################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII BbeI BbrPI BcgI BclI BcnI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FbaI FseI FspI HaeII HapII Hin1I HpaII KasI KpnI KspI MaeII MamI MluI MroI MspI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 2556 GTATAC GT^[AC][GT]AC AflII 884 CTTAAG C^TTAAG AvrII 1557 CCTAGG C^CTAGG BalI 2133 TGGCCA TGG^CCA BamHI 124 GGATCC G^GATCC BanII 737 GAGCTC G[GA]GC[CT]^C BciVI 982 GGATAC GTATCC(6/5) BfrI 884 CTTAAG C^TTAAG BlnI 1557 CCTAGG C^CTAGG BsmI 2825 GCATTC GAATGC(1/-1) BspMI 350 ACCTGC ACCTGC(4/8) BsrDI 540 GCAATG GCAATG(2/0) BssSI 344 CACGAG CACGAG(-5/-1) Bst1107I 2556 GTATAC GTA^TAC BstZ17I 2556 GTATAC GTA^TAC DraI 2279 TTTAAA TTT^AAA DraIII 146 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 351 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2782 GAATTC G^AATTC EcoRV 2966 GATATC GAT^ATC HgaI 84 GACGC GACGC(5/10) HpaI 3224 GTTAAC GTT^AAC MfeI 2715 CAATTG C^AATTG MluNI 2133 TGGCCA TGG^CCA MscI 2133 TGGCCA TGG^CCA MunI 2715 CAATTG C^AATTG NdeI 1889 CATATG CA^TATG PvuII 897 CAGCTG CAG^CTG SacI 737 GAGCTC GAGCT^C ScaI 482 AGTACT AGT^ACT StuI 2474 AGGCCT AGG^CCT TaqI 1648 TCGA T^CGA TthHB8I 1648 TCGA T^CGA XcmI 171 CCAAGGATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 39 CGCG CG^CG 41 CGCG AflIII 218 ACATGT A^C[GA][CT]GT 3108 ACATGT Alw44I 1562 GTGCAC G^TGCAC 2366 GTGCAC AlwNI 1629 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1836 CAGAGACTG AocI 355 CCTCAGG CC^T[ACGT]AGG 2406 CCTGAGG ApaLI 1562 GTGCAC G^TGCAC 2366 GTGCAC BanI 49 GGCACC G^G[CT][GA]CC 1925 GGTGCC BbsI 166 GAAGAC GAAGAC(2/6) 421 GAAGAC BbvCI 1225 CCTCAGC CCTCAGC(-5/-2) 1449 GCTGAGG BpmI 3141 CTCCAG CTGGAG(16/14) 876 CTGGAG BpuAI 166 GAAGAC GAAGAC(2/6) 421 GAAGAC BsaI 347 GAGACC GGTCTC(1/5) 2852 GGTCTC BseRI 2255 GAGGAG GAGGAG(10/8) 2258 GAGGAG BspLU11I 218 ACATGT A^CATGT 3108 ACATGT BstUI 39 CGCG CG^CG 41 CGCG BstYI 124 GGATCC [GA]^GATC[CT] 2339 AGATCC Bsu36I 355 CCTCAGG CC^T[ACGT]AGG 2406 CCTGAGG CfoI 1 GCGC GCG^C 40 GCGC DsaI 1929 CCATGG C^C[GA][CT]GG 3229 CCATGG EaeI 43 CGGCCA [CT]^GGCC[GA] 2133 TGGCCA Eco81I 355 CCTCAGG CC^T[ACGT]AGG 2406 CCTGAGG FauI 2488 CCCGC CCCGC(4/6) 594 GCGGG HhaI 1 GCGC GCG^C 40 GCGC HinP1I 1 GCGC G^CGC 40 GCGC HincII 2588 GTCAAC GT[CT]^[GA]AC 3224 GTTAAC HindII 2588 GTCAAC GT[CT]^[GA]AC 3224 GTTAAC MflI 124 GGATCC [GA]^GATC[CT] 2339 AGATCC MvnI 39 CGCG CG^CG 41 CGCG NcoI 1929 CCATGG C^CATGG 3229 CCATGG SapI 262 GAAGAGC GCTCTTC(1/4) 681 GCTCTTC XhoII 124 GGATCC [GA]^GATC[CT] 2339 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI