##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AviII       AvrII       BamHI       BanII       BbrPI       
BcgI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoT22I     FseI        FspI        
HpaI        KspI        MluI        MroI        NciI        
NdeI        NheI        NotI        NsiI        NspV        
PacI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       2779  GGTACC               G^GTACC
AgeI         3260  ACCGGT               A^CCGGT
AhdI         2391  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      2779  GGTACC               G^GTACC
AspEI        2391  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI        3112  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1426  GGATAC               GTATCC(6/5)
BclI          612  TGATCA               T^GATCA
BglII        1256  AGATCT               A^GATCT
BsaBI         607  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3260  ACCGGT               [AT]^CCGG[AT]
BstEII         82  GGTCACC              G^GT[ACGT]ACC
BstPI          82  GGTCACC              G^GT[ACGT]ACC
DraIII       2324  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1069  GACCGACTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2391  GACAGCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I        82  GGTCACC              G^GT[ACGT]ACC
EcoRV        2005  GATATC               GAT^ATC
FauI            3  CCCGC                CCCGC(4/6)
FbaI          612  TGATCA               T^GATCA
HindIII      2440  AAGCTT               A^AGCTT
KpnI         2779  GGTACC               GGTAC^C
MamI          607  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2731  CAATTG               C^AATTG
MunI         2731  CAATTG               C^AATTG
NaeI         1472  GCCGGC               GCC^GGC
NgoMIV       1472  GCCGGC               G^CCGGC
NruI         1827  TCGCGA               TCG^CGA
PaeR7I       1397  CTCGAG               C^TCGAG
PinAI        3260  ACCGGT               A^CCGGT
PshAI         754  GACGAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1652  CTGCAG               CTGCA^G
SphI          826  GCATGC               GCATG^C
SspI          944  AATATT               AAT^ATT
StuI         3017  AGGCCT               AGG^CCT
SwaI          809  ATTTAAAT             ATTT^AAAT
TaqI         1398  TCGA                 T^CGA
TthHB8I      1398  TCGA                 T^CGA
XcmI          368  CCACTGCTACTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1397  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AccI         1175  GTAGAC               GT^[AC][GT]AC
             2345  GTCTAC              
AccII           5  CGCG                 CG^CG
             1828  CGCG                
AflIII       1994  ACATGT               A^C[GA][CT]GT
             2929  ACATGT              
Alw44I       1513  GTGCAC               G^TGCAC
             2539  GTGCAC              
ApaLI        1513  GTGCAC               G^TGCAC
             2539  GTGCAC              
Asp700I      2862  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3015  GAAGGCCTTC          
AspI          496  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2966  GACTCTGTC           
BalI          123  TGGCCA               TGG^CCA
             2109  TGGCCA              
BbeI           14  GGCGCC               GGCGC^C
             1796  GGCGCC              
BsgI         1314  CTGCAC               GTGCAG(16/14)
             3209  CTGCAC              
BspLU11I     1994  ACATGT               A^CATGT
             2929  ACATGT              
BsrFI        1472  GCCGGC               [GA]^CCGG[CT]
             3260  ACCGGT              
BstAPI        901  GCAGGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              997  GCACTGATTGC         
BstUI           5  CGCG                 CG^CG
             1828  CGCG                
Cfr10I       1472  GCCGGC               [GA]^CCGG[CT]
             3260  ACCGGT              
DsaI         2106  CCATGG               C^C[GA][CT]GG
             2653  CCATGG              
EaeI          123  TGGCCA               [CT]^GGCC[GA]
             2109  TGGCCA              
EcoNI          31  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              303  CCTTGGTCAGG         
EcoRI        2183  GAATTC               G^AATTC
             2290  GAATTC              
HaeII          14  GGCGCC               [GA]GCGC^[CT]
             1796  GGCGCC              
HgaI         1288  GACGC                GACGC(5/10)
             2482  GCGTC               
HincII        427  GTTGAC               GT[CT]^[GA]AC
             2963  GTTGAC              
HindII        427  GTTGAC               GT[CT]^[GA]AC
             2963  GTTGAC              
KasI           14  GGCGCC               G^GCGCC
             1796  GGCGCC              
MaeII        1119  ACGT                 A^CGT
             2477  ACGT                
MluNI         123  TGGCCA               TGG^CCA
             2109  TGGCCA              
MscI          123  TGGCCA               TGG^CCA
             2109  TGGCCA              
MvnI            5  CGCG                 CG^CG
             1828  CGCG                
NarI           14  GGCGCC               GG^CGCC
             1796  GGCGCC              
NcoI         2106  CCATGG               C^CATGG
             2653  CCATGG              
PflFI         496  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2966  GACTCTGTC           
PflMI         115  CCAGTGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              345  CCACCTATTGG         
SfaNI         778  GATGC                GCATC(5/9)
              392  GCATC               
SfoI           14  GGCGCC               GGC^GCC
             1796  GGCGCC              
SmlI          328  CTTGAG               C^T[CT][GA]AG
             1397  CTCGAG              
Tth111I       496  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2966  GACTCTGTC           
Van91I        115  CCAGTGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              345  CCACCTATTGG         
XmnI         2862  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3015  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BanI        BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspHI       BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XbaI        
XhoII