATC GAT^ATC 1626 GATATC HhaI 2 GCGC GCG^C 2680 GCGC HinP1I 2 GCGC G^CGC 2680 GCGC HindIII 345 AAGCTT A^AGCTT 775 AAGCTT MfeI 1458 CAATTG C^AATTG 4024 CAATTG MunI 1458 CAATTG C^AATTG 4024 CAATTG NcoI 523 CCATGG C^CATGG 2451 CCATGG PmaCI 620 CACGTG CAC^GTG 3805 CACGTG PmlI 620 CACGTG CAC^GTG 3805 CACGTG PvuII 487 CAGCTG CAG^CTG 3394 CAGCTG RcaI 1651 TCATGA T^CATGA 3696 TCATGA SspBI 565 TGTACA T^GTACA 3507 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI AocI ApoI AvaII BalI BamHI BanII BbsI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII