##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BalI BamHI BbvCI BcgI BfrI
BglII BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstXI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI HgaI HpaI KpnI
KspI MfeI MluNI MscI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 3096 CGCG CG^CG
AccIII 2742 TCCGGA T^CCGGA
AcyI 611 GGCGCC G[GA]^CG[CT]C
AhdI 895 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2869 CCTGAGG CC^T[ACGT]AGG
AspEI 895 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 43 CCCGAG C^[CT]CG[GA]G
AvrII 217 CCTAGG C^CTAGG
BbeI 611 GGCGCC GGCGC^C
BbrPI 2898 CACGTG CAC^GTG
BlnI 217 CCTAGG C^CTAGG
BlpI 697 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 697 GCTCAGC GC^T[ACGT]AGC
BsaAI 2898 CACGTG [CT]AC^GT[GA]
BsaHI 611 GGCGCC G[GA]^CG[CT]C
BsaI 575 GGTCTC GGTCTC(1/5)
BseAI 2742 TCCGGA T^CCGGA
BsoBI 43 CCCGAG C^[CT]CG[GA]G
BspEI 2742 TCCGGA T^CCGGA
BspLU11I 933 ACATGT A^CATGT
BsrFI 2015 GCCGGT [GA]^CCGG[CT]
Bst1107I 2294 GTATAC GTA^TAC
BstUI 3096 CGCG CG^CG
BstZ17I 2294 GTATAC GTA^TAC
Bsu36I 2869 CCTGAGG CC^T[ACGT]AGG
CelII 697 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2015 GCCGGT [GA]^CCGG[CT]
DraII 1431 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 2149 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 452 CCATGG C^C[GA][CT]GG
EaeI 825 CGGCCA [CT]^GGCC[GA]
Eam1105I 895 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2869 CCTGAGG CC^T[ACGT]AGG
EcoNI 2520 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1431 AGGCCCT [GA]G^G[ACGT]CC[CT]
Hin1I 611 GGCGCC G[GA]^CG[CT]C
KasI 611 GGCGCC G^GCGCC
MluI 3095 ACGCGT A^CGCGT
MroI 2742 TCCGGA T^CCGGA
MvnI 3096 CGCG CG^CG
NarI 611 GGCGCC GG^CGCC
NcoI 452 CCATGG C^CATGG
PacI 1225 TTAATTAA TTAAT^TAA
PflMI 841 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2898 CACGTG CAC^GTG
PmlI 2898 CACGTG CAC^GTG
SacI 212 GAGCTC GAGCT^C
SexAI 3280 ACCTGGT A^CC[AT]GGT
SfoI 611 GGCGCC GGC^GCC
SmlI 925 CTTGAG C^T[CT][GA]AG
SpeI 2122 ACTAGT A^CTAGT
SspI 1983 AATATT AAT^ATT
TaqI 1437 TCGA T^CGA
TthHB8I 1437 TCGA T^CGA
Van91I 841 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 933 ACATGT A^C[GA][CT]GT
3095 ACGCGT
AlwNI 347 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1816 CAGCAGCTG
Asp700I 1379 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2048 GAAGTAATTC
AspHI 212 GAGCTC G[AT]GC[AT]^C
1355 GTGCTC
BbsI 1461 GAAGAC GAAGAC(2/6)
2095 GTCTTC
BclI 882 TGATCA T^GATCA
3189 TGATCA
BcnI 1334 CCGGG CC^[GC]GG
1864 CCCGG
BglI 1884 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3424 GCCTTGCTGGC
BpuAI 1461 GAAGAC GAAGAC(2/6)
2095 GTCTTC
BsaBI 385 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3081 GATTATCATC
BsaWI 1810 ACCGGA [AT]^CCGG[AT]
2742 TCCGGA
BsiHKAI 212 GAGCTC G[AT]GC[AT]^C
1355 GTGCTC
BspMI 166 GCAGGT ACCTGC(4/8)
2157 GCAGGT
BsrDI 427 CATTGC GCAATG(2/0)
151 GCAATG
BstYI 1497 AGATCC [GA]^GATC[CT]
3462 AGATCC
EcoRI 1219 GAATTC G^AATTC
3311 GAATTC
EcoRV 268 GATATC GAT^ATC
2326 GATATC
FauI 918 GCGGG CCCGC(4/6)
2131 GCGGG
FbaI 882 TGATCA T^GATCA
3189 TGATCA
HaeII 39 AGCGCC [GA]GCGC^[CT]
611 GGCGCC
MamI 385 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3081 GATTATCATC
MflI 1497 AGATCC [GA]^GATC[CT]
3462 AGATCC
NciI 1334 CCGGG CC^[GC]GG
1864 CCCGG
NdeI 475 CATATG CA^TATG
1586 CATATG
ScaI 341 AGTACT AGT^ACT
3230 AGTACT
SphI 522 GCATGC GCATG^C
2968 GCATGC
TfiI 385 GATTC G^A[AT]TC
1240 GAATC
XhoII 1497 AGATCC [GA]^GATC[CT]
3462 AGATCC
XmnI 1379 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2048 GAAGTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BanI BanII
BbvI BciVI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI