################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccII AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BlnI BsiEI BsiWI Bsp1407I BspDI BsrBI BsrGI BssHII BstBI BstUI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HincII HindII HpaI KpnI KspI MfeI MluI MunI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 4962 TCCGGA T^CCGGA AcyI 4335 GGCGCC G[GA]^CG[CT]C AflII 5264 CTTAAG C^TTAAG AlwNI 366 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 2140 GGATCC G^GATCC BbeI 4335 GGCGCC GGCGC^C BfrI 5264 CTTAAG C^TTAAG BglII 1208 AGATCT A^GATCT BsaBI 1533 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4335 GGCGCC G[GA]^CG[CT]C BseAI 4962 TCCGGA T^CCGGA BsmBI 4011 CGTCTC CGTCTC(1/5) BspEI 4962 TCCGGA T^CCGGA BspHI 593 TCATGA T^CATGA Bst1107I 1433 GTATAC GTA^TAC BstAPI 4980 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 5324 GGTGACC G^GT[ACGT]ACC BstPI 5324 GGTGACC G^GT[ACGT]ACC BstZ17I 1433 GTATAC GTA^TAC DraI 1470 TTTAAA TTT^AAA DraIII 4500 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 5324 GGTGACC G^GT[ACGT]ACC EcoRI 697 GAATTC G^AATTC Hin1I 4335 GGCGCC G[GA]^CG[CT]C HindIII 5261 AAGCTT A^AGCTT KasI 4335 GGCGCC G^GCGCC MamI 1533 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 4962 TCCGGA T^CCGGA NarI 4335 GGCGCC GG^CGCC NdeI 3361 CATATG CA^TATG PflMI 3568 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4564 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 593 TCATGA T^CATGA SapI 3183 GCTCTTC GCTCTTC(1/4) SbfI 371 CCTGCAGG CCTGCA^GG SexAI 2047 ACCAGGT A^CC[AT]GGT SfiI 1748 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 4335 GGCGCC GGC^GCC SmaI 4763 CCCGGG CCC^GGG Sse8387I 371 CCTGCAGG CCTGCA^GG TaqI 3459 TCGA T^CGA TfiI 941 GATTC G^A[AT]TC TthHB8I 3459 TCGA T^CGA Van91I 3568 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 4763 CCCGGG C^CCGGG XmaI 4763 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 1433 GTATAC GT^[AC][GT]AC 2788 GTCTAC ApaI 2214 GGGCCC GGGCC^C 4477 GGGCCC Asp700I 1875 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4749 GAAGCCATTC BcgI 4466 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5037 GCAGTGACCTCG BclI 2699 TGATCA T^GATCA 4361 TGATCA BsaWI 2728 TCCGGT [AT]^CCGG[AT] 4962 TCCGGA BsmI 786 GCATTC GAATGC(1/-1) 3895 GCATTC BspLU11I 891 ACATGT A^CATGT 922 ACATGT BsrDI 4274 CATTGC GCAATG(2/0) 4779 GCAATG FbaI 2699 TGATCA T^GATCA 4361 TGATCA NcoI 3593 CCATGG C^CATGG 4481 CCATGG PpuMI 651 AGGACCC [GA]G^G[AT]CC[CT] 4814 AGGTCCT ScaI 301 AGTACT AGT^ACT 3912 AGTACT XcmI 3864 CCAGTCCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4671 CCATGAAGACTGTGG XmnI 1875 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4749 GAAGCCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AocI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PmaCI PmlI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII