##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AflII AflIII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbvCI
BfrI BglII BlnI BsiWI BspHI
BspLU11I Bst1107I BstBI BstZ17I CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoRV FseI HindIII HpaI
KpnI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI PvuI RcaI RsrII SexAI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2776 GTCGAC GT^[AC][GT]AC
AclI 2141 AACGTT AA^CGTT
AocI 87 CCTCAGG CC^T[ACGT]AGG
AviII 2915 TGCGCA TGC^GCA
BamHI 3165 GGATCC G^GATCC
BbrPI 2293 CACGTG CAC^GTG
BciVI 2590 GGATAC GTATCC(6/5)
BsaAI 2293 CACGTG [CT]AC^GT[GA]
BsaBI 2128 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3382 GAGACC GGTCTC(1/5)
BsiEI 315 CGACCG CG[GA][CT]^CG
Bsp1407I 1400 TGTACA T^GTACA
BspDI 475 ATCGAT AT^CGAT
BsrGI 1400 TGTACA T^GTACA
BssHII 3219 GCGCGC G^CGCGC
BstAPI 3288 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 59 GGTCACC G^GT[ACGT]ACC
BstPI 59 GGTCACC G^GT[ACGT]ACC
Bsu36I 87 CCTCAGG CC^T[ACGT]AGG
ClaI 475 ATCGAT AT^CGAT
Eco81I 87 CCTCAGG CC^T[ACGT]AGG
EcoO65I 59 GGTCACC G^GT[ACGT]ACC
EcoRI 3613 GAATTC G^AATTC
EcoT22I 3426 ATGCAT ATGCA^T
FspI 2915 TGCGCA TGC^GCA
KspI 3592 CCGCGG CCGC^GG
MamI 2128 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3314 CCATGG C^CATGG
NsiI 3426 ATGCAT ATGCA^T
PaeR7I 1000 CTCGAG C^TCGAG
PmaCI 2293 CACGTG CAC^GTG
PmlI 2293 CACGTG CAC^GTG
PshAI 1657 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2141 AACGTT AA^CGTT
SacII 3592 CCGCGG CCGC^GG
SalI 2776 GTCGAC G^TCGAC
SapI 3252 GCTCTTC GCTCTTC(1/4)
SbfI 3987 CCTGCAGG CCTGCA^GG
SphI 99 GCATGC GCATG^C
Sse8387I 3987 CCTGCAGG CCTGCA^GG
SspBI 1400 TGTACA T^GTACA
XhoI 1000 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 273 TCCGGA T^CCGGA
2924 TCCGGA
Alw44I 2511 GTGCAC G^TGCAC
2768 GTGCAC
Aor51HI 764 AGCGCT AGC^GCT
2899 AGCGCT
ApaI 2308 GGGCCC GGGCC^C
3921 GGGCCC
ApaLI 2511 GTGCAC G^TGCAC
2768 GTGCAC
BcgI 467 GCAAGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1974 GCAACCCCATCG
BclI 2627 TGATCA T^GATCA
3512 TGATCA
BseAI 273 TCCGGA T^CCGGA
2924 TCCGGA
BsmBI 358 GAGACG CGTCTC(1/5)
3601 GAGACG
BspEI 273 TCCGGA T^CCGGA
2924 TCCGGA
BsrDI 2375 GCAATG GCAATG(2/0)
3065 GCAATG
BssSI 2755 CACGAG CACGAG(-5/-1)
2771 CACGAG
Eco47III 764 AGCGCT AGC^GCT
2899 AGCGCT
FbaI 2627 TGATCA T^GATCA
3512 TGATCA
MroI 273 TCCGGA T^CCGGA
2924 TCCGGA
MseI 1510 TTAA T^TAA
4095 TTAA
SacI 898 GAGCTC GAGCT^C
3178 GAGCTC
ScaI 1496 AGTACT AGT^ACT
1784 AGTACT
SfiI 688 GGCCATGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2261 GGCCCTTCCGGCC
SmaI 2022 CCCGGG CCC^GGG
3398 CCCGGG
Tru9I 1510 TTAA T^TAA
4095 TTAA
XmaCI 2022 CCCGGG C^CCGGG
3398 CCCGGG
XmaI 2022 CCCGGG C^CCGGG
3398 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII