################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AvrII BamHI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FseI HincII HindII HpaI KspI MamI MfeI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SapI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 3005 GGTACC G^GTACC AflIII 1944 ACGCGT A^C[GA][CT]GT AhdI 517 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 3005 GGTACC G^GTACC AspEI 517 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2477 GGCGCC GGCGC^C BbrPI 1170 CACGTG CAC^GTG BcgI 2329 GCATCGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1037 TGATCA T^GATCA BfaI 2103 CTAG C^TAG BsaAI 1170 CACGTG [CT]AC^GT[GA] BsaWI 2057 ACCGGA [AT]^CCGG[AT] BsiEI 1466 CGATCG CG[GA][CT]^CG BsmI 120 GAATGC GAATGC(1/-1) BssSI 531 CACGAG CACGAG(-5/-1) BstEII 1380 GGTCACC G^GT[ACGT]ACC BstPI 1380 GGTCACC G^GT[ACGT]ACC BstXI 3116 CCATCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 1043 GACCATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 517 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1380 GGTCACC G^GT[ACGT]ACC EcoRI 477 GAATTC G^AATTC FbaI 1037 TGATCA T^GATCA HaeII 2477 GGCGCC [GA]GCGC^[CT] HindIII 1788 AAGCTT A^AGCTT KasI 2477 GGCGCC G^GCGCC KpnI 3005 GGTACC GGTAC^C MaeI 2103 CTAG C^TAG MluI 1944 ACGCGT A^CGCGT NarI 2477 GGCGCC GG^CGCC PmaCI 1170 CACGTG CAC^GTG PmlI 1170 CACGTG CAC^GTG PvuI 1466 CGATCG CGAT^CG SacI 2214 GAGCTC GAGCT^C SbfI 2549 CCTGCAGG CCTGCA^GG ScaI 228 AGTACT AGT^ACT SexAI 1384 ACCAGGT A^CC[AT]GGT SfoI 2477 GGCGCC GGC^GCC Sse8387I 2549 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 1945 CGCG CG^CG 2588 CGCG AcsI 147 GAATTT [GA]^AATT[CT] 477 GAATTC ApoI 147 GAATTT [GA]^AATT[CT] 477 GAATTC Asp700I 1457 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1471 GAACATCTTC AspI 1375 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1487 GACGATGTC AviII 748 TGCGCA TGC^GCA 1660 TGCGCA BsrDI 92 CATTGC GCAATG(2/0) 170 CATTGC BstAPI 852 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2222 GCAGGATCTGC BstUI 1945 CGCG CG^CG 2588 CGCG BstYI 1918 GGATCT [GA]^GATC[CT] 2225 GGATCT DraIII 2504 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 3231 CACACAGTG EcoNI 746 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2549 CCTGCAGGAGG FspI 748 TGCGCA TGC^GCA 1660 TGCGCA MflI 1918 GGATCT [GA]^GATC[CT] 2225 GGATCT MseI 1048 TTAA T^TAA 3306 TTAA MvnI 1945 CGCG CG^CG 2588 CGCG PflFI 1375 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1487 GACGATGTC SfiI 863 GGCCTCTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 917 GGCCGAGGAGGCC SmaI 1148 CCCGGG CCC^GGG 2762 CCCGGG StuI 2005 AGGCCT AGG^CCT 2696 AGGCCT Tru9I 1048 TTAA T^TAA 3306 TTAA Tth111I 1375 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1487 GACGATGTC XhoII 1918 GGATCT [GA]^GATC[CT] 2225 GGATCT XmaCI 1148 CCCGGG C^CCGGG 2762 CCCGGG XmaI 1148 CCCGGG C^CCGGG 2762 CCCGGG XmnI 1457 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1471 GAACATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI