##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BlnI BsiEI BsiWI Bsp1407I BspDI
BsrBI BsrGI BssHII BstBI BstUI
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HincII HindII HpaI KpnI KspI
MfeI MluI MunI MvnI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 4962 TCCGGA T^CCGGA
AcyI 4335 GGCGCC G[GA]^CG[CT]C
AflII 5264 CTTAAG C^TTAAG
AlwNI 366 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 2140 GGATCC G^GATCC
BbeI 4335 GGCGCC GGCGC^C
BfrI 5264 CTTAAG C^TTAAG
BglII 1208 AGATCT A^GATCT
BsaBI 1533 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4335 GGCGCC G[GA]^CG[CT]C
BseAI 4962 TCCGGA T^CCGGA
BsmBI 4011 CGTCTC CGTCTC(1/5)
BspEI 4962 TCCGGA T^CCGGA
BspHI 593 TCATGA T^CATGA
Bst1107I 1433 GTATAC GTA^TAC
BstAPI 4980 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5324 GGTGACC G^GT[ACGT]ACC
BstPI 5324 GGTGACC G^GT[ACGT]ACC
BstZ17I 1433 GTATAC GTA^TAC
DraI 1470 TTTAAA TTT^AAA
DraIII 4500 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 5324 GGTGACC G^GT[ACGT]ACC
EcoRI 697 GAATTC G^AATTC
Hin1I 4335 GGCGCC G[GA]^CG[CT]C
HindIII 5261 AAGCTT A^AGCTT
KasI 4335 GGCGCC G^GCGCC
MamI 1533 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4962 TCCGGA T^CCGGA
NarI 4335 GGCGCC GG^CGCC
NdeI 3361 CATATG CA^TATG
PflMI 3568 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4564 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 593 TCATGA T^CATGA
SapI 3183 GCTCTTC GCTCTTC(1/4)
SbfI 371 CCTGCAGG CCTGCA^GG
SexAI 2047 ACCAGGT A^CC[AT]GGT
SfiI 1748 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4335 GGCGCC GGC^GCC
SmaI 4763 CCCGGG CCC^GGG
Sse8387I 371 CCTGCAGG CCTGCA^GG
TaqI 3459 TCGA T^CGA
TfiI 941 GATTC G^A[AT]TC
TthHB8I 3459 TCGA T^CGA
Van91I 3568 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 4763 CCCGGG C^CCGGG
XmaI 4763 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 1433 GTATAC GT^[AC][GT]AC
2788 GTCTAC
ApaI 2214 GGGCCC GGGCC^C
4477 GGGCCC
Asp700I 1875 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4749 GAAGCCATTC
BcgI 4466 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5037 GCAGTGACCTCG
BclI 2699 TGATCA T^GATCA
4361 TGATCA
BsaWI 2728 TCCGGT [AT]^CCGG[AT]
4962 TCCGGA
BsmI 786 GCATTC GAATGC(1/-1)
3895 GCATTC
BspLU11I 891 ACATGT A^CATGT
922 ACATGT
BsrDI 4274 CATTGC GCAATG(2/0)
4779 GCAATG
FbaI 2699 TGATCA T^GATCA
4361 TGATCA
NcoI 3593 CCATGG C^CATGG
4481 CCATGG
PpuMI 651 AGGACCC [GA]G^G[AT]CC[CT]
4814 AGGTCCT
ScaI 301 AGTACT AGT^ACT
3912 AGTACT
XcmI 3864 CCAGTCCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4671 CCATGAAGACTGTGG
XmnI 1875 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4749 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AocI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PmaCI PmlI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII