##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Aor51HI ApaI AscI AspI AvaI
AviII BbeI BciVI BcnI BglI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI CfoI Cfr10I ClaI
CpoI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoRV FauI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KspI MluI MroI MslI
MspI NaeI NarI NciI NcoI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2224 GGTACC G^GTACC
AccII 2834 CGCG CG^CG
AflII 2602 CTTAAG C^TTAAG
AflIII 529 ACGTGT A^C[GA][CT]GT
AhdI 2893 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2689 GTGCAC G^TGCAC
AlwNI 7 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1666 CCTGAGG CC^T[ACGT]AGG
ApaLI 2689 GTGCAC G^TGCAC
AseI 961 ATTAAT AT^TAAT
AsnI 961 ATTAAT AT^TAAT
Asp718I 2224 GGTACC G^GTACC
AspEI 2893 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2220 CCTAGG C^CTAGG
BamHI 1879 GGATCC G^GATCC
BanI 2224 GGTACC G^G[CT][GA]CC
BbrPI 528 CACGTG CAC^GTG
BbvCI 2943 GCTGAGG CCTCAGC(-5/-2)
BcgI 689 GCAGCCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2602 CTTAAG C^TTAAG
BlnI 2220 CCTAGG C^CTAGG
BsaAI 528 CACGTG [CT]AC^GT[GA]
BsaBI 1647 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 452 CTCCTC GAGGAG(10/8)
BsmI 794 GCATTC GAATGC(1/-1)
BsrDI 2971 CATTGC GCAATG(2/0)
Bst1107I 677 GTATAC GTA^TAC
BstAPI 1342 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1470 GGTGACC G^GT[ACGT]ACC
BstPI 1470 GGTGACC G^GT[ACGT]ACC
BstUI 2834 CGCG CG^CG
BstZ17I 677 GTATAC GTA^TAC
Bsu36I 1666 CCTGAGG CC^T[ACGT]AGG
DraII 326 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1634 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2893 GACATGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1666 CCTGAGG CC^T[ACGT]AGG
EcoO109I 326 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1470 GGTGACC G^GT[ACGT]ACC
EcoT22I 1918 ATGCAT ATGCA^T
HgaI 2832 GACGC GACGC(5/10)
KpnI 2224 GGTACC GGTAC^C
MamI 1647 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2834 CGCG CG^CG
NsiI 1918 ATGCAT ATGCA^T
PmaCI 528 CACGTG CAC^GTG
PmlI 528 CACGTG CAC^GTG
PpuMI 326 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 961 ATTAAT AT^TAAT
PstI 2148 CTGCAG CTGCA^G
SapI 337 GAAGAGC GCTCTTC(1/4)
SfcI 2148 CTGCAG C^T[GA][CT]AG
StuI 144 AGGCCT AGG^CCT
XbaI 2208 TCTAGA T^CTAGA
XcmI 2409 CCAAAAGATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 161 GTCTAC GT^[AC][GT]AC
677 GTATAC
Asp700I 1738 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1819 GAAATGCTTC
BanII 523 GAGCTC G[GA]GC[CT]^C
2515 GAGCTC
BbsI 2890 GAAGAC GAAGAC(2/6)
1872 GTCTTC
BclI 2001 TGATCA T^GATCA
2565 TGATCA
BglII 275 AGATCT A^GATCT
2963 AGATCT
BpuAI 2890 GAAGAC GAAGAC(2/6)
1872 GTCTTC
BstF5I 1663 CATCC GGATG(2/0)
915 GGATG
BstXI 2439 CCATGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2523 CCAGGACACTGG
DrdI 1558 GACTATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2800 GACACTCAAGTC
EcoRI 1077 GAATTC G^AATTC
3189 GAATTC
FbaI 2001 TGATCA T^GATCA
2565 TGATCA
FokI 1663 CATCC GGATG(9/13)
915 GGATG
HindIII 1018 AAGCTT A^AGCTT
1208 AAGCTT
MfeI 152 CAATTG C^AATTG
2283 CAATTG
MunI 152 CAATTG C^AATTG
2283 CAATTG
NdeI 1792 CATATG CA^TATG
2005 CATATG
NheI 1405 GCTAGC G^CTAGC
3020 GCTAGC
PvuII 217 CAGCTG CAG^CTG
1034 CAGCTG
SacI 523 GAGCTC GAGCT^C
2515 GAGCTC
SphI 662 GCATGC GCATG^C
1920 GCATGC
SspI 3212 AATATT AAT^ATT
3225 AATATT
TaqI 438 TCGA T^CGA
1291 TCGA
TthHB8I 438 TCGA T^CGA
1291 TCGA
XmnI 1738 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1819 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII