##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII Alw44I ApaI ApaLI AscI
Asp718I AspI AvrII BanI BbeI
BbrPI BbsI BcgI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BseAI
BsiEI BsiWI BsmI BspEI BspHI
BspLU11I BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII DrdI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI SacII SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AgeI 1724 ACCGGT A^CCGGT
AocI 1461 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2675 AGCGCT AGC^GCT
AseI 2490 ATTAAT AT^TAAT
AsnI 2490 ATTAAT AT^TAAT
BciVI 1272 GTATCC GTATCC(6/5)
BsaAI 232 TACGTG [CT]AC^GT[GA]
BsaWI 1724 ACCGGT [AT]^CCGG[AT]
BsgI 331 GTGCAG GTGCAG(16/14)
Bsp1407I 3155 TGTACA T^GTACA
BspDI 1612 ATCGAT AT^CGAT
BspMI 137 ACCTGC ACCTGC(4/8)
BsrDI 3214 GCAATG GCAATG(2/0)
BsrFI 1724 ACCGGT [GA]^CCGG[CT]
BsrGI 3155 TGTACA T^GTACA
BstXI 536 CCATCGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1461 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1724 ACCGGT [GA]^CCGG[CT]
ClaI 1612 ATCGAT AT^CGAT
CpoI 1655 CGGACCG CG^G[AT]CCG
DraI 2647 TTTAAA TTT^AAA
Eco47III 2675 AGCGCT AGC^GCT
Eco81I 1461 CCTGAGG CC^T[ACGT]AGG
EcoNI 375 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 797 GAATTC G^AATTC
HaeII 2675 AGCGCT [GA]GCGC^[CT]
HindIII 833 AAGCTT A^AGCTT
MfeI 1943 CAATTG C^AATTG
MunI 1943 CAATTG C^AATTG
PaeR7I 268 CTCGAG C^TCGAG
PinAI 1724 ACCGGT A^CCGGT
PshAI 3243 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2490 ATTAAT AT^TAAT
PstI 64 CTGCAG CTGCA^G
RsrII 1655 CGGACCG CG^G[AT]CCG
ScaI 756 AGTACT AGT^ACT
SfcI 64 CTGCAG C^T[GA][CT]AG
SfiI 2343 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 443 CCCGGG CCC^GGG
SphI 59 GCATGC GCATG^C
SspBI 3155 TGTACA T^GTACA
XhoI 268 CTCGAG C^TCGAG
XmaCI 443 CCCGGG C^CCGGG
XmaI 443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 934 GACGTC GACGT^C
1579 GACGTC
AccII 2364 CGCG CG^CG
2939 CGCG
AhdI 702 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2254 GACACACGGTC
Asp700I 739 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3221 GAAATACTTC
AspEI 702 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2254 GACACACGGTC
AviII 1241 TGCGCA TGC^GCA
2389 TGCGCA
BamHI 2383 GGATCC G^GATCC
2830 GGATCC
BbvCI 1686 CCTCAGC CCTCAGC(-5/-2)
90 GCTGAGG
BglI 2344 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2801 GCCCAGAGGGC
BglII 2144 AGATCT A^GATCT
2842 AGATCT
BsaBI 531 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1188 GATCCGCATC
BsaI 1988 GAGACC GGTCTC(1/5)
2606 GGTCTC
BsmBI 932 GAGACG CGTCTC(1/5)
2095 GAGACG
BssSI 2092 CACGAG CACGAG(-5/-1)
1477 CTCGTG
BstUI 2364 CGCG CG^CG
2939 CGCG
Eam1105I 702 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2254 GACACACGGTC
FspI 1241 TGCGCA TGC^GCA
2389 TGCGCA
MamI 531 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1188 GATCCGCATC
MslI 514 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
537 CATCGTTATG
MvnI 2364 CGCG CG^CG
2939 CGCG
PvuII 2035 CAGCTG CAG^CTG
2870 CAGCTG
SmlI 268 CTCGAG C^T[CT][GA]AG
2557 CTTGAG
StuI 1016 AGGCCT AGG^CCT
2933 AGGCCT
XmnI 739 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3221 GAAATACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII