################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII BalI BamHI BbvCI BcgI BfrI BglII BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HgaI HpaI KpnI KspI MfeI MluNI MscI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 3096 CGCG CG^CG AccIII 2742 TCCGGA T^CCGGA AcyI 611 GGCGCC G[GA]^CG[CT]C AhdI 895 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2869 CCTGAGG CC^T[ACGT]AGG AspEI 895 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 43 CCCGAG C^[CT]CG[GA]G AvrII 217 CCTAGG C^CTAGG BbeI 611 GGCGCC GGCGC^C BbrPI 2898 CACGTG CAC^GTG BlnI 217 CCTAGG C^CTAGG BlpI 697 GCTCAGC GC^T[ACGT]AGC Bpu1102I 697 GCTCAGC GC^T[ACGT]AGC BsaAI 2898 CACGTG [CT]AC^GT[GA] BsaHI 611 GGCGCC G[GA]^CG[CT]C BsaI 575 GGTCTC GGTCTC(1/5) BseAI 2742 TCCGGA T^CCGGA BsoBI 43 CCCGAG C^[CT]CG[GA]G BspEI 2742 TCCGGA T^CCGGA BspLU11I 933 ACATGT A^CATGT BsrFI 2015 GCCGGT [GA]^CCGG[CT] Bst1107I 2294 GTATAC GTA^TAC BstUI 3096 CGCG CG^CG BstZ17I 2294 GTATAC GTA^TAC Bsu36I 2869 CCTGAGG CC^T[ACGT]AGG CelII 697 GCTCAGC GC^T[ACGT]AGC Cfr10I 2015 GCCGGT [GA]^CCGG[CT] DraII 1431 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 2149 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 452 CCATGG C^C[GA][CT]GG EaeI 825 CGGCCA [CT]^GGCC[GA] Eam1105I 895 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2869 CCTGAGG CC^T[ACGT]AGG EcoNI 2520 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1431 AGGCCCT [GA]G^G[ACGT]CC[CT] Hin1I 611 GGCGCC G[GA]^CG[CT]C KasI 611 GGCGCC G^GCGCC MluI 3095 ACGCGT A^CGCGT MroI 2742 TCCGGA T^CCGGA MvnI 3096 CGCG CG^CG NarI 611 GGCGCC GG^CGCC NcoI 452 CCATGG C^CATGG PacI 1225 TTAATTAA TTAAT^TAA PflMI 841 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2898 CACGTG CAC^GTG PmlI 2898 CACGTG CAC^GTG SacI 212 GAGCTC GAGCT^C SexAI 3280 ACCTGGT A^CC[AT]GGT SfoI 611 GGCGCC GGC^GCC SmlI 925 CTTGAG C^T[CT][GA]AG SpeI 2122 ACTAGT A^CTAGT SspI 1983 AATATT AAT^ATT TaqI 1437 TCGA T^CGA TthHB8I 1437 TCGA T^CGA Van91I 841 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 933 ACATGT A^C[GA][CT]GT 3095 ACGCGT AlwNI 347 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1816 CAGCAGCTG Asp700I 1379 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2048 GAAGTAATTC AspHI 212 GAGCTC G[AT]GC[AT]^C 1355 GTGCTC BbsI 1461 GAAGAC GAAGAC(2/6) 2095 GTCTTC BclI 882 TGATCA T^GATCA 3189 TGATCA BcnI 1334 CCGGG CC^[GC]GG 1864 CCCGG BglI 1884 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3424 GCCTTGCTGGC BpuAI 1461 GAAGAC GAAGAC(2/6) 2095 GTCTTC BsaBI 385 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3081 GATTATCATC BsaWI 1810 ACCGGA [AT]^CCGG[AT] 2742 TCCGGA BsiHKAI 212 GAGCTC G[AT]GC[AT]^C 1355 GTGCTC BspMI 166 GCAGGT ACCTGC(4/8) 2157 GCAGGT BsrDI 427 CATTGC GCAATG(2/0) 151 GCAATG BstYI 1497 AGATCC [GA]^GATC[CT] 3462 AGATCC EcoRI 1219 GAATTC G^AATTC 3311 GAATTC EcoRV 268 GATATC GAT^ATC 2326 GATATC FauI 918 GCGGG CCCGC(4/6) 2131 GCGGG FbaI 882 TGATCA T^GATCA 3189 TGATCA HaeII 39 AGCGCC [GA]GCGC^[CT] 611 GGCGCC MamI 385 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3081 GATTATCATC MflI 1497 AGATCC [GA]^GATC[CT] 3462 AGATCC NciI 1334 CCGGG CC^[GC]GG 1864 CCCGG NdeI 475 CATATG CA^TATG 1586 CATATG ScaI 341 AGTACT AGT^ACT 3230 AGTACT SphI 522 GCATGC GCATG^C 2968 GCATGC TfiI 385 GATTC G^A[AT]TC 1240 GAATC XhoII 1497 AGATCC [GA]^GATC[CT] 3462 AGATCC XmnI 1379 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2048 GAAGTAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BanII BbvI BciVI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI