################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII Alw44I ApaI ApaLI AscI Asp718I AspI AvrII BanI BbeI BbrPI BbsI BcgI BclI BfrI BlnI BlpI Bpu1102I BpuAI BseAI BsiEI BsiWI BsmI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI HpaI KasI KpnI KspI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PmaCI PmeI PmlI Psp1406I PvuI RcaI SacII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AgeI 1724 ACCGGT A^CCGGT AocI 1461 CCTGAGG CC^T[ACGT]AGG Aor51HI 2675 AGCGCT AGC^GCT AseI 2490 ATTAAT AT^TAAT AsnI 2490 ATTAAT AT^TAAT BciVI 1272 GTATCC GTATCC(6/5) BsaAI 232 TACGTG [CT]AC^GT[GA] BsaWI 1724 ACCGGT [AT]^CCGG[AT] BsgI 331 GTGCAG GTGCAG(16/14) Bsp1407I 3155 TGTACA T^GTACA BspDI 1612 ATCGAT AT^CGAT BspMI 137 ACCTGC ACCTGC(4/8) BsrDI 3214 GCAATG GCAATG(2/0) BsrFI 1724 ACCGGT [GA]^CCGG[CT] BsrGI 3155 TGTACA T^GTACA BstXI 536 CCATCGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1461 CCTGAGG CC^T[ACGT]AGG Cfr10I 1724 ACCGGT [GA]^CCGG[CT] ClaI 1612 ATCGAT AT^CGAT CpoI 1655 CGGACCG CG^G[AT]CCG DraI 2647 TTTAAA TTT^AAA Eco47III 2675 AGCGCT AGC^GCT Eco81I 1461 CCTGAGG CC^T[ACGT]AGG EcoNI 375 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 797 GAATTC G^AATTC HaeII 2675 AGCGCT [GA]GCGC^[CT] HindIII 833 AAGCTT A^AGCTT MfeI 1943 CAATTG C^AATTG MunI 1943 CAATTG C^AATTG PaeR7I 268 CTCGAG C^TCGAG PinAI 1724 ACCGGT A^CCGGT PshAI 3243 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2490 ATTAAT AT^TAAT PstI 64 CTGCAG CTGCA^G RsrII 1655 CGGACCG CG^G[AT]CCG ScaI 756 AGTACT AGT^ACT SfcI 64 CTGCAG C^T[GA][CT]AG SfiI 2343 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 443 CCCGGG CCC^GGG SphI 59 GCATGC GCATG^C SspBI 3155 TGTACA T^GTACA XhoI 268 CTCGAG C^TCGAG XmaCI 443 CCCGGG C^CCGGG XmaI 443 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 934 GACGTC GACGT^C 1579 GACGTC AccII 2364 CGCG CG^CG 2939 CGCG AhdI 702 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2254 GACACACGGTC Asp700I 739 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3221 GAAATACTTC AspEI 702 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2254 GACACACGGTC AviII 1241 TGCGCA TGC^GCA 2389 TGCGCA BamHI 2383 GGATCC G^GATCC 2830 GGATCC BbvCI 1686 CCTCAGC CCTCAGC(-5/-2) 90 GCTGAGG BglI 2344 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2801 GCCCAGAGGGC BglII 2144 AGATCT A^GATCT 2842 AGATCT BsaBI 531 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1188 GATCCGCATC BsaI 1988 GAGACC GGTCTC(1/5) 2606 GGTCTC BsmBI 932 GAGACG CGTCTC(1/5) 2095 GAGACG BssSI 2092 CACGAG CACGAG(-5/-1) 1477 CTCGTG BstUI 2364 CGCG CG^CG 2939 CGCG Eam1105I 702 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2254 GACACACGGTC FspI 1241 TGCGCA TGC^GCA 2389 TGCGCA MamI 531 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1188 GATCCGCATC MslI 514 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 537 CATCGTTATG MvnI 2364 CGCG CG^CG 2939 CGCG PvuII 2035 CAGCTG CAG^CTG 2870 CAGCTG SmlI 268 CTCGAG C^T[CT][GA]AG 2557 CTTGAG StuI 1016 AGGCCT AGG^CCT 2933 AGGCCT XmnI 739 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3221 GAAATACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII