##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BbeI BbrPI BcgI BclI
BcnI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstPI CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HapII Hin1I HpaII KasI KpnI
KspI MaeII MamI MluI MroI
MspI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2556 GTATAC GT^[AC][GT]AC
AflII 884 CTTAAG C^TTAAG
AvrII 1557 CCTAGG C^CTAGG
BalI 2133 TGGCCA TGG^CCA
BamHI 124 GGATCC G^GATCC
BanII 737 GAGCTC G[GA]GC[CT]^C
BciVI 982 GGATAC GTATCC(6/5)
BfrI 884 CTTAAG C^TTAAG
BlnI 1557 CCTAGG C^CTAGG
BsmI 2825 GCATTC GAATGC(1/-1)
BspMI 350 ACCTGC ACCTGC(4/8)
BsrDI 540 GCAATG GCAATG(2/0)
BssSI 344 CACGAG CACGAG(-5/-1)
Bst1107I 2556 GTATAC GTA^TAC
BstZ17I 2556 GTATAC GTA^TAC
DraI 2279 TTTAAA TTT^AAA
DraIII 146 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 351 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2782 GAATTC G^AATTC
EcoRV 2966 GATATC GAT^ATC
HgaI 84 GACGC GACGC(5/10)
HpaI 3224 GTTAAC GTT^AAC
MfeI 2715 CAATTG C^AATTG
MluNI 2133 TGGCCA TGG^CCA
MscI 2133 TGGCCA TGG^CCA
MunI 2715 CAATTG C^AATTG
NdeI 1889 CATATG CA^TATG
PvuII 897 CAGCTG CAG^CTG
SacI 737 GAGCTC GAGCT^C
ScaI 482 AGTACT AGT^ACT
StuI 2474 AGGCCT AGG^CCT
TaqI 1648 TCGA T^CGA
TthHB8I 1648 TCGA T^CGA
XcmI 171 CCAAGGATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 39 CGCG CG^CG
41 CGCG
AflIII 218 ACATGT A^C[GA][CT]GT
3108 ACATGT
Alw44I 1562 GTGCAC G^TGCAC
2366 GTGCAC
AlwNI 1629 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1836 CAGAGACTG
AocI 355 CCTCAGG CC^T[ACGT]AGG
2406 CCTGAGG
ApaLI 1562 GTGCAC G^TGCAC
2366 GTGCAC
BanI 49 GGCACC G^G[CT][GA]CC
1925 GGTGCC
BbsI 166 GAAGAC GAAGAC(2/6)
421 GAAGAC
BbvCI 1225 CCTCAGC CCTCAGC(-5/-2)
1449 GCTGAGG
BpmI 3141 CTCCAG CTGGAG(16/14)
876 CTGGAG
BpuAI 166 GAAGAC GAAGAC(2/6)
421 GAAGAC
BsaI 347 GAGACC GGTCTC(1/5)
2852 GGTCTC
BseRI 2255 GAGGAG GAGGAG(10/8)
2258 GAGGAG
BspLU11I 218 ACATGT A^CATGT
3108 ACATGT
BstUI 39 CGCG CG^CG
41 CGCG
BstYI 124 GGATCC [GA]^GATC[CT]
2339 AGATCC
Bsu36I 355 CCTCAGG CC^T[ACGT]AGG
2406 CCTGAGG
CfoI 1 GCGC GCG^C
40 GCGC
DsaI 1929 CCATGG C^C[GA][CT]GG
3229 CCATGG
EaeI 43 CGGCCA [CT]^GGCC[GA]
2133 TGGCCA
Eco81I 355 CCTCAGG CC^T[ACGT]AGG
2406 CCTGAGG
FauI 2488 CCCGC CCCGC(4/6)
594 GCGGG
HhaI 1 GCGC GCG^C
40 GCGC
HinP1I 1 GCGC G^CGC
40 GCGC
HincII 2588 GTCAAC GT[CT]^[GA]AC
3224 GTTAAC
HindII 2588 GTCAAC GT[CT]^[GA]AC
3224 GTTAAC
MflI 124 GGATCC [GA]^GATC[CT]
2339 AGATCC
MvnI 39 CGCG CG^CG
41 CGCG
NcoI 1929 CCATGG C^CATGG
3229 CCATGG
SapI 262 GAAGAGC GCTCTTC(1/4)
681 GCTCTTC
XhoII 124 GGATCC [GA]^GATC[CT]
2339 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BbvI BfaI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshAI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XmnI