##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AclI AgeI Aor51HI
AscI AseI AsnI AspI BcgI
BclI BglI BglII BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EarI
Eco47III EcoRV FbaI FseI Ksp632I
KspI MamI MfeI MluI MunI
NdeI NotI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3546 GGTACC G^GTACC
AccIII 2460 TCCGGA T^CCGGA
AflII 273 CTTAAG C^TTAAG
AflIII 1815 ACGTGT A^C[GA][CT]GT
AhdI 4202 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2609 GTGCAC G^TGCAC
ApaLI 2609 GTGCAC G^TGCAC
Asp700I 1504 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3546 GGTACC G^GTACC
AspEI 4202 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2256 CCTAGG C^CTAGG
BamHI 2785 GGATCC G^GATCC
BfrI 273 CTTAAG C^TTAAG
BlnI 2256 CCTAGG C^CTAGG
BsaWI 2460 TCCGGA [AT]^CCGG[AT]
BseAI 2460 TCCGGA T^CCGGA
BspEI 2460 TCCGGA T^CCGGA
BsrBI 192 CCGCTC CCGCTC(-3/-3)
BstAPI 380 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3937 GGTGACC G^GT[ACGT]ACC
BstPI 3937 GGTGACC G^GT[ACGT]ACC
DraI 4593 TTTAAA TTT^AAA
DraIII 645 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3726 CGGCCG C^GGCCG
Eam1105I 4202 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3726 CGGCCG C^GGCCG
Eco52I 3726 CGGCCG C^GGCCG
EcoO65I 3937 GGTGACC G^GT[ACGT]ACC
EcoT22I 793 ATGCAT ATGCA^T
HindIII 4001 AAGCTT A^AGCTT
KpnI 3546 GGTACC GGTAC^C
MroI 2460 TCCGGA T^CCGGA
NruI 398 TCGCGA TCG^CGA
NsiI 793 ATGCAT ATGCA^T
PaeR7I 3061 CTCGAG C^TCGAG
PflMI 2299 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 2811 CGATCG CGAT^CG
SacI 3627 GAGCTC GAGCT^C
ScaI 1327 AGTACT AGT^ACT
Van91I 2299 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3061 CTCGAG C^TCGAG
XmnI 1504 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AviII 641 TGCGCA TGC^GCA
2314 TGCGCA
BbeI 3875 GGCGCC GGCGC^C
4351 GGCGCC
BbvCI 3140 CCTCAGC CCTCAGC(-5/-2)
4451 GCTGAGG
BciVI 4140 GGATAC GTATCC(6/5)
4156 GTATCC
EcoRI 1041 GAATTC G^AATTC
4118 GAATTC
FspI 641 TGCGCA TGC^GCA
2314 TGCGCA
HincII 1811 GTTAAC GT[CT]^[GA]AC
3077 GTTAAC
HindII 1811 GTTAAC GT[CT]^[GA]AC
3077 GTTAAC
HpaI 1811 GTTAAC GTT^AAC
3077 GTTAAC
KasI 3875 GGCGCC G^GCGCC
4351 GGCGCC
NaeI 2661 GCCGGC GCC^GGC
4494 GCCGGC
NarI 3875 GGCGCC GG^CGCC
4351 GGCGCC
NgoMIV 2661 GCCGGC G^CCGGC
4494 GCCGGC
PshAI 2705 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3786 GACCTGAGTC
SbfI 812 CCTGCAGG CCTGCA^GG
2567 CCTGCAGG
SfoI 3875 GGCGCC GGC^GCC
4351 GGCGCC
Sse8387I 812 CCTGCAGG CCTGCA^GG
2567 CCTGCAGG
StuI 622 AGGCCT AGG^CCT
2908 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII