##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
AlwNI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BlnI
BsaAI BsaI BsiEI BsiWI BsmBI
BspDI BspHI BsrBI BsrFI BssHII
BstEII BstPI BstUI BstXI Cfr10I
ClaI CpoI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MluI MluNI MscI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 54 GACGTC GACGT^C
AccI 2609 GTATAC GT^[AC][GT]AC
AccIII 1578 TCCGGA T^CCGGA
AflII 2836 CTTAAG C^TTAAG
AflIII 2444 ACATGT A^C[GA][CT]GT
Alw44I 1384 GTGCAC G^TGCAC
AocI 1670 CCTCAGG CC^T[ACGT]AGG
ApaLI 1384 GTGCAC G^TGCAC
AseI 2876 ATTAAT AT^TAAT
AsnI 2876 ATTAAT AT^TAAT
AspI 2475 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2536 GGATCC G^GATCC
BanII 1539 GGGCTC G[GA]GC[CT]^C
BclI 1691 TGATCA T^GATCA
BcnI 2691 CCGGG CC^[GC]GG
BfrI 2836 CTTAAG C^TTAAG
BglI 1973 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1541 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1541 GCTCAGC GC^T[ACGT]AGC
BsaBI 1510 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1578 TCCGGA T^CCGGA
Bsp1407I 3063 TGTACA T^GTACA
BspEI 1578 TCCGGA T^CCGGA
BspLU11I 2444 ACATGT A^CATGT
BspMI 1440 GCAGGT ACCTGC(4/8)
BsrGI 3063 TGTACA T^GTACA
BssSI 2112 CACGAG CACGAG(-5/-1)
Bst1107I 2609 GTATAC GTA^TAC
BstAPI 3371 GCATATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2264 TTCGAA TT^CGAA
BstZ17I 2609 GTATAC GTA^TAC
Bsu36I 1670 CCTCAGG CC^T[ACGT]AGG
CelII 1541 GCTCAGC GC^T[ACGT]AGC
CfoI 282 GCGC GCG^C
DrdI 1755 GACTTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 162 CCATGG C^C[GA][CT]GG
Eco81I 1670 CCTCAGG CC^T[ACGT]AGG
EcoNI 99 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3054 GAATTC G^AATTC
EcoRV 181 GATATC GAT^ATC
EcoT22I 2748 ATGCAT ATGCA^T
FauI 1546 GCGGG CCCGC(4/6)
FbaI 1691 TGATCA T^GATCA
HhaI 282 GCGC GCG^C
HinP1I 282 GCGC G^CGC
HindIII 885 AAGCTT A^AGCTT
MamI 1510 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1578 TCCGGA T^CCGGA
NciI 2691 CCGGG CC^[GC]GG
NcoI 162 CCATGG C^CATGG
NdeI 2989 CATATG CA^TATG
NsiI 2748 ATGCAT ATGCA^T
NspV 2264 TTCGAA TT^CGAA
PflFI 2475 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2876 ATTAAT AT^TAAT
ScaI 2999 AGTACT AGT^ACT
SfuI 2264 TTCGAA TT^CGAA
SphI 1101 GCATGC GCATG^C
SspBI 3063 TGTACA T^GTACA
Tth111I 2475 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 372 TCTAGA T^CTAGA
XcmI 2703 CCAGAAGAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaI 446 CCCGAG C^[CT]CG[GA]G
2402 CCCGAG
BanI 1981 GGCACC G^G[CT][GA]CC
3211 GGTGCC
BbsI 2831 GAAGAC GAAGAC(2/6)
2365 GTCTTC
BbvCI 592 CCTCAGC CCTCAGC(-5/-2)
1105 GCTGAGG
BglII 612 AGATCT A^GATCT
2467 AGATCT
BpuAI 2831 GAAGAC GAAGAC(2/6)
2365 GTCTTC
BsaWI 399 ACCGGA [AT]^CCGG[AT]
1578 TCCGGA
BseRI 755 CTCCTC GAGGAG(10/8)
2573 CTCCTC
BsgI 3043 CTGCAC GTGCAG(16/14)
1798 GTGCAG
BsoBI 446 CCCGAG C^[CT]CG[GA]G
2402 CCCGAG
MaeII 55 ACGT A^CGT
2149 ACGT
NspI 1101 GCATGC [GA]CATG^[CT]
2444 ACATGT
PshAI 648 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3324 GACCACTGTC
SapI 765 GAAGAGC GCTCTTC(1/4)
3399 GCTCTTC
SmlI 1942 CTCAAG C^T[CT][GA]AG
2836 CTTAAG
StuI 96 AGGCCT AGG^CCT
912 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BbvI
BfaI BmyI BpmI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII