################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII AccI AclI AgeI Alw44I ApaLI AscI AviII BcgI BciVI BclI BsaBI BsiWI BspDI BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshAI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SgrAI SpeI SphI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 4052 TCCGGA T^CCGGA AflII 3373 CTTAAG C^TTAAG AflIII 4823 ACATGT A^C[GA][CT]GT AhdI 134 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 4495 ATTAAT AT^TAAT AsnI 4495 ATTAAT AT^TAAT AspEI 134 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3688 CCTAGG C^CTAGG BamHI 3133 GGATCC G^GATCC BbrPI 3822 CACGTG CAC^GTG BfrI 3373 CTTAAG C^TTAAG BlnI 3688 CCTAGG C^CTAGG BsaI 1221 GAGACC GGTCTC(1/5) BseAI 4052 TCCGGA T^CCGGA BsiEI 2097 CGGCCG CG[GA][CT]^CG BspEI 4052 TCCGGA T^CCGGA BspHI 3952 TCATGA T^CATGA BspLU11I 4823 ACATGT A^CATGT BssSI 4572 CACGAG CACGAG(-5/-1) BstAPI 2612 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 4456 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2097 CGGCCG C^GGCCG Eam1105I 134 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2097 CGGCCG C^GGCCG Eco52I 2097 CGGCCG C^GGCCG EcoRI 353 GAATTC G^AATTC KspI 2094 CCGCGG CCGC^GG MroI 4052 TCCGGA T^CCGGA NheI 4451 GCTAGC G^CTAGC NspI 4823 ACATGT [GA]CATG^[CT] PflMI 2953 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3822 CACGTG CAC^GTG PmlI 3822 CACGTG CAC^GTG PshBI 4495 ATTAAT AT^TAAT RcaI 3952 TCATGA T^CATGA SacI 2089 GAGCTC GAGCT^C SacII 2094 CCGCGG CCGC^GG ScaI 531 AGTACT AGT^ACT SexAI 717 ACCAGGT A^CC[AT]GGT SnaBI 1850 TACGTA TAC^GTA SspI 4946 AATATT AAT^ATT Van91I 2953 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 600 GGTACC G^GTACC 2738 GGTACC AlwNI 1676 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 2613 CAGCTGCTG AocI 774 CCTCAGG CC^T[ACGT]AGG 1762 CCTCAGG Aor51HI 672 AGCGCT AGC^GCT 3925 AGCGCT ApaI 3831 GGGCCC GGGCC^C 4643 GGGCCC Asp718I 600 GGTACC G^GTACC 2738 GGTACC AspHI 1097 GAGCAC G[AT]GC[AT]^C 2089 GAGCTC AspI 1550 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4718 GACCCTGTC BbeI 1793 GGCGCC GGCGC^C 4306 GGCGCC BbvCI 591 CCTCAGC CCTCAGC(-5/-2) 648 CCTCAGC BglI 3914 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4309 GCCTGCGCGGC BglII 624 AGATCT A^GATCT 3078 AGATCT BsaWI 55 ACCGGA [AT]^CCGG[AT] 4052 TCCGGA BsiHKAI 1097 GAGCAC G[AT]GC[AT]^C 2089 GAGCTC BsmBI 4381 CGTCTC CGTCTC(1/5) 2600 GAGACG BsmI 1156 GAATGC GAATGC(1/-1) 2205 GAATGC BstEII 523 GGTGACC G^GT[ACGT]ACC 3622 GGTTACC BstPI 523 GGTGACC G^GT[ACGT]ACC 3622 GGTTACC Bsu36I 774 CCTCAGG CC^T[ACGT]AGG 1762 CCTCAGG Eco47III 672 AGCGCT AGC^GCT 3925 AGCGCT Eco81I 774 CCTCAGG CC^T[ACGT]AGG 1762 CCTCAGG EcoO65I 523 GGTGACC G^GT[ACGT]ACC 3622 GGTTACC FauI 3166 CCCGC CCCGC(4/6) 3365 CCCGC KasI 1793 GGCGCC G^GCGCC 4306 GGCGCC KpnI 600 GGTACC GGTAC^C 2738 GGTACC NarI 1793 GGCGCC GG^CGCC 4306 GGCGCC PflFI 1550 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4718 GACCCTGTC SapI 1903 GAAGAGC GCTCTTC(1/4) 4400 GCTCTTC SfoI 1793 GGCGCC GGC^GCC 4306 GGCGCC SmaI 4646 CCCGGG CCC^GGG 4752 CCCGGG TfiI 1985 GATTC G^A[AT]TC 2873 GAATC Tth111I 1550 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4718 GACCCTGTC XmaCI 4646 CCCGGG C^CCGGG 4752 CCCGGG XmaI 4646 CCCGGG C^CCGGG 4752 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PaeR7I PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII XmnI