##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       BbeI        BbrPI       BcgI        BclI        
BcnI        BglI        BglII       BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
BstAPI      BstBI       BstEII      BstPI       CelII       
Cfr10I      ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HapII       Hin1I       HpaII       KasI        KpnI        
KspI        MaeII       MamI        MluI        MroI        
MspI        NaeI        NarI        NciI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI         2556  GTATAC               GT^[AC][GT]AC
AflII         884  CTTAAG               C^TTAAG
AvrII        1557  CCTAGG               C^CTAGG
BalI         2133  TGGCCA               TGG^CCA
BamHI         124  GGATCC               G^GATCC
BanII         737  GAGCTC               G[GA]GC[CT]^C
BciVI         982  GGATAC               GTATCC(6/5)
BfrI          884  CTTAAG               C^TTAAG
BlnI         1557  CCTAGG               C^CTAGG
BsmI         2825  GCATTC               GAATGC(1/-1)
BspMI         350  ACCTGC               ACCTGC(4/8)
BsrDI         540  GCAATG               GCAATG(2/0)
BssSI         344  CACGAG               CACGAG(-5/-1)
Bst1107I     2556  GTATAC               GTA^TAC
BstZ17I      2556  GTATAC               GTA^TAC
DraI         2279  TTTAAA               TTT^AAA
DraIII        146  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         351  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2782  GAATTC               G^AATTC
EcoRV        2966  GATATC               GAT^ATC
HgaI           84  GACGC                GACGC(5/10)
HpaI         3224  GTTAAC               GTT^AAC
MfeI         2715  CAATTG               C^AATTG
MluNI        2133  TGGCCA               TGG^CCA
MscI         2133  TGGCCA               TGG^CCA
MunI         2715  CAATTG               C^AATTG
NdeI         1889  CATATG               CA^TATG
PvuII         897  CAGCTG               CAG^CTG
SacI          737  GAGCTC               GAGCT^C
ScaI          482  AGTACT               AGT^ACT
StuI         2474  AGGCCT               AGG^CCT
TaqI         1648  TCGA                 T^CGA
TthHB8I      1648  TCGA                 T^CGA
XcmI          171  CCAAGGATTAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII          39  CGCG                 CG^CG
               41  CGCG                
AflIII        218  ACATGT               A^C[GA][CT]GT
             3108  ACATGT              
Alw44I       1562  GTGCAC               G^TGCAC
             2366  GTGCAC              
AlwNI        1629  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1836  CAGAGACTG           
AocI          355  CCTCAGG              CC^T[ACGT]AGG
             2406  CCTGAGG             
ApaLI        1562  GTGCAC               G^TGCAC
             2366  GTGCAC              
BanI           49  GGCACC               G^G[CT][GA]CC
             1925  GGTGCC              
BbsI          166  GAAGAC               GAAGAC(2/6)
              421  GAAGAC              
BbvCI        1225  CCTCAGC              CCTCAGC(-5/-2)
             1449  GCTGAGG             
BpmI         3141  CTCCAG               CTGGAG(16/14)
              876  CTGGAG              
BpuAI         166  GAAGAC               GAAGAC(2/6)
              421  GAAGAC              
BsaI          347  GAGACC               GGTCTC(1/5)
             2852  GGTCTC              
BseRI        2255  GAGGAG               GAGGAG(10/8)
             2258  GAGGAG              
BspLU11I      218  ACATGT               A^CATGT
             3108  ACATGT              
BstUI          39  CGCG                 CG^CG
               41  CGCG                
BstYI         124  GGATCC               [GA]^GATC[CT]
             2339  AGATCC              
Bsu36I        355  CCTCAGG              CC^T[ACGT]AGG
             2406  CCTGAGG             
CfoI            1  GCGC                 GCG^C
               40  GCGC                
DsaI         1929  CCATGG               C^C[GA][CT]GG
             3229  CCATGG              
EaeI           43  CGGCCA               [CT]^GGCC[GA]
             2133  TGGCCA              
Eco81I        355  CCTCAGG              CC^T[ACGT]AGG
             2406  CCTGAGG             
FauI         2488  CCCGC                CCCGC(4/6)
              594  GCGGG               
HhaI            1  GCGC                 GCG^C
               40  GCGC                
HinP1I          1  GCGC                 G^CGC
               40  GCGC                
HincII       2588  GTCAAC               GT[CT]^[GA]AC
             3224  GTTAAC              
HindII       2588  GTCAAC               GT[CT]^[GA]AC
             3224  GTTAAC              
MflI          124  GGATCC               [GA]^GATC[CT]
             2339  AGATCC              
MvnI           39  CGCG                 CG^CG
               41  CGCG                
NcoI         1929  CCATGG               C^CATGG
             3229  CCATGG              
SapI          262  GAAGAGC              GCTCTTC(1/4)
              681  GCTCTTC             
XhoII         124  GGATCC               [GA]^GATC[CT]
             2339  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaI        AvaII       
BbvI        BfaI        BmyI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PshAI       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XmnI