##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BlnI        BsiEI       BsiWI       Bsp1407I    BspDI       
BsrBI       BsrGI       BssHII      BstBI       BstUI       
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
HincII      HindII      HpaI        KpnI        KspI        
MfeI        MluI        MunI        MvnI        NaeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII       4962  TCCGGA               T^CCGGA
AcyI         4335  GGCGCC               G[GA]^CG[CT]C
AflII        5264  CTTAAG               C^TTAAG
AlwNI         366  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
BamHI        2140  GGATCC               G^GATCC
BbeI         4335  GGCGCC               GGCGC^C
BfrI         5264  CTTAAG               C^TTAAG
BglII        1208  AGATCT               A^GATCT
BsaBI        1533  GATAAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        4335  GGCGCC               G[GA]^CG[CT]C
BseAI        4962  TCCGGA               T^CCGGA
BsmBI        4011  CGTCTC               CGTCTC(1/5)
BspEI        4962  TCCGGA               T^CCGGA
BspHI         593  TCATGA               T^CATGA
Bst1107I     1433  GTATAC               GTA^TAC
BstAPI       4980  GCAGAATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       5324  GGTGACC              G^GT[ACGT]ACC
BstPI        5324  GGTGACC              G^GT[ACGT]ACC
BstZ17I      1433  GTATAC               GTA^TAC
DraI         1470  TTTAAA               TTT^AAA
DraIII       4500  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      5324  GGTGACC              G^GT[ACGT]ACC
EcoRI         697  GAATTC               G^AATTC
Hin1I        4335  GGCGCC               G[GA]^CG[CT]C
HindIII      5261  AAGCTT               A^AGCTT
KasI         4335  GGCGCC               G^GCGCC
MamI         1533  GATAAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         4962  TCCGGA               T^CCGGA
NarI         4335  GGCGCC               GG^CGCC
NdeI         3361  CATATG               CA^TATG
PflMI        3568  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        4564  GACGGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          593  TCATGA               T^CATGA
SapI         3183  GCTCTTC              GCTCTTC(1/4)
SbfI          371  CCTGCAGG             CCTGCA^GG
SexAI        2047  ACCAGGT              A^CC[AT]GGT
SfiI         1748  GGCCAGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         4335  GGCGCC               GGC^GCC
SmaI         4763  CCCGGG               CCC^GGG
Sse8387I      371  CCTGCAGG             CCTGCA^GG
TaqI         3459  TCGA                 T^CGA
TfiI          941  GATTC                G^A[AT]TC
TthHB8I      3459  TCGA                 T^CGA
Van91I       3568  CCAGCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        4763  CCCGGG               C^CCGGG
XmaI         4763  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI         1433  GTATAC               GT^[AC][GT]AC
             2788  GTCTAC              
ApaI         2214  GGGCCC               GGGCC^C
             4477  GGGCCC              
Asp700I      1875  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4749  GAAGCCATTC          
BcgI         4466  GCAAGGCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5037  GCAGTGACCTCG        
BclI         2699  TGATCA               T^GATCA
             4361  TGATCA              
BsaWI        2728  TCCGGT               [AT]^CCGG[AT]
             4962  TCCGGA              
BsmI          786  GCATTC               GAATGC(1/-1)
             3895  GCATTC              
BspLU11I      891  ACATGT               A^CATGT
              922  ACATGT              
BsrDI        4274  CATTGC               GCAATG(2/0)
             4779  GCAATG              
FbaI         2699  TGATCA               T^GATCA
             4361  TGATCA              
NcoI         3593  CCATGG               C^CATGG
             4481  CCATGG              
PpuMI         651  AGGACCC              [GA]G^G[AT]CC[CT]
             4814  AGGTCCT             
ScaI          301  AGTACT               AGT^ACT
             3912  AGTACT              
XcmI         3864  CCAGTCCCAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4671  CCATGAAGACTGTGG     
XmnI         1875  GAACTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4749  GAAGCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AocI        ApaLI       ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbsI        BbvCI       BbvI        
BciVI       BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PleI        
PmaCI       PmlI        PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StuI        StyI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII