##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccIII AclI AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspI BamHI BbeI BcgI BclI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3 GACGTC GACGT^C
Acc65I 2420 GGTACC G^GTACC
AflII 1808 CTTAAG C^TTAAG
AhdI 2427 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2062 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2420 GGTACC G^GTACC
AspEI 2427 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1705 TGCGCA TGC^GCA
BbrPI 1342 CACGTG CAC^GTG
BbvCI 782 CCTCAGC CCTCAGC(-5/-2)
BciVI 435 GGATAC GTATCC(6/5)
BfrI 1808 CTTAAG C^TTAAG
BsaAI 1342 CACGTG [CT]AC^GT[GA]
BsaI 3872 GGTCTC GGTCTC(1/5)
BsaWI 2417 TCCGGT [AT]^CCGG[AT]
BsiEI 788 CGACCG CG[GA][CT]^CG
BsmBI 3633 CGTCTC CGTCTC(1/5)
BsrDI 42 CATTGC GCAATG(2/0)
BstAPI 3585 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1743 GGTGACC G^GT[ACGT]ACC
BstPI 1743 GGTGACC G^GT[ACGT]ACC
DraI 2000 TTTAAA TTT^AAA
DraIII 3809 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1781 GACCCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2427 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1743 GGTGACC G^GT[ACGT]ACC
EcoRI 2062 GAATTC G^AATTC
FspI 1705 TGCGCA TGC^GCA
KpnI 2420 GGTACC GGTAC^C
NaeI 1254 GCCGGC GCC^GGC
NcoI 1739 CCATGG C^CATGG
NgoMIV 1254 GCCGGC G^CCGGC
PmaCI 1342 CACGTG CAC^GTG
PmlI 1342 CACGTG CAC^GTG
PshAI 853 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1005 GAGCTC GAGCT^C
SapI 2306 GCTCTTC GCTCTTC(1/4)
SbfI 2560 CCTGCAGG CCTGCA^GG
ScaI 2037 AGTACT AGT^ACT
SexAI 1542 ACCAGGT A^CC[AT]GGT
Sse8387I 2560 CCTGCAGG CCTGCA^GG
TaqI 23 TCGA T^CGA
TthHB8I 23 TCGA T^CGA
XmnI 2062 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 2696 GTCTAC GT^[AC][GT]AC
3232 GTCTAC
AocI 563 CCTCAGG CC^T[ACGT]AGG
830 CCTCAGG
BalI 797 TGGCCA TGG^CCA
2151 TGGCCA
BglII 1069 AGATCT A^GATCT
2840 AGATCT
BlpI 1486 GCTCAGC GC^T[ACGT]AGC
2407 GCTGAGC
Bpu1102I 1486 GCTCAGC GC^T[ACGT]AGC
2407 GCTGAGC
BssHII 955 GCGCGC G^CGCGC
1137 GCGCGC
Bsu36I 563 CCTCAGG CC^T[ACGT]AGG
830 CCTCAGG
CelII 1486 GCTCAGC GC^T[ACGT]AGC
2407 GCTGAGC
Eco81I 563 CCTCAGG CC^T[ACGT]AGG
830 CCTCAGG
HaeII 596 AGCGCC [GA]GCGC^[CT]
3033 AGCGCC
MluNI 797 TGGCCA TGG^CCA
2151 TGGCCA
MscI 797 TGGCCA TGG^CCA
2151 TGGCCA
NspI 1351 ACATGC [GA]CATG^[CT]
3747 ACATGC
PflMI 1543 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3038 CCAGACCCTGG
TfiI 711 GATTC G^A[AT]TC
1873 GATTC
Van91I 1543 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3038 CCAGACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AvrII BanI
BanII BbsI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmaCI XmaI