################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AccIII AclI AflII AocI Aor51HI ApaI AscI AseI AsnI AviII AvrII BamHI BanII BbrPI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoT22I FseI FspI HpaI KspI MluI MroI NciI NdeI NheI NotI NsiI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 2779 GGTACC G^GTACC AgeI 3260 ACCGGT A^CCGGT AhdI 2391 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 2779 GGTACC G^GTACC AspEI 2391 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 3112 CCTCAGC CCTCAGC(-5/-2) BciVI 1426 GGATAC GTATCC(6/5) BclI 612 TGATCA T^GATCA BglII 1256 AGATCT A^GATCT BsaBI 607 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3260 ACCGGT [AT]^CCGG[AT] BstEII 82 GGTCACC G^GT[ACGT]ACC BstPI 82 GGTCACC G^GT[ACGT]ACC DraIII 2324 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1069 GACCGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2391 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 82 GGTCACC G^GT[ACGT]ACC EcoRV 2005 GATATC GAT^ATC FauI 3 CCCGC CCCGC(4/6) FbaI 612 TGATCA T^GATCA HindIII 2440 AAGCTT A^AGCTT KpnI 2779 GGTACC GGTAC^C MamI 607 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2731 CAATTG C^AATTG MunI 2731 CAATTG C^AATTG NaeI 1472 GCCGGC GCC^GGC NgoMIV 1472 GCCGGC G^CCGGC NruI 1827 TCGCGA TCG^CGA PaeR7I 1397 CTCGAG C^TCGAG PinAI 3260 ACCGGT A^CCGGT PshAI 754 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1652 CTGCAG CTGCA^G SphI 826 GCATGC GCATG^C SspI 944 AATATT AAT^ATT StuI 3017 AGGCCT AGG^CCT SwaI 809 ATTTAAAT ATTT^AAAT TaqI 1398 TCGA T^CGA TthHB8I 1398 TCGA T^CGA XcmI 368 CCACTGCTACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1397 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccI 1175 GTAGAC GT^[AC][GT]AC 2345 GTCTAC AccII 5 CGCG CG^CG 1828 CGCG AflIII 1994 ACATGT A^C[GA][CT]GT 2929 ACATGT Alw44I 1513 GTGCAC G^TGCAC 2539 GTGCAC ApaLI 1513 GTGCAC G^TGCAC 2539 GTGCAC Asp700I 2862 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3015 GAAGGCCTTC AspI 496 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2966 GACTCTGTC BalI 123 TGGCCA TGG^CCA 2109 TGGCCA BbeI 14 GGCGCC GGCGC^C 1796 GGCGCC BsgI 1314 CTGCAC GTGCAG(16/14) 3209 CTGCAC BspLU11I 1994 ACATGT A^CATGT 2929 ACATGT BsrFI 1472 GCCGGC [GA]^CCGG[CT] 3260 ACCGGT BstAPI 901 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 997 GCACTGATTGC BstUI 5 CGCG CG^CG 1828 CGCG Cfr10I 1472 GCCGGC [GA]^CCGG[CT] 3260 ACCGGT DsaI 2106 CCATGG C^C[GA][CT]GG 2653 CCATGG EaeI 123 TGGCCA [CT]^GGCC[GA] 2109 TGGCCA EcoNI 31 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 303 CCTTGGTCAGG EcoRI 2183 GAATTC G^AATTC 2290 GAATTC HaeII 14 GGCGCC [GA]GCGC^[CT] 1796 GGCGCC HgaI 1288 GACGC GACGC(5/10) 2482 GCGTC HincII 427 GTTGAC GT[CT]^[GA]AC 2963 GTTGAC HindII 427 GTTGAC GT[CT]^[GA]AC 2963 GTTGAC KasI 14 GGCGCC G^GCGCC 1796 GGCGCC MaeII 1119 ACGT A^CGT 2477 ACGT MluNI 123 TGGCCA TGG^CCA 2109 TGGCCA MscI 123 TGGCCA TGG^CCA 2109 TGGCCA MvnI 5 CGCG CG^CG 1828 CGCG NarI 14 GGCGCC GG^CGCC 1796 GGCGCC NcoI 2106 CCATGG C^CATGG 2653 CCATGG PflFI 496 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2966 GACTCTGTC PflMI 115 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 345 CCACCTATTGG SfaNI 778 GATGC GCATC(5/9) 392 GCATC SfoI 14 GGCGCC GGC^GCC 1796 GGCGCC SmlI 328 CTTGAG C^T[CT][GA]AG 1397 CTCGAG Tth111I 496 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2966 GACTCTGTC Van91I 115 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 345 CCACCTATTGG XmnI 2862 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3015 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BbsI BbvI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XhoII