##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AflII AocI
Aor51HI ApaI AscI AseI AsnI
AviII AvrII BamHI BanII BbrPI
BcgI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoT22I FseI FspI
HpaI KspI MluI MroI NciI
NdeI NheI NotI NsiI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2779 GGTACC G^GTACC
AgeI 3260 ACCGGT A^CCGGT
AhdI 2391 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2779 GGTACC G^GTACC
AspEI 2391 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 3112 CCTCAGC CCTCAGC(-5/-2)
BciVI 1426 GGATAC GTATCC(6/5)
BclI 612 TGATCA T^GATCA
BglII 1256 AGATCT A^GATCT
BsaBI 607 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3260 ACCGGT [AT]^CCGG[AT]
BstEII 82 GGTCACC G^GT[ACGT]ACC
BstPI 82 GGTCACC G^GT[ACGT]ACC
DraIII 2324 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1069 GACCGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2391 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 82 GGTCACC G^GT[ACGT]ACC
EcoRV 2005 GATATC GAT^ATC
FauI 3 CCCGC CCCGC(4/6)
FbaI 612 TGATCA T^GATCA
HindIII 2440 AAGCTT A^AGCTT
KpnI 2779 GGTACC GGTAC^C
MamI 607 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2731 CAATTG C^AATTG
MunI 2731 CAATTG C^AATTG
NaeI 1472 GCCGGC GCC^GGC
NgoMIV 1472 GCCGGC G^CCGGC
NruI 1827 TCGCGA TCG^CGA
PaeR7I 1397 CTCGAG C^TCGAG
PinAI 3260 ACCGGT A^CCGGT
PshAI 754 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1652 CTGCAG CTGCA^G
SphI 826 GCATGC GCATG^C
SspI 944 AATATT AAT^ATT
StuI 3017 AGGCCT AGG^CCT
SwaI 809 ATTTAAAT ATTT^AAAT
TaqI 1398 TCGA T^CGA
TthHB8I 1398 TCGA T^CGA
XcmI 368 CCACTGCTACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1397 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1175 GTAGAC GT^[AC][GT]AC
2345 GTCTAC
AccII 5 CGCG CG^CG
1828 CGCG
AflIII 1994 ACATGT A^C[GA][CT]GT
2929 ACATGT
Alw44I 1513 GTGCAC G^TGCAC
2539 GTGCAC
ApaLI 1513 GTGCAC G^TGCAC
2539 GTGCAC
Asp700I 2862 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3015 GAAGGCCTTC
AspI 496 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2966 GACTCTGTC
BalI 123 TGGCCA TGG^CCA
2109 TGGCCA
BbeI 14 GGCGCC GGCGC^C
1796 GGCGCC
BsgI 1314 CTGCAC GTGCAG(16/14)
3209 CTGCAC
BspLU11I 1994 ACATGT A^CATGT
2929 ACATGT
BsrFI 1472 GCCGGC [GA]^CCGG[CT]
3260 ACCGGT
BstAPI 901 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
997 GCACTGATTGC
BstUI 5 CGCG CG^CG
1828 CGCG
Cfr10I 1472 GCCGGC [GA]^CCGG[CT]
3260 ACCGGT
DsaI 2106 CCATGG C^C[GA][CT]GG
2653 CCATGG
EaeI 123 TGGCCA [CT]^GGCC[GA]
2109 TGGCCA
EcoNI 31 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
303 CCTTGGTCAGG
EcoRI 2183 GAATTC G^AATTC
2290 GAATTC
HaeII 14 GGCGCC [GA]GCGC^[CT]
1796 GGCGCC
HgaI 1288 GACGC GACGC(5/10)
2482 GCGTC
HincII 427 GTTGAC GT[CT]^[GA]AC
2963 GTTGAC
HindII 427 GTTGAC GT[CT]^[GA]AC
2963 GTTGAC
KasI 14 GGCGCC G^GCGCC
1796 GGCGCC
MaeII 1119 ACGT A^CGT
2477 ACGT
MluNI 123 TGGCCA TGG^CCA
2109 TGGCCA
MscI 123 TGGCCA TGG^CCA
2109 TGGCCA
MvnI 5 CGCG CG^CG
1828 CGCG
NarI 14 GGCGCC GG^CGCC
1796 GGCGCC
NcoI 2106 CCATGG C^CATGG
2653 CCATGG
PflFI 496 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2966 GACTCTGTC
PflMI 115 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
345 CCACCTATTGG
SfaNI 778 GATGC GCATC(5/9)
392 GCATC
SfoI 14 GGCGCC GGC^GCC
1796 GGCGCC
SmlI 328 CTTGAG C^T[CT][GA]AG
1397 CTCGAG
Tth111I 496 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2966 GACTCTGTC
Van91I 115 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
345 CCACCTATTGG
XmnI 2862 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3015 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XhoII