##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AvrII BamHI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FseI
HincII HindII HpaI KspI MamI
MfeI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacII SalI SapI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 3005 GGTACC G^GTACC
AflIII 1944 ACGCGT A^C[GA][CT]GT
AhdI 517 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3005 GGTACC G^GTACC
AspEI 517 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2477 GGCGCC GGCGC^C
BbrPI 1170 CACGTG CAC^GTG
BcgI 2329 GCATCGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1037 TGATCA T^GATCA
BfaI 2103 CTAG C^TAG
BsaAI 1170 CACGTG [CT]AC^GT[GA]
BsaWI 2057 ACCGGA [AT]^CCGG[AT]
BsiEI 1466 CGATCG CG[GA][CT]^CG
BsmI 120 GAATGC GAATGC(1/-1)
BssSI 531 CACGAG CACGAG(-5/-1)
BstEII 1380 GGTCACC G^GT[ACGT]ACC
BstPI 1380 GGTCACC G^GT[ACGT]ACC
BstXI 3116 CCATCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1043 GACCATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 517 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1380 GGTCACC G^GT[ACGT]ACC
EcoRI 477 GAATTC G^AATTC
FbaI 1037 TGATCA T^GATCA
HaeII 2477 GGCGCC [GA]GCGC^[CT]
HindIII 1788 AAGCTT A^AGCTT
KasI 2477 GGCGCC G^GCGCC
KpnI 3005 GGTACC GGTAC^C
MaeI 2103 CTAG C^TAG
MluI 1944 ACGCGT A^CGCGT
NarI 2477 GGCGCC GG^CGCC
PmaCI 1170 CACGTG CAC^GTG
PmlI 1170 CACGTG CAC^GTG
PvuI 1466 CGATCG CGAT^CG
SacI 2214 GAGCTC GAGCT^C
SbfI 2549 CCTGCAGG CCTGCA^GG
ScaI 228 AGTACT AGT^ACT
SexAI 1384 ACCAGGT A^CC[AT]GGT
SfoI 2477 GGCGCC GGC^GCC
Sse8387I 2549 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1945 CGCG CG^CG
2588 CGCG
AcsI 147 GAATTT [GA]^AATT[CT]
477 GAATTC
ApoI 147 GAATTT [GA]^AATT[CT]
477 GAATTC
Asp700I 1457 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1471 GAACATCTTC
AspI 1375 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1487 GACGATGTC
AviII 748 TGCGCA TGC^GCA
1660 TGCGCA
BsrDI 92 CATTGC GCAATG(2/0)
170 CATTGC
BstAPI 852 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2222 GCAGGATCTGC
BstUI 1945 CGCG CG^CG
2588 CGCG
BstYI 1918 GGATCT [GA]^GATC[CT]
2225 GGATCT
DraIII 2504 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3231 CACACAGTG
EcoNI 746 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2549 CCTGCAGGAGG
FspI 748 TGCGCA TGC^GCA
1660 TGCGCA
MflI 1918 GGATCT [GA]^GATC[CT]
2225 GGATCT
MseI 1048 TTAA T^TAA
3306 TTAA
MvnI 1945 CGCG CG^CG
2588 CGCG
PflFI 1375 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1487 GACGATGTC
SfiI 863 GGCCTCTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
917 GGCCGAGGAGGCC
SmaI 1148 CCCGGG CCC^GGG
2762 CCCGGG
StuI 2005 AGGCCT AGG^CCT
2696 AGGCCT
Tru9I 1048 TTAA T^TAA
3306 TTAA
Tth111I 1375 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1487 GACGATGTC
XhoII 1918 GGATCT [GA]^GATC[CT]
2225 GGATCT
XmaCI 1148 CCCGGG C^CCGGG
2762 CCCGGG
XmaI 1148 CCCGGG C^CCGGG
2762 CCCGGG
XmnI 1457 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1471 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI