##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI BciVI BfrI
BlpI Bpu1102I BsaBI BsiWI BsmI
BspDI BssHII BstAPI BstBI CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoRI EcoRV FseI HpaI
MamI MfeI MluI MseI MunI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tru9I
Tth111I Van91I XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1763 GTATAC GT^[AC][GT]AC
AcsI 2356 AAATTT [GA]^AATT[CT]
AcyI 1796 GGCGCC G[GA]^CG[CT]C
AflIII 2404 ACATGT A^C[GA][CT]GT
AlwNI 2560 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2753 CCTGAGG CC^T[ACGT]AGG
ApoI 2356 AAATTT [GA]^AATT[CT]
Asp700I 2787 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1117 TGCGCA TGC^GCA
AvrII 266 CCTAGG C^CTAGG
BbeI 1796 GGCGCC GGCGC^C
BbrPI 864 CACGTG CAC^GTG
BbvCI 3386 GCTGAGG CCTCAGC(-5/-2)
BclI 2384 TGATCA T^GATCA
BglI 2865 GCCATGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 919 AGATCT A^GATCT
BlnI 266 CCTAGG C^CTAGG
BsaAI 864 CACGTG [CT]AC^GT[GA]
BsaHI 1796 GGCGCC G[GA]^CG[CT]C
BsaI 770 GAGACC GGTCTC(1/5)
Bsp1407I 2074 TGTACA T^GTACA
BspLU11I 2404 ACATGT A^CATGT
BsrBI 3092 CCGCTC CCGCTC(-3/-3)
BsrDI 1031 CATTGC GCAATG(2/0)
BsrFI 1681 GCCGGT [GA]^CCGG[CT]
BsrGI 2074 TGTACA T^GTACA
BssSI 750 CACGAG CACGAG(-5/-1)
Bst1107I 1763 GTATAC GTA^TAC
BstZ17I 1763 GTATAC GTA^TAC
Bsu36I 2753 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1681 GCCGGT [GA]^CCGG[CT]
DraIII 1949 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2753 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2506 ATGCAT ATGCA^T
FbaI 2384 TGATCA T^GATCA
FspI 1117 TGCGCA TGC^GCA
HaeII 1796 GGCGCC [GA]GCGC^[CT]
Hin1I 1796 GGCGCC G[GA]^CG[CT]C
HincII 2292 GTCAAC GT[CT]^[GA]AC
HindII 2292 GTCAAC GT[CT]^[GA]AC
HindIII 2647 AAGCTT A^AGCTT
KasI 1796 GGCGCC G^GCGCC
NarI 1796 GGCGCC GG^CGCC
NdeI 3489 CATATG CA^TATG
NsiI 2506 ATGCAT ATGCA^T
PmaCI 864 CACGTG CAC^GTG
PmlI 864 CACGTG CAC^GTG
PshAI 1263 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1871 AGTACT AGT^ACT
SexAI 712 ACCAGGT A^CC[AT]GGT
SfoI 1796 GGCGCC GGC^GCC
SspBI 2074 TGTACA T^GTACA
XbaI 273 TCTAGA T^CTAGA
XmnI 2787 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 2267 TCCGGA T^CCGGA
3001 TCCGGA
ApaI 391 GGGCCC GGGCC^C
2596 GGGCCC
BalI 1202 TGGCCA TGG^CCA
1604 TGGCCA
BbsI 875 GAAGAC GAAGAC(2/6)
2146 GTCTTC
BpuAI 875 GAAGAC GAAGAC(2/6)
2146 GTCTTC
BsaWI 2267 TCCGGA [AT]^CCGG[AT]
3001 TCCGGA
BseAI 2267 TCCGGA T^CCGGA
3001 TCCGGA
BsiEI 211 CGGCCG CG[GA][CT]^CG
1378 CGGCCG
BsmBI 1655 CGTCTC CGTCTC(1/5)
277 GAGACG
BspEI 2267 TCCGGA T^CCGGA
3001 TCCGGA
EagI 211 CGGCCG C^GGCCG
1378 CGGCCG
EarI 3354 CTCTTC CTCTTC(1/4)
764 GAAGAG
EclXI 211 CGGCCG C^GGCCG
1378 CGGCCG
Eco52I 211 CGGCCG C^GGCCG
1378 CGGCCG
Ksp632I 3354 CTCTTC CTCTTC(1/4)
764 GAAGAG
KspI 173 CCGCGG CCGC^GG
230 CCGCGG
MluNI 1202 TGGCCA TGG^CCA
1604 TGGCCA
MroI 2267 TCCGGA T^CCGGA
3001 TCCGGA
MscI 1202 TGGCCA TGG^CCA
1604 TGGCCA
SacII 173 CCGCGG CCGC^GG
230 CCGCGG
SmaI 103 CCCGGG CCC^GGG
964 CCCGGG
SmlI 830 CTTGAG C^T[CT][GA]AG
2136 CTTGAG
XcmI 3114 CCACTGAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3419 CCAGTCCTTCCATGG
XmaCI 103 CCCGGG C^CCGGG
964 CCCGGG
XmaI 103 CCCGGG C^CCGGG
964 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI Asp718I AspHI AvaI AvaII
BamHI BanI BanII BbvI BcgI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII