##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AscI Asp718I AviII BbeI
BbrPI BbvCI BcgI BclI BfrI
BsaAI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrFI BssHII BstBI
BstEII BstPI BstUI Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2306 GACGTC GACGT^C
AcsI 2994 GAATTC [GA]^AATT[CT]
Alw44I 3341 GTGCAC G^TGCAC
Aor51HI 2434 AGCGCT AGC^GCT
ApaI 5203 GGGCCC GGGCC^C
ApaLI 3341 GTGCAC G^TGCAC
ApoI 2994 GAATTC [GA]^AATT[CT]
AseI 4181 ATTAAT AT^TAAT
AsnI 4181 ATTAAT AT^TAAT
AvrII 1480 CCTAGG C^CTAGG
BciVI 1984 GTATCC GTATCC(6/5)
BcnI 5423 CCGGG CC^[GC]GG
BglI 4908 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1154 AGATCT A^GATCT
BlnI 1480 CCTAGG C^CTAGG
BsaBI 4360 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 266 TGTACA T^GTACA
BsrGI 266 TGTACA T^GTACA
BssSI 4492 CTCGTG CACGAG(-5/-1)
Eco47III 2434 AGCGCT AGC^GCT
EcoRI 2994 GAATTC G^AATTC
HincII 5506 GTTAAC GT[CT]^[GA]AC
HindII 5506 GTTAAC GT[CT]^[GA]AC
HindIII 2415 AAGCTT A^AGCTT
HpaI 5506 GTTAAC GTT^AAC
MamI 4360 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 5423 CCGGG CC^[GC]GG
NdeI 5535 CATATG CA^TATG
NheI 302 GCTAGC G^CTAGC
PaeR7I 2430 CTCGAG C^TCGAG
PshAI 3643 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4181 ATTAAT AT^TAAT
SbfI 4686 CCTGCAGG CCTGCA^GG
SphI 2173 GCATGC GCATG^C
Sse8387I 4686 CCTGCAGG CCTGCA^GG
SspBI 266 TGTACA T^GTACA
XcmI 3834 CCAGCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2430 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 3719 ACATGT A^C[GA][CT]GT
4106 ACATGT
AhdI 568 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5476 GACCTGCTGTC
AspEI 568 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5476 GACCTGCTGTC
AspI 3685 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5176 GACACTGTC
BamHI 1086 GGATCC G^GATCC
3113 GGATCC
BpmI 2311 CTCCAG CTGGAG(16/14)
4962 CTGGAG
BsmI 3514 GAATGC GAATGC(1/-1)
4451 GCATTC
BspHI 1767 TCATGA T^CATGA
5378 TCATGA
BspLU11I 3719 ACATGT A^CATGT
4106 ACATGT
BsrDI 3087 GCAATG GCAATG(2/0)
4355 GCAATG
Eam1105I 568 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5476 GACCTGCTGTC
EarI 1713 CTCTTC CTCTTC(1/4)
3074 CTCTTC
EcoNI 1077 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2340 CCTCAGGAAGG
HapII 4527 CCGG C^CGG
5423 CCGG
HpaII 4527 CCGG C^CGG
5423 CCGG
Ksp632I 1713 CTCTTC CTCTTC(1/4)
3074 CTCTTC
MspI 4527 CCGG C^CGG
5423 CCGG
NcoI 3118 CCATGG C^CATGG
3679 CCATGG
PflFI 3685 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5176 GACACTGTC
RcaI 1767 TCATGA T^CATGA
5378 TCATGA
ScaI 1702 AGTACT AGT^ACT
2957 AGTACT
SexAI 2374 ACCTGGT A^CC[AT]GGT
5383 ACCAGGT
Tth111I 3685 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5176 GACACTGTC
XbaI 4162 TCTAGA T^CTAGA
5191 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AocI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BfaI BlpI BmyI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI Bst1107I
BstAPI BstF5I BstNI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI