################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AccIII AclI AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AspI BamHI BbeI BcgI BclI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 3 GACGTC GACGT^C Acc65I 2420 GGTACC G^GTACC AflII 1808 CTTAAG C^TTAAG AhdI 2427 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2062 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2420 GGTACC G^GTACC AspEI 2427 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1705 TGCGCA TGC^GCA BbrPI 1342 CACGTG CAC^GTG BbvCI 782 CCTCAGC CCTCAGC(-5/-2) BciVI 435 GGATAC GTATCC(6/5) BfrI 1808 CTTAAG C^TTAAG BsaAI 1342 CACGTG [CT]AC^GT[GA] BsaI 3872 GGTCTC GGTCTC(1/5) BsaWI 2417 TCCGGT [AT]^CCGG[AT] BsiEI 788 CGACCG CG[GA][CT]^CG BsmBI 3633 CGTCTC CGTCTC(1/5) BsrDI 42 CATTGC GCAATG(2/0) BstAPI 3585 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1743 GGTGACC G^GT[ACGT]ACC BstPI 1743 GGTGACC G^GT[ACGT]ACC DraI 2000 TTTAAA TTT^AAA DraIII 3809 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1781 GACCCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2427 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1743 GGTGACC G^GT[ACGT]ACC EcoRI 2062 GAATTC G^AATTC FspI 1705 TGCGCA TGC^GCA KpnI 2420 GGTACC GGTAC^C NaeI 1254 GCCGGC GCC^GGC NcoI 1739 CCATGG C^CATGG NgoMIV 1254 GCCGGC G^CCGGC PmaCI 1342 CACGTG CAC^GTG PmlI 1342 CACGTG CAC^GTG PshAI 853 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1005 GAGCTC GAGCT^C SapI 2306 GCTCTTC GCTCTTC(1/4) SbfI 2560 CCTGCAGG CCTGCA^GG ScaI 2037 AGTACT AGT^ACT SexAI 1542 ACCAGGT A^CC[AT]GGT Sse8387I 2560 CCTGCAGG CCTGCA^GG TaqI 23 TCGA T^CGA TthHB8I 23 TCGA T^CGA XmnI 2062 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 2696 GTCTAC GT^[AC][GT]AC 3232 GTCTAC AocI 563 CCTCAGG CC^T[ACGT]AGG 830 CCTCAGG BalI 797 TGGCCA TGG^CCA 2151 TGGCCA BglII 1069 AGATCT A^GATCT 2840 AGATCT BlpI 1486 GCTCAGC GC^T[ACGT]AGC 2407 GCTGAGC Bpu1102I 1486 GCTCAGC GC^T[ACGT]AGC 2407 GCTGAGC BssHII 955 GCGCGC G^CGCGC 1137 GCGCGC Bsu36I 563 CCTCAGG CC^T[ACGT]AGG 830 CCTCAGG CelII 1486 GCTCAGC GC^T[ACGT]AGC 2407 GCTGAGC Eco81I 563 CCTCAGG CC^T[ACGT]AGG 830 CCTCAGG HaeII 596 AGCGCC [GA]GCGC^[CT] 3033 AGCGCC MluNI 797 TGGCCA TGG^CCA 2151 TGGCCA MscI 797 TGGCCA TGG^CCA 2151 TGGCCA NspI 1351 ACATGC [GA]CATG^[CT] 3747 ACATGC PflMI 1543 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3038 CCAGACCCTGG TfiI 711 GATTC G^A[AT]TC 1873 GATTC Van91I 1543 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3038 CCAGACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII AvrII BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI