##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AlwNI AscI AseI AsnI AvrII
BbvCI BfrI BlnI BsaBI BseAI
BsiWI Bsp1407I BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI HgaI HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI SacII SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2872 GGTACC G^GTACC
AccII 1937 CGCG CG^CG
AcsI 2968 AAATTT [GA]^AATT[CT]
AhdI 980 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 26 GTGCAC G^TGCAC
ApaLI 26 GTGCAC G^TGCAC
ApoI 2968 AAATTT [GA]^AATT[CT]
Asp718I 2872 GGTACC G^GTACC
AspEI 980 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 219 TGCGCA TGC^GCA
BbrPI 1691 CACGTG CAC^GTG
BciVI 1549 GTATCC GTATCC(6/5)
BglII 808 AGATCT A^GATCT
BlpI 3100 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3100 GCTCAGC GC^T[ACGT]AGC
BsaWI 1308 ACCGGA [AT]^CCGG[AT]
BsiEI 1315 CGACCG CG[GA][CT]^CG
BsmBI 1858 CGTCTC CGTCTC(1/5)
BsmI 2389 GAATGC GAATGC(1/-1)
BspDI 2174 ATCGAT AT^CGAT
BsrBI 908 GAGCGG CCGCTC(-3/-3)
BsrDI 534 GCAATG GCAATG(2/0)
BstAPI 2263 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2399 GGTGACC G^GT[ACGT]ACC
BstPI 2399 GGTGACC G^GT[ACGT]ACC
BstUI 1937 CGCG CG^CG
CelII 3100 GCTCAGC GC^T[ACGT]AGC
ClaI 2174 ATCGAT AT^CGAT
CpoI 660 CGGACCG CG^G[AT]CCG
Eam1105I 980 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2399 GGTGACC G^GT[ACGT]ACC
FspI 219 TGCGCA TGC^GCA
HincII 758 GTCGAC GT[CT]^[GA]AC
HindII 758 GTCGAC GT[CT]^[GA]AC
KpnI 2872 GGTACC GGTAC^C
MvnI 1937 CGCG CG^CG
PaeR7I 3088 CTCGAG C^TCGAG
PmaCI 1691 CACGTG CAC^GTG
PmlI 1691 CACGTG CAC^GTG
RsrII 660 CGGACCG CG^G[AT]CCG
SacI 320 GAGCTC GAGCT^C
SalI 758 GTCGAC G^TCGAC
SapI 1996 GCTCTTC GCTCTTC(1/4)
ScaI 607 AGTACT AGT^ACT
XhoI 3088 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1357 GGCGCC G[GA]^CG[CT]C
1763 GGCGCC
Aor51HI 1023 AGCGCT AGC^GCT
3013 AGCGCT
Asp700I 289 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1732 GAAGGGCTTC
AspHI 26 GTGCAC G[AT]GC[AT]^C
320 GAGCTC
BamHI 1323 GGATCC G^GATCC
1431 GGATCC
BbeI 1357 GGCGCC GGCGC^C
1763 GGCGCC
BclI 147 TGATCA T^GATCA
176 TGATCA
BsaAI 1208 TACGTG [CT]AC^GT[GA]
1691 CACGTG
BsaHI 1357 GGCGCC G[GA]^CG[CT]C
1763 GGCGCC
BsaI 1124 GAGACC GGTCTC(1/5)
3133 GAGACC
BsiHKAI 26 GTGCAC G[AT]GC[AT]^C
320 GAGCTC
BspHI 519 TCATGA T^CATGA
1173 TCATGA
BspLU11I 396 ACATGT A^CATGT
819 ACATGT
BstXI 726 CCATCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2662 CCAGGGGTTTGG
DraI 2554 TTTAAA TTT^AAA
2709 TTTAAA
DraIII 2264 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2863 CACACAGTG
DrdI 818 GACATGTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1841 GACAGGCGGGTC
Eco47III 1023 AGCGCT AGC^GCT
3013 AGCGCT
FbaI 147 TGATCA T^GATCA
176 TGATCA
Hin1I 1357 GGCGCC G[GA]^CG[CT]C
1763 GGCGCC
KasI 1357 GGCGCC G^GCGCC
1763 GGCGCC
NarI 1357 GGCGCC GG^CGCC
1763 GGCGCC
NcoI 1804 CCATGG C^CATGG
2541 CCATGG
PflMI 477 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3228 CCACATACTGG
PvuII 895 CAGCTG CAG^CTG
3103 CAGCTG
RcaI 519 TCATGA T^CATGA
1173 TCATGA
SbfI 2694 CCTGCAGG CCTGCA^GG
3180 CCTGCAGG
SfoI 1357 GGCGCC GGC^GCC
1763 GGCGCC
SmlI 3088 CTCGAG C^T[CT][GA]AG
3261 CTCAAG
Sse8387I 2694 CCTGCAGG CCTGCA^GG
3180 CCTGCAGG
Tsp509I 2722 AATT ^AATT
2969 AATT
Van91I 477 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3228 CCACATACTGG
XmnI 289 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1732 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AfaI AflIII AluI
AlwI AocI ApaI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI Tth111I
TthHB8I XbaI XhoII