##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
ApaLI AscI AviII BcgI BciVI
BclI BsaBI BsiWI BspDI BsrDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI SbfI SfiI
SfuI SgrAI SpeI SphI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 4052 TCCGGA T^CCGGA
AflII 3373 CTTAAG C^TTAAG
AflIII 4823 ACATGT A^C[GA][CT]GT
AhdI 134 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4495 ATTAAT AT^TAAT
AsnI 4495 ATTAAT AT^TAAT
AspEI 134 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3688 CCTAGG C^CTAGG
BamHI 3133 GGATCC G^GATCC
BbrPI 3822 CACGTG CAC^GTG
BfrI 3373 CTTAAG C^TTAAG
BlnI 3688 CCTAGG C^CTAGG
BsaI 1221 GAGACC GGTCTC(1/5)
BseAI 4052 TCCGGA T^CCGGA
BsiEI 2097 CGGCCG CG[GA][CT]^CG
BspEI 4052 TCCGGA T^CCGGA
BspHI 3952 TCATGA T^CATGA
BspLU11I 4823 ACATGT A^CATGT
BssSI 4572 CACGAG CACGAG(-5/-1)
BstAPI 2612 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 4456 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2097 CGGCCG C^GGCCG
Eam1105I 134 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2097 CGGCCG C^GGCCG
Eco52I 2097 CGGCCG C^GGCCG
EcoRI 353 GAATTC G^AATTC
KspI 2094 CCGCGG CCGC^GG
MroI 4052 TCCGGA T^CCGGA
NheI 4451 GCTAGC G^CTAGC
NspI 4823 ACATGT [GA]CATG^[CT]
PflMI 2953 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3822 CACGTG CAC^GTG
PmlI 3822 CACGTG CAC^GTG
PshBI 4495 ATTAAT AT^TAAT
RcaI 3952 TCATGA T^CATGA
SacI 2089 GAGCTC GAGCT^C
SacII 2094 CCGCGG CCGC^GG
ScaI 531 AGTACT AGT^ACT
SexAI 717 ACCAGGT A^CC[AT]GGT
SnaBI 1850 TACGTA TAC^GTA
SspI 4946 AATATT AAT^ATT
Van91I 2953 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 600 GGTACC G^GTACC
2738 GGTACC
AlwNI 1676 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2613 CAGCTGCTG
AocI 774 CCTCAGG CC^T[ACGT]AGG
1762 CCTCAGG
Aor51HI 672 AGCGCT AGC^GCT
3925 AGCGCT
ApaI 3831 GGGCCC GGGCC^C
4643 GGGCCC
Asp718I 600 GGTACC G^GTACC
2738 GGTACC
AspHI 1097 GAGCAC G[AT]GC[AT]^C
2089 GAGCTC
AspI 1550 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4718 GACCCTGTC
BbeI 1793 GGCGCC GGCGC^C
4306 GGCGCC
BbvCI 591 CCTCAGC CCTCAGC(-5/-2)
648 CCTCAGC
BglI 3914 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4309 GCCTGCGCGGC
BglII 624 AGATCT A^GATCT
3078 AGATCT
BsaWI 55 ACCGGA [AT]^CCGG[AT]
4052 TCCGGA
BsiHKAI 1097 GAGCAC G[AT]GC[AT]^C
2089 GAGCTC
BsmBI 4381 CGTCTC CGTCTC(1/5)
2600 GAGACG
BsmI 1156 GAATGC GAATGC(1/-1)
2205 GAATGC
BstEII 523 GGTGACC G^GT[ACGT]ACC
3622 GGTTACC
BstPI 523 GGTGACC G^GT[ACGT]ACC
3622 GGTTACC
Bsu36I 774 CCTCAGG CC^T[ACGT]AGG
1762 CCTCAGG
Eco47III 672 AGCGCT AGC^GCT
3925 AGCGCT
Eco81I 774 CCTCAGG CC^T[ACGT]AGG
1762 CCTCAGG
EcoO65I 523 GGTGACC G^GT[ACGT]ACC
3622 GGTTACC
FauI 3166 CCCGC CCCGC(4/6)
3365 CCCGC
KasI 1793 GGCGCC G^GCGCC
4306 GGCGCC
KpnI 600 GGTACC GGTAC^C
2738 GGTACC
NarI 1793 GGCGCC GG^CGCC
4306 GGCGCC
PflFI 1550 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4718 GACCCTGTC
SapI 1903 GAAGAGC GCTCTTC(1/4)
4400 GCTCTTC
SfoI 1793 GGCGCC GGC^GCC
4306 GGCGCC
SmaI 4646 CCCGGG CCC^GGG
4752 CCCGGG
TfiI 1985 GATTC G^A[AT]TC
2873 GAATC
Tth111I 1550 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4718 GACCCTGTC
XmaCI 4646 CCCGGG C^CCGGG
4752 CCCGGG
XmaI 4646 CCCGGG C^CCGGG
4752 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PaeR7I
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI XhoII XmnI