##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BbeI BbrPI
BcgI BciVI BclI BfrI BsaAI
BsiEI BsiWI BsmBI BssHII BstBI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoT22I FbaI FseI
HindIII HpaI KasI KpnI KspI
MaeII MfeI MluI MseI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tru9I Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 801 TCCGGA T^CCGGA
AcsI 2951 GAATTC [GA]^AATT[CT]
AcyI 957 GGCGTC G[GA]^CG[CT]C
AflIII 83 ACATGT A^C[GA][CT]GT
Aor51HI 3234 AGCGCT AGC^GCT
ApoI 2951 GAATTC [GA]^AATT[CT]
AviII 1206 TGCGCA TGC^GCA
AvrII 2824 CCTAGG C^CTAGG
BbsI 1370 GAAGAC GAAGAC(2/6)
BlnI 2824 CCTAGG C^CTAGG
BpuAI 1370 GAAGAC GAAGAC(2/6)
BsaBI 2446 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 957 GGCGTC G[GA]^CG[CT]C
BsaWI 801 TCCGGA [AT]^CCGG[AT]
BseAI 801 TCCGGA T^CCGGA
Bsp1407I 1656 TGTACA T^GTACA
BspDI 172 ATCGAT AT^CGAT
BspEI 801 TCCGGA T^CCGGA
BspHI 215 TCATGA T^CATGA
BspLU11I 83 ACATGT A^CATGT
BsrDI 1687 CATTGC GCAATG(2/0)
BsrGI 1656 TGTACA T^GTACA
Bst1107I 2575 GTATAC GTA^TAC
BstEII 2828 GGTGACC G^GT[ACGT]ACC
BstPI 2828 GGTGACC G^GT[ACGT]ACC
BstXI 315 CCAGGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2575 GTATAC GTA^TAC
ClaI 172 ATCGAT AT^CGAT
DraIII 1620 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3234 AGCGCT AGC^GCT
EcoO65I 2828 GGTGACC G^GT[ACGT]ACC
EcoRI 2951 GAATTC G^AATTC
EcoRV 815 GATATC GAT^ATC
FspI 1206 TGCGCA TGC^GCA
HaeII 3234 AGCGCT [GA]GCGC^[CT]
Hin1I 957 GGCGTC G[GA]^CG[CT]C
HincII 914 GTCGAC GT[CT]^[GA]AC
HindII 914 GTCGAC GT[CT]^[GA]AC
MamI 2446 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 801 TCCGGA T^CCGGA
NaeI 344 GCCGGC GCC^GGC
NgoMIV 344 GCCGGC G^CCGGC
NheI 2863 GCTAGC G^CTAGC
RcaI 215 TCATGA T^CATGA
SalI 914 GTCGAC G^TCGAC
SspBI 1656 TGTACA T^GTACA
SspI 3399 AATATT AAT^ATT
TfiI 178 GAATC G^A[AT]TC
Tsp509I 2952 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 722 CCTGAGG CC^T[ACGT]AGG
1292 CCTGAGG
BamHI 165 GGATCC G^GATCC
718 GGATCC
BfaI 2825 CTAG C^TAG
2864 CTAG
BglII 2505 AGATCT A^GATCT
3105 AGATCT
BlpI 832 GCTGAGC GC^T[ACGT]AGC
2903 GCTGAGC
Bpu1102I 832 GCTGAGC GC^T[ACGT]AGC
2903 GCTGAGC
BsaI 2709 GAGACC GGTCTC(1/5)
2963 GAGACC
BsmI 775 GAATGC GAATGC(1/-1)
2563 GAATGC
BsrFI 344 GCCGGC [GA]^CCGG[CT]
2880 ACCGGC
BssSI 2300 CACGAG CACGAG(-5/-1)
3143 CTCGTG
Bsu36I 722 CCTGAGG CC^T[ACGT]AGG
1292 CCTGAGG
CelII 832 GCTGAGC GC^T[ACGT]AGC
2903 GCTGAGC
Cfr10I 344 GCCGGC [GA]^CCGG[CT]
2880 ACCGGC
Eco81I 722 CCTGAGG CC^T[ACGT]AGG
1292 CCTGAGG
HgaI 958 GCGTC GACGC(5/10)
2521 GCGTC
MaeI 2825 CTAG C^TAG
2864 CTAG
SacI 1020 GAGCTC GAGCT^C
1935 GAGCTC
SmaI 993 CCCGGG CCC^GGG
1245 CCCGGG
XmaCI 993 CCCGGG C^CCGGG
1245 CCCGGG
XmaI 993 CCCGGG C^CCGGG
1245 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII