##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI BbeI BcgI BciVI BsaBI
BsaHI BsaWI BseAI BsiEI BsmI
BspDI BspEI BspMI BssHII BstEII
BstPI ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FseI Hin1I HindIII
HpaI KasI KspI MamI MluI
MroI NaeI NarI NcoI NgoMIV
NotI NruI PacI PaeR7I PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3049 GGTACC G^GTACC
AflIII 1661 ACATGT A^C[GA][CT]GT
Alw44I 3066 GTGCAC G^TGCAC
ApaLI 3066 GTGCAC G^TGCAC
Asp700I 2970 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3049 GGTACC G^GTACC
AspI 189 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3025 CCCGAG C^[CT]CG[GA]G
AviII 3008 TGCGCA TGC^GCA
AvrII 529 CCTAGG C^CTAGG
BamHI 1074 GGATCC G^GATCC
BbrPI 1069 CACGTG CAC^GTG
BbsI 1188 GTCTTC GAAGAC(2/6)
BbvCI 2127 GCTGAGG CCTCAGC(-5/-2)
BcnI 920 CCGGG CC^[GC]GG
BglI 3144 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 529 CCTAGG C^CTAGG
BlpI 938 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 938 GCTGAGC GC^T[ACGT]AGC
BpuAI 1188 GTCTTC GAAGAC(2/6)
BsiWI 1686 CGTACG C^GTACG
BsmBI 3362 CGTCTC CGTCTC(1/5)
BsoBI 3025 CCCGAG C^[CT]CG[GA]G
BspLU11I 1661 ACATGT A^CATGT
BsrDI 1895 CATTGC GCAATG(2/0)
BsrFI 1945 ACCGGC [GA]^CCGG[CT]
BssSI 2709 CTCGTG CACGAG(-5/-1)
Bst1107I 1508 GTATAC GTA^TAC
BstBI 2977 TTCGAA TT^CGAA
BstZ17I 1508 GTATAC GTA^TAC
CelII 938 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1945 ACCGGC [GA]^CCGG[CT]
DraI 159 TTTAAA TTT^AAA
DrdI 1469 GACCTAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3008 TGCGCA TGC^GCA
HaeII 1976 AGCGCC [GA]GCGC^[CT]
KpnI 3049 GGTACC GGTAC^C
MfeI 3634 CAATTG C^AATTG
MunI 3634 CAATTG C^AATTG
NciI 920 CCGGG CC^[GC]GG
NdeI 3600 CATATG CA^TATG
NspV 2977 TTCGAA TT^CGAA
PflFI 189 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1069 CACGTG CAC^GTG
PmlI 1069 CACGTG CAC^GTG
PshAI 2803 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2036 GAGCTC GAGCT^C
SfuI 2977 TTCGAA TT^CGAA
SspI 2509 AATATT AAT^ATT
Tth111I 189 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1600 CCAGGCCTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2970 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1508 GTATAC GT^[AC][GT]AC
2559 GTAGAC
AccII 1694 CGCG CG^CG
3117 CGCG
AflII 796 CTTAAG C^TTAAG
3621 CTTAAG
ApaI 553 GGGCCC GGGCC^C
2460 GGGCCC
BalI 2106 TGGCCA TGG^CCA
2466 TGGCCA
BclI 1542 TGATCA T^GATCA
2602 TGATCA
BfrI 796 CTTAAG C^TTAAG
3621 CTTAAG
BglII 2007 AGATCT A^GATCT
2769 AGATCT
BsaAI 1069 CACGTG [CT]AC^GT[GA]
1684 CACGTA
BsaI 3435 GAGACC GGTCTC(1/5)
1839 GGTCTC
BsgI 1805 GTGCAG GTGCAG(16/14)
2178 GTGCAG
BspHI 175 TCATGA T^CATGA
1465 TCATGA
BsrBI 1125 GAGCGG CCGCTC(-3/-3)
1165 GAGCGG
BstAPI 2589 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3099 GCAGAGATTGC
BstUI 1694 CGCG CG^CG
3117 CGCG
CfoI 1977 GCGC GCG^C
3009 GCGC
DsaI 1079 CCGTGG C^C[GA][CT]GG
1265 CCACGG
EcoT22I 1281 ATGCAT ATGCA^T
3603 ATGCAT
FauI 1144 CCCGC CCCGC(4/6)
1101 GCGGG
FbaI 1542 TGATCA T^GATCA
2602 TGATCA
HapII 920 CCGG C^CGG
1946 CCGG
HhaI 1977 GCGC GCG^C
3009 GCGC
HinP1I 1977 GCGC G^CGC
3009 GCGC
HincII 2616 GTTGAC GT[CT]^[GA]AC
3492 GTTGAC
HindII 2616 GTTGAC GT[CT]^[GA]AC
3492 GTTGAC
HpaII 920 CCGG C^CGG
1946 CCGG
MluNI 2106 TGGCCA TGG^CCA
2466 TGGCCA
MscI 2106 TGGCCA TGG^CCA
2466 TGGCCA
MspI 920 CCGG C^CGG
1946 CCGG
MvnI 1694 CGCG CG^CG
3117 CGCG
NheI 669 GCTAGC G^CTAGC
3153 GCTAGC
NsiI 1281 ATGCAT ATGCA^T
3603 ATGCAT
PpuMI 2099 GGGTCCT [GA]G^G[AT]CC[CT]
2962 AGGTCCT
PstI 409 CTGCAG CTGCA^G
1844 CTGCAG
RcaI 175 TCATGA T^CATGA
1465 TCATGA
SphI 36 GCATGC GCATG^C
1279 GCATGC
StuI 1589 AGGCCT AGG^CCT
1602 AGGCCT
TaqI 2978 TCGA T^CGA
3176 TCGA
TthHB8I 2978 TCGA T^CGA
3176 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII