##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII BbvCI BcgI BclI BfrI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BsrDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoRI EcoRV FbaI FseI
FspI HpaI KspI MamI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1568 GGTACC G^GTACC
AclI 252 AACGTT AA^CGTT
AcyI 1398 GGCGCC G[GA]^CG[CT]C
AocI 3067 CCTCAGG CC^T[ACGT]AGG
Asp700I 3570 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1568 GGTACC G^GTACC
BbeI 1398 GGCGCC GGCGC^C
BbrPI 1749 CACGTG CAC^GTG
BciVI 3743 GGATAC GTATCC(6/5)
BsaAI 1749 CACGTG [CT]AC^GT[GA]
BsaHI 1398 GGCGCC G[GA]^CG[CT]C
BsaI 3021 GGTCTC GGTCTC(1/5)
BsaWI 987 ACCGGA [AT]^CCGG[AT]
BsmBI 1174 GAGACG CGTCTC(1/5)
BsmI 810 GAATGC GAATGC(1/-1)
Bsp1407I 3478 TGTACA T^GTACA
BsrGI 3478 TGTACA T^GTACA
BstEII 108 GGTCACC G^GT[ACGT]ACC
BstPI 108 GGTCACC G^GT[ACGT]ACC
Bsu36I 3067 CCTCAGG CC^T[ACGT]AGG
DrdI 1350 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 486 CCATGG C^C[GA][CT]GG
EagI 3127 CGGCCG C^GGCCG
EclXI 3127 CGGCCG C^GGCCG
Eco52I 3127 CGGCCG C^GGCCG
Eco81I 3067 CCTCAGG CC^T[ACGT]AGG
EcoO65I 108 GGTCACC G^GT[ACGT]ACC
EcoT22I 3304 ATGCAT ATGCA^T
Hin1I 1398 GGCGCC G[GA]^CG[CT]C
HincII 2046 GTCAAC GT[CT]^[GA]AC
HindII 2046 GTCAAC GT[CT]^[GA]AC
KasI 1398 GGCGCC G^GCGCC
KpnI 1568 GGTACC GGTAC^C
NarI 1398 GGCGCC GG^CGCC
NcoI 486 CCATGG C^CATGG
NdeI 964 CATATG CA^TATG
NsiI 3304 ATGCAT ATGCA^T
PmaCI 1749 CACGTG CAC^GTG
PmlI 1749 CACGTG CAC^GTG
Psp1406I 252 AACGTT AA^CGTT
SacI 2910 GAGCTC GAGCT^C
SfoI 1398 GGCGCC GGC^GCC
SgrAI 2547 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2715 CTCAAG C^T[CT][GA]AG
SphI 3734 GCATGC GCATG^C
SspBI 3478 TGTACA T^GTACA
SspI 3825 AATATT AAT^ATT
XbaI 177 TCTAGA T^CTAGA
XmnI 3570 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1359 GTCTAC GT^[AC][GT]AC
2004 GTAGAC
BamHI 1855 GGATCC G^GATCC
2681 GGATCC
BglI 692 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2499 GCCCTGGGGGC
BglII 1186 AGATCT A^GATCT
2163 AGATCT
BlpI 1339 GCTCAGC GC^T[ACGT]AGC
3142 GCTCAGC
Bpu1102I 1339 GCTCAGC GC^T[ACGT]AGC
3142 GCTCAGC
BspLU11I 2105 ACATGT A^CATGT
3224 ACATGT
BstAPI 1385 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2897 GCACCCACTGC
CelII 1339 GCTCAGC GC^T[ACGT]AGC
3142 GCTCAGC
EcoNI 1679 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3611 CCTGCTTCAGG
HindIII 1163 AAGCTT A^AGCTT
3461 AAGCTT
NaeI 2427 GCCGGC GCC^GGC
2548 GCCGGC
NgoMIV 2427 GCCGGC G^CCGGC
2548 GCCGGC
SexAI 1690 ACCAGGT A^CC[AT]GGT
2203 ACCTGGT
TaqI 1061 TCGA T^CGA
2558 TCGA
TfiI 127 GATTC G^A[AT]TC
394 GATTC
TthHB8I 1061 TCGA T^CGA
2558 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I Van91I XcmI
XhoII