##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AviII BbrPI BcgI
BclI BfrI BsaAI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII BssSI Bst1107I BstZ17I
ClaI CpoI DrdI Eam1105I Eco47III
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI MamI MfeI
MluI MroI MunI NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
ScaI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1390 GACGTC GACGT^C
Acc65I 1861 GGTACC G^GTACC
AcsI 1337 GAATTC [GA]^AATT[CT]
ApoI 1337 GAATTC [GA]^AATT[CT]
Asp700I 2544 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1861 GGTACC G^GTACC
AvrII 1942 CCTAGG C^CTAGG
BamHI 2618 GGATCC G^GATCC
BbeI 1570 GGCGCC GGCGC^C
BbvCI 2321 CCTCAGC CCTCAGC(-5/-2)
BglI 2725 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1942 CCTAGG C^CTAGG
BsgI 1543 CTGCAC GTGCAG(16/14)
BsiEI 1305 CGGCCG CG[GA][CT]^CG
BsmI 2568 GCATTC GAATGC(1/-1)
BspHI 2505 TCATGA T^CATGA
BsrDI 2034 GCAATG GCAATG(2/0)
BstBI 1255 TTCGAA TT^CGAA
DraIII 2950 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1305 CGGCCG C^GGCCG
EclXI 1305 CGGCCG C^GGCCG
Eco52I 1305 CGGCCG C^GGCCG
EcoRI 1337 GAATTC G^AATTC
HgaI 350 GCGTC GACGC(5/10)
KasI 1570 GGCGCC G^GCGCC
KpnI 1861 GGTACC GGTAC^C
KspI 1302 CCGCGG CCGC^GG
NaeI 1236 GCCGGC GCC^GGC
NarI 1570 GGCGCC GG^CGCC
NgoMIV 1236 GCCGGC G^CCGGC
NspV 1255 TTCGAA TT^CGAA
PaeR7I 2194 CTCGAG C^TCGAG
RcaI 2505 TCATGA T^CATGA
SacII 1302 CCGCGG CCGC^GG
SfiI 2724 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1570 GGCGCC GGC^GCC
SfuI 1255 TTCGAA TT^CGAA
XbaI 3392 TCTAGA T^CTAGA
XhoI 2194 CTCGAG C^TCGAG
XmnI 2544 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1390 GACGTC G[GA]^CG[CT]C
1570 GGCGCC
Alw44I 1123 GTGCAC G^TGCAC
2421 GTGCAC
AocI 1069 CCTGAGG CC^T[ACGT]AGG
1762 CCTCAGG
ApaLI 1123 GTGCAC G^TGCAC
2421 GTGCAC
AspI 1786 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2442 GACTGAGTC
BciVI 302 GTATCC GTATCC(6/5)
577 GTATCC
BglII 92 AGATCT A^GATCT
1250 AGATCT
BsaHI 1390 GACGTC G[GA]^CG[CT]C
1570 GGCGCC
BsmBI 2024 CGTCTC CGTCTC(1/5)
1492 GAGACG
Bsp1407I 74 TGTACA T^GTACA
962 TGTACA
BspMI 1541 ACCTGC ACCTGC(4/8)
2558 ACCTGC
BsrGI 74 TGTACA T^GTACA
962 TGTACA
BstAPI 1478 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3331 GCAGCTTCTGC
BstEII 241 GGTTACC G^GT[ACGT]ACC
2984 GGTCACC
BstPI 241 GGTTACC G^GT[ACGT]ACC
2984 GGTCACC
BstXI 513 CCATGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2287 CCAGGGAAATGG
Bsu36I 1069 CCTGAGG CC^T[ACGT]AGG
1762 CCTCAGG
DraI 885 TTTAAA TTT^AAA
1429 TTTAAA
Eco81I 1069 CCTGAGG CC^T[ACGT]AGG
1762 CCTCAGG
EcoO65I 241 GGTTACC G^GT[ACGT]ACC
2984 GGTCACC
Hin1I 1390 GACGTC G[GA]^CG[CT]C
1570 GGCGCC
MslI 514 CATGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2119 CACCTTTGTG
NdeI 49 CATATG CA^TATG
3368 CATATG
PflFI 1786 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2442 GACTGAGTC
PstI 838 CTGCAG CTGCA^G
2313 CTGCAG
SexAI 2079 ACCTGGT A^CC[AT]GGT
2951 ACCAGGT
SphI 915 GCATGC GCATG^C
1358 GCATGC
SspBI 74 TGTACA T^GTACA
962 TGTACA
TfiI 38 GAATC G^A[AT]TC
1443 GAATC
Tsp509I 806 AATT ^AATT
1338 AATT
Tth111I 1786 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2442 GACTGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII