################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AccII AclI AgeI AhdI AlwNI Aor51HI ApaI AscI Asp700I Asp718I AspEI AviII AvrII BalI BbeI BbrPI BcgI BciVI BlnI BsaAI BsaI BsiEI BsiWI BsmBI BspDI BspHI BsrBI BsrFI BssHII BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MluI MluNI MscI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 54 GACGTC GACGT^C AccI 2609 GTATAC GT^[AC][GT]AC AccIII 1578 TCCGGA T^CCGGA AflII 2836 CTTAAG C^TTAAG AflIII 2444 ACATGT A^C[GA][CT]GT Alw44I 1384 GTGCAC G^TGCAC AocI 1670 CCTCAGG CC^T[ACGT]AGG ApaLI 1384 GTGCAC G^TGCAC AseI 2876 ATTAAT AT^TAAT AsnI 2876 ATTAAT AT^TAAT AspI 2475 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2536 GGATCC G^GATCC BanII 1539 GGGCTC G[GA]GC[CT]^C BclI 1691 TGATCA T^GATCA BcnI 2691 CCGGG CC^[GC]GG BfrI 2836 CTTAAG C^TTAAG BglI 1973 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1541 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1541 GCTCAGC GC^T[ACGT]AGC BsaBI 1510 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1578 TCCGGA T^CCGGA Bsp1407I 3063 TGTACA T^GTACA BspEI 1578 TCCGGA T^CCGGA BspLU11I 2444 ACATGT A^CATGT BspMI 1440 GCAGGT ACCTGC(4/8) BsrGI 3063 TGTACA T^GTACA BssSI 2112 CACGAG CACGAG(-5/-1) Bst1107I 2609 GTATAC GTA^TAC BstAPI 3371 GCATATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2264 TTCGAA TT^CGAA BstZ17I 2609 GTATAC GTA^TAC Bsu36I 1670 CCTCAGG CC^T[ACGT]AGG CelII 1541 GCTCAGC GC^T[ACGT]AGC CfoI 282 GCGC GCG^C DrdI 1755 GACTTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 162 CCATGG C^C[GA][CT]GG Eco81I 1670 CCTCAGG CC^T[ACGT]AGG EcoNI 99 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3054 GAATTC G^AATTC EcoRV 181 GATATC GAT^ATC EcoT22I 2748 ATGCAT ATGCA^T FauI 1546 GCGGG CCCGC(4/6) FbaI 1691 TGATCA T^GATCA HhaI 282 GCGC GCG^C HinP1I 282 GCGC G^CGC HindIII 885 AAGCTT A^AGCTT MamI 1510 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1578 TCCGGA T^CCGGA NciI 2691 CCGGG CC^[GC]GG NcoI 162 CCATGG C^CATGG NdeI 2989 CATATG CA^TATG NsiI 2748 ATGCAT ATGCA^T NspV 2264 TTCGAA TT^CGAA PflFI 2475 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2876 ATTAAT AT^TAAT ScaI 2999 AGTACT AGT^ACT SfuI 2264 TTCGAA TT^CGAA SphI 1101 GCATGC GCATG^C SspBI 3063 TGTACA T^GTACA Tth111I 2475 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 372 TCTAGA T^CTAGA XcmI 2703 CCAGAAGAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaI 446 CCCGAG C^[CT]CG[GA]G 2402 CCCGAG BanI 1981 GGCACC G^G[CT][GA]CC 3211 GGTGCC BbsI 2831 GAAGAC GAAGAC(2/6) 2365 GTCTTC BbvCI 592 CCTCAGC CCTCAGC(-5/-2) 1105 GCTGAGG BglII 612 AGATCT A^GATCT 2467 AGATCT BpuAI 2831 GAAGAC GAAGAC(2/6) 2365 GTCTTC BsaWI 399 ACCGGA [AT]^CCGG[AT] 1578 TCCGGA BseRI 755 CTCCTC GAGGAG(10/8) 2573 CTCCTC BsgI 3043 CTGCAC GTGCAG(16/14) 1798 GTGCAG BsoBI 446 CCCGAG C^[CT]CG[GA]G 2402 CCCGAG MaeII 55 ACGT A^CGT 2149 ACGT NspI 1101 GCATGC [GA]CATG^[CT] 2444 ACATGT PshAI 648 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3324 GACCACTGTC SapI 765 GAAGAGC GCTCTTC(1/4) 3399 GCTCTTC SmlI 1942 CTCAAG C^T[CT][GA]AG 2836 CTTAAG StuI 96 AGGCCT AGG^CCT 912 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaII BbvI BfaI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII