##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
AscI Asp718I AspEI AvaI AviII
BamHI BanI BbeI BbrPI BbvCI
BfrI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspEI BsrBI BsrGI BssHII BstAPI
BstUI Bsu36I CelII CpoI DrdI
Eam1105I Eco81I FauI FseI FspI
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI MvnI NarI
NcoI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacII
SalI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SphI SspBI
StuI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Alw44I 1450 GTGCAC G^TGCAC
AlwNI 4054 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 4659 AGCGCT AGC^GCT
ApaI 2920 GGGCCC GGGCC^C
ApaLI 1450 GTGCAC G^TGCAC
AseI 2327 ATTAAT AT^TAAT
AsnI 2327 ATTAAT AT^TAAT
Asp700I 3061 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1301 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4882 CCTAGG C^CTAGG
BcgI 2444 GCAGAAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1034 CCGGG CC^[GC]GG
BglI 2769 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4882 CCTAGG C^CTAGG
BpmI 2100 CTGGAG CTGGAG(16/14)
BsaBI 1512 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 546 GGTCTC GGTCTC(1/5)
BsgI 2341 GTGCAG GTGCAG(16/14)
BsiEI 1318 CGGCCG CG[GA][CT]^CG
BspMI 1582 ACCTGC ACCTGC(4/8)
BsrFI 3749 GCCGGC [GA]^CCGG[CT]
BssSI 2541 CACGAG CACGAG(-5/-1)
Bst1107I 3118 GTATAC GTA^TAC
BstBI 2580 TTCGAA TT^CGAA
BstEII 3705 GGTCACC G^GT[ACGT]ACC
BstPI 3705 GGTCACC G^GT[ACGT]ACC
BstXI 1707 CCACATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3118 GTATAC GTA^TAC
Cfr10I 3749 GCCGGC [GA]^CCGG[CT]
DraIII 2986 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1318 CGGCCG C^GGCCG
EclXI 1318 CGGCCG C^GGCCG
Eco47III 4659 AGCGCT AGC^GCT
Eco52I 1318 CGGCCG C^GGCCG
EcoNI 4264 CCTATTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3705 GGTCACC G^GT[ACGT]ACC
HaeII 4659 AGCGCT [GA]GCGC^[CT]
HgaI 1305 GCGTC GACGC(5/10)
HpaI 963 GTTAAC GTT^AAC
MamI 1512 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 4057 CCGCTG C[AC]G^C[GT]G
NaeI 3749 GCCGGC GCC^GGC
NciI 1034 CCGGG CC^[GC]GG
NdeI 4911 CATATG CA^TATG
NgoMIV 3749 GCCGGC G^CCGGC
NheI 1221 GCTAGC G^CTAGC
NspV 2580 TTCGAA TT^CGAA
PflFI 1301 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2394 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2327 ATTAAT AT^TAAT
SacI 3555 GAGCTC GAGCT^C
SapI 2313 GAAGAGC GCTCTTC(1/4)
SbfI 332 CCTGCAGG CCTGCA^GG
SfuI 2580 TTCGAA TT^CGAA
SnaBI 3121 TACGTA TAC^GTA
SpeI 2012 ACTAGT A^CTAGT
Sse8387I 332 CCTGCAGG CCTGCA^GG
SwaI 3257 ATTTAAAT ATTT^AAAT
Tth111I 1301 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2394 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3061 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 4057 CCGC CCGC(-3/-1)
1317 GCGG
BanII 2920 GGGCCC G[GA]GC[CT]^C
3555 GAGCTC
BciVI 1030 GTATCC GTATCC(6/5)
2244 GTATCC
BspDI 3394 ATCGAT AT^CGAT
4545 ATCGAT
BspHI 2122 TCATGA T^CATGA
2833 TCATGA
BsrDI 835 GCAATG GCAATG(2/0)
1669 GCAATG
CfoI 4156 GCGC GCG^C
4660 GCGC
ClaI 3394 ATCGAT AT^CGAT
4545 ATCGAT
DsaI 375 CCACGG C^C[GA][CT]GG
1041 CCACGG
EcoT22I 2421 ATGCAT ATGCA^T
3409 ATGCAT
HapII 1034 CCGG C^CGG
3750 CCGG
HhaI 4156 GCGC GCG^C
4660 GCGC
HinP1I 4156 GCGC G^CGC
4660 GCGC
HpaII 1034 CCGG C^CGG
3750 CCGG
MspI 1034 CCGG C^CGG
3750 CCGG
NsiI 2421 ATGCAT ATGCA^T
3409 ATGCAT
RcaI 2122 TCATGA T^CATGA
2833 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BalI
BbsI BbvI BclI BfaI BglII
BmyI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoRV
EcoT14I FbaI Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII