################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AccI AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AviII BbrPI BcgI BclI BfrI BsaAI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DrdI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI MamI MfeI MluI MroI MunI NheI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 1390 GACGTC GACGT^C Acc65I 1861 GGTACC G^GTACC AcsI 1337 GAATTC [GA]^AATT[CT] ApoI 1337 GAATTC [GA]^AATT[CT] Asp700I 2544 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1861 GGTACC G^GTACC AvrII 1942 CCTAGG C^CTAGG BamHI 2618 GGATCC G^GATCC BbeI 1570 GGCGCC GGCGC^C BbvCI 2321 CCTCAGC CCTCAGC(-5/-2) BglI 2725 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1942 CCTAGG C^CTAGG BsgI 1543 CTGCAC GTGCAG(16/14) BsiEI 1305 CGGCCG CG[GA][CT]^CG BsmI 2568 GCATTC GAATGC(1/-1) BspHI 2505 TCATGA T^CATGA BsrDI 2034 GCAATG GCAATG(2/0) BstBI 1255 TTCGAA TT^CGAA DraIII 2950 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1305 CGGCCG C^GGCCG EclXI 1305 CGGCCG C^GGCCG Eco52I 1305 CGGCCG C^GGCCG EcoRI 1337 GAATTC G^AATTC HgaI 350 GCGTC GACGC(5/10) KasI 1570 GGCGCC G^GCGCC KpnI 1861 GGTACC GGTAC^C KspI 1302 CCGCGG CCGC^GG NaeI 1236 GCCGGC GCC^GGC NarI 1570 GGCGCC GG^CGCC NgoMIV 1236 GCCGGC G^CCGGC NspV 1255 TTCGAA TT^CGAA PaeR7I 2194 CTCGAG C^TCGAG RcaI 2505 TCATGA T^CATGA SacII 1302 CCGCGG CCGC^GG SfiI 2724 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1570 GGCGCC GGC^GCC SfuI 1255 TTCGAA TT^CGAA XbaI 3392 TCTAGA T^CTAGA XhoI 2194 CTCGAG C^TCGAG XmnI 2544 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 1390 GACGTC G[GA]^CG[CT]C 1570 GGCGCC Alw44I 1123 GTGCAC G^TGCAC 2421 GTGCAC AocI 1069 CCTGAGG CC^T[ACGT]AGG 1762 CCTCAGG ApaLI 1123 GTGCAC G^TGCAC 2421 GTGCAC AspI 1786 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2442 GACTGAGTC BciVI 302 GTATCC GTATCC(6/5) 577 GTATCC BglII 92 AGATCT A^GATCT 1250 AGATCT BsaHI 1390 GACGTC G[GA]^CG[CT]C 1570 GGCGCC BsmBI 2024 CGTCTC CGTCTC(1/5) 1492 GAGACG Bsp1407I 74 TGTACA T^GTACA 962 TGTACA BspMI 1541 ACCTGC ACCTGC(4/8) 2558 ACCTGC BsrGI 74 TGTACA T^GTACA 962 TGTACA BstAPI 1478 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3331 GCAGCTTCTGC BstEII 241 GGTTACC G^GT[ACGT]ACC 2984 GGTCACC BstPI 241 GGTTACC G^GT[ACGT]ACC 2984 GGTCACC BstXI 513 CCATGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2287 CCAGGGAAATGG Bsu36I 1069 CCTGAGG CC^T[ACGT]AGG 1762 CCTCAGG DraI 885 TTTAAA TTT^AAA 1429 TTTAAA Eco81I 1069 CCTGAGG CC^T[ACGT]AGG 1762 CCTCAGG EcoO65I 241 GGTTACC G^GT[ACGT]ACC 2984 GGTCACC Hin1I 1390 GACGTC G[GA]^CG[CT]C 1570 GGCGCC MslI 514 CATGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2119 CACCTTTGTG NdeI 49 CATATG CA^TATG 3368 CATATG PflFI 1786 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2442 GACTGAGTC PstI 838 CTGCAG CTGCA^G 2313 CTGCAG SexAI 2079 ACCTGGT A^CC[AT]GGT 2951 ACCAGGT SphI 915 GCATGC GCATG^C 1358 GCATGC SspBI 74 TGTACA T^GTACA 962 TGTACA TfiI 38 GAATC G^A[AT]TC 1443 GAATC Tsp509I 806 AATT ^AATT 1338 AATT Tth111I 1786 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2442 GACTGAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII