##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AgeI AhdI ApaI AscI
AseI AsnI AspEI AvaI AviII
AvrII BbeI BciVI BlnI BsiEI
BsiWI BsoBI BsrFI BssHII BstAPI
BstBI BstEII BstPI Cfr10I CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRV FseI FspI
HincII HindII HindIII HpaI KasI
KspI MfeI MunI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2860 GGTACC G^GTACC
AccI 5031 GTATAC GT^[AC][GT]AC
AccIII 4233 TCCGGA T^CCGGA
AcyI 3819 GACGCC G[GA]^CG[CT]C
Alw44I 1358 GTGCAC G^TGCAC
Aor51HI 3615 AGCGCT AGC^GCT
ApaLI 1358 GTGCAC G^TGCAC
Asp718I 2860 GGTACC G^GTACC
BalI 1984 TGGCCA TGG^CCA
BamHI 3194 GGATCC G^GATCC
BbrPI 3865 CACGTG CAC^GTG
BbvCI 4247 CCTCAGC CCTCAGC(-5/-2)
BcnI 4794 CCCGG CC^[GC]GG
BglI 2673 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4219 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4219 GCTCAGC GC^T[ACGT]AGC
BsaBI 2281 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3819 GACGCC G[GA]^CG[CT]C
BsaWI 4233 TCCGGA [AT]^CCGG[AT]
BseAI 4233 TCCGGA T^CCGGA
BsmBI 2657 CGTCTC CGTCTC(1/5)
BsmI 3310 GAATGC GAATGC(1/-1)
Bsp1407I 3084 TGTACA T^GTACA
BspDI 3268 ATCGAT AT^CGAT
BspEI 4233 TCCGGA T^CCGGA
BsrGI 3084 TGTACA T^GTACA
Bst1107I 5031 GTATAC GTA^TAC
BstXI 1512 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5031 GTATAC GTA^TAC
CelII 4219 GCTCAGC GC^T[ACGT]AGC
ClaI 3268 ATCGAT AT^CGAT
DrdI 5088 GACAAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3615 AGCGCT AGC^GCT
EcoRI 597 GAATTC G^AATTC
HaeII 3615 AGCGCT [GA]GCGC^[CT]
Hin1I 3819 GACGCC G[GA]^CG[CT]C
KpnI 2860 GGTACC GGTAC^C
MamI 2281 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1984 TGGCCA TGG^CCA
MroI 4233 TCCGGA T^CCGGA
MscI 1984 TGGCCA TGG^CCA
NciI 4794 CCCGG CC^[GC]GG
NheI 1481 GCTAGC G^CTAGC
PmaCI 3865 CACGTG CAC^GTG
PmlI 3865 CACGTG CAC^GTG
PpuMI 3065 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 249 CAGCTG CAG^CTG
SapI 1925 GAAGAGC GCTCTTC(1/4)
SpeI 818 ACTAGT A^CTAGT
SphI 1065 GCATGC GCATG^C
SspBI 3084 TGTACA T^GTACA
XbaI 2076 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 578 CTTAAG C^TTAAG
2623 CTTAAG
AspI 3658 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5021 GACCATGTC
BanII 3511 GGGCTC G[GA]GC[CT]^C
4729 GAGCCC
BclI 1224 TGATCA T^GATCA
2811 TGATCA
BfrI 578 CTTAAG C^TTAAG
2623 CTTAAG
BsaAI 2756 CACGTA [CT]AC^GT[GA]
3865 CACGTG
BspLU11I 4818 ACATGT A^CATGT
5002 ACATGT
BsrBI 1928 GAGCGG CCGCTC(-3/-3)
4166 GAGCGG
BsrDI 3699 CATTGC GCAATG(2/0)
4812 CATTGC
BssSI 1108 CACGAG CACGAG(-5/-1)
4657 CTCGTG
CfoI 1312 GCGC GCG^C
3616 GCGC
EaeI 1984 TGGCCA [CT]^GGCC[GA]
4698 CGGCCA
EcoT22I 346 ATGCAT ATGCA^T
4900 ATGCAT
FbaI 1224 TGATCA T^GATCA
2811 TGATCA
HhaI 1312 GCGC GCG^C
3616 GCGC
HinP1I 1312 GCGC G^CGC
3616 GCGC
MluI 1389 ACGCGT A^CGCGT
3939 ACGCGT
MslI 1361 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4546 CATGAAGATG
MspA1I 249 CAGCTG C[AC]G^C[GT]G
1197 CCGCTG
NcoI 1014 CCATGG C^CATGG
4096 CCATGG
NsiI 346 ATGCAT ATGCA^T
4900 ATGCAT
PflFI 3658 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5021 GACCATGTC
Tth111I 3658 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5021 GACCATGTC
XcmI 1853 CCAAAAATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3181 CCACTGCACACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaII BanI
BbsI BbvI BcgI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI Psp1406I PstI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI