##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BciVI BclI BfrI
BlnI BsaBI BsmBI Bsp1407I BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CfoI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FbaI FseI
FspI HaeII HhaI HinP1I HpaI
KasI KpnI MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SspBI
SspI StuI Van91I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 2951 TCCGGA T^CCGGA
AcyI 173 GACGCC G[GA]^CG[CT]C
AlwNI 1098 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1631 GGGCCC GGGCC^C
AspI 1398 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 638 GGATCC G^GATCC
BbrPI 1555 CACGTG CAC^GTG
BbvCI 2047 CCTCAGC CCTCAGC(-5/-2)
BglI 1522 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1643 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1643 GCTCAGC GC^T[ACGT]AGC
BsaHI 173 GACGCC G[GA]^CG[CT]C
BsaI 2551 GGTCTC GGTCTC(1/5)
BseAI 2951 TCCGGA T^CCGGA
BsiEI 2060 CGACCG CG[GA][CT]^CG
BsiWI 1897 CGTACG C^GTACG
BspEI 2951 TCCGGA T^CCGGA
BsrFI 1783 ACCGGC [GA]^CCGG[CT]
BssSI 897 CTCGTG CACGAG(-5/-1)
BstAPI 2830 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1643 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1783 ACCGGC [GA]^CCGG[CT]
DraI 3354 TTTAAA TTT^AAA
DrdI 1395 GACGACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 38 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1416 GATATC GAT^ATC
Hin1I 173 GACGCC G[GA]^CG[CT]C
HindIII 1292 AAGCTT A^AGCTT
KspI 2817 CCGCGG CCGC^GG
MroI 2951 TCCGGA T^CCGGA
PaeR7I 426 CTCGAG C^TCGAG
PflFI 1398 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1555 CACGTG CAC^GTG
PmlI 1555 CACGTG CAC^GTG
SacI 471 GAGCTC GAGCT^C
SacII 2817 CCGCGG CCGC^GG
SmaI 361 CCCGGG CCC^GGG
SpeI 2867 ACTAGT A^CTAGT
SphI 1874 GCATGC GCATG^C
SwaI 3353 ATTTAAAT ATTT^AAAT
Tth111I 1398 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3126 TCTAGA T^CTAGA
XcmI 1852 CCAAGGGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 426 CTCGAG C^TCGAG
XmaCI 361 CCCGGG C^CCGGG
XmaI 361 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 2058 CGCG CG^CG
2818 CGCG
AcsI 299 GAATTT [GA]^AATT[CT]
3357 AAATTT
Alw44I 2254 GTGCAC G^TGCAC
2748 GTGCAC
ApaLI 2254 GTGCAC G^TGCAC
2748 GTGCAC
ApoI 299 GAATTT [GA]^AATT[CT]
3357 AAATTT
Asp700I 1948 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2138 GAAGCATTTC
BalI 1153 TGGCCA TGG^CCA
2119 TGGCCA
BanI 1353 GGCACC G^G[CT][GA]CC
1371 GGCACC
BcgI 866 CGACAACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
498 GCACAGTCGTCG
BglII 1437 AGATCT A^GATCT
3343 AGATCT
BsaAI 1555 CACGTG [CT]AC^GT[GA]
1899 TACGTG
BsaWI 1125 ACCGGA [AT]^CCGG[AT]
2951 TCCGGA
BsmI 3056 GAATGC GAATGC(1/-1)
2039 GCATTC
BspDI 882 ATCGAT AT^CGAT
1419 ATCGAT
BspHI 1006 TCATGA T^CATGA
3052 TCATGA
BstUI 2058 CGCG CG^CG
2818 CGCG
ClaI 882 ATCGAT AT^CGAT
1419 ATCGAT
DsaI 460 CCGTGG C^C[GA][CT]GG
2817 CCGCGG
FauI 1634 CCCGC CCCGC(4/6)
2375 GCGGG
HincII 170 GTTGAC GT[CT]^[GA]AC
1602 GTCAAC
HindII 170 GTTGAC GT[CT]^[GA]AC
1602 GTCAAC
MluNI 1153 TGGCCA TGG^CCA
2119 TGGCCA
MscI 1153 TGGCCA TGG^CCA
2119 TGGCCA
MvnI 2058 CGCG CG^CG
2818 CGCG
NspI 1874 GCATGC [GA]CATG^[CT]
2035 ACATGC
PpuMI 760 GGGACCT [GA]G^G[AT]CC[CT]
2087 GGGACCC
PvuII 1284 CAGCTG CAG^CTG
2666 CAGCTG
RcaI 1006 TCATGA T^CATGA
3052 TCATGA
SapI 468 GAAGAGC GCTCTTC(1/4)
1191 GAAGAGC
XmnI 1948 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2138 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII