################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AscI Asp718I AviII BbeI BbrPI BbvCI BcgI BclI BfrI BsaAI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 2306 GACGTC GACGT^C AcsI 2994 GAATTC [GA]^AATT[CT] Alw44I 3341 GTGCAC G^TGCAC Aor51HI 2434 AGCGCT AGC^GCT ApaI 5203 GGGCCC GGGCC^C ApaLI 3341 GTGCAC G^TGCAC ApoI 2994 GAATTC [GA]^AATT[CT] AseI 4181 ATTAAT AT^TAAT AsnI 4181 ATTAAT AT^TAAT AvrII 1480 CCTAGG C^CTAGG BciVI 1984 GTATCC GTATCC(6/5) BcnI 5423 CCGGG CC^[GC]GG BglI 4908 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1154 AGATCT A^GATCT BlnI 1480 CCTAGG C^CTAGG BsaBI 4360 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 266 TGTACA T^GTACA BsrGI 266 TGTACA T^GTACA BssSI 4492 CTCGTG CACGAG(-5/-1) Eco47III 2434 AGCGCT AGC^GCT EcoRI 2994 GAATTC G^AATTC HincII 5506 GTTAAC GT[CT]^[GA]AC HindII 5506 GTTAAC GT[CT]^[GA]AC HindIII 2415 AAGCTT A^AGCTT HpaI 5506 GTTAAC GTT^AAC MamI 4360 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 5423 CCGGG CC^[GC]GG NdeI 5535 CATATG CA^TATG NheI 302 GCTAGC G^CTAGC PaeR7I 2430 CTCGAG C^TCGAG PshAI 3643 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4181 ATTAAT AT^TAAT SbfI 4686 CCTGCAGG CCTGCA^GG SphI 2173 GCATGC GCATG^C Sse8387I 4686 CCTGCAGG CCTGCA^GG SspBI 266 TGTACA T^GTACA XcmI 3834 CCAGCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2430 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 3719 ACATGT A^C[GA][CT]GT 4106 ACATGT AhdI 568 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5476 GACCTGCTGTC AspEI 568 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5476 GACCTGCTGTC AspI 3685 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5176 GACACTGTC BamHI 1086 GGATCC G^GATCC 3113 GGATCC BpmI 2311 CTCCAG CTGGAG(16/14) 4962 CTGGAG BsmI 3514 GAATGC GAATGC(1/-1) 4451 GCATTC BspHI 1767 TCATGA T^CATGA 5378 TCATGA BspLU11I 3719 ACATGT A^CATGT 4106 ACATGT BsrDI 3087 GCAATG GCAATG(2/0) 4355 GCAATG Eam1105I 568 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5476 GACCTGCTGTC EarI 1713 CTCTTC CTCTTC(1/4) 3074 CTCTTC EcoNI 1077 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2340 CCTCAGGAAGG HapII 4527 CCGG C^CGG 5423 CCGG HpaII 4527 CCGG C^CGG 5423 CCGG Ksp632I 1713 CTCTTC CTCTTC(1/4) 3074 CTCTTC MspI 4527 CCGG C^CGG 5423 CCGG NcoI 3118 CCATGG C^CATGG 3679 CCATGG PflFI 3685 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5176 GACACTGTC RcaI 1767 TCATGA T^CATGA 5378 TCATGA ScaI 1702 AGTACT AGT^ACT 2957 AGTACT SexAI 2374 ACCTGGT A^CC[AT]GGT 5383 ACCAGGT Tth111I 3685 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5176 GACACTGTC XbaI 4162 TCTAGA T^CTAGA 5191 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AlwNI AocI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BfaI BlpI BmyI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI Bst1107I BstAPI BstF5I BstNI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HgaI HhaI Hin1I HinP1I HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI