##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BciVI BclI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HaeII KasI KpnI MluI MroI
NarI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SacI
SalI SapI SbfI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
StuI Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 256 CGCG CG^CG
AflII 2401 CTTAAG C^TTAAG
AflIII 1032 ACATGT A^C[GA][CT]GT
AseI 5019 ATTAAT AT^TAAT
AsnI 5019 ATTAAT AT^TAAT
AspHI 1564 GAGCAC G[AT]GC[AT]^C
BamHI 1149 GGATCC G^GATCC
BanI 1809 GGCACC G^G[CT][GA]CC
BbvCI 1501 GCTGAGG CCTCAGC(-5/-2)
BfrI 2401 CTTAAG C^TTAAG
BglI 40 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4206 AGATCT A^GATCT
BsgI 5098 CTGCAC GTGCAG(16/14)
BsiHKAI 1564 GAGCAC G[AT]GC[AT]^C
BsmBI 426 CGTCTC CGTCTC(1/5)
BspHI 4494 TCATGA T^CATGA
BspLU11I 1032 ACATGT A^CATGT
BsrBI 214 GAGCGG CCGCTC(-3/-3)
BsrDI 262 GCAATG GCAATG(2/0)
BssSI 4290 CTCGTG CACGAG(-5/-1)
BstUI 256 CGCG CG^CG
DraIII 1585 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
HpaI 2229 GTTAAC GTT^AAC
KspI 255 CCGCGG CCGC^GG
MfeI 2774 CAATTG C^AATTG
MunI 2774 CAATTG C^AATTG
MvnI 256 CGCG CG^CG
NaeI 279 GCCGGC GCC^GGC
NdeI 1855 CATATG CA^TATG
NgoMIV 279 GCCGGC G^CCGGC
PshAI 313 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5019 ATTAAT AT^TAAT
RcaI 4494 TCATGA T^CATGA
SacII 255 CCGCGG CCGC^GG
SgrAI 278 CGCCGGCG C[GA]^CCGG[CT]G
SwaI 609 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 3590 GTCTAC GT^[AC][GT]AC
4663 GTAGAC
AclI 2273 AACGTT AA^CGTT
4241 AACGTT
AlwNI 883 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
3035 CAGGGGCTG
AocI 3204 CCTTAGG CC^T[ACGT]AGG
4165 CCTGAGG
ApaI 302 GGGCCC GGGCC^C
3102 GGGCCC
Asp700I 2251 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4486 GAAAAGGTTC
BalI 3432 TGGCCA TGG^CCA
3948 TGGCCA
BcgI 4143 CGAGGCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2322 GCATCAAATTCG
Bsu36I 3204 CCTTAGG CC^T[ACGT]AGG
4165 CCTGAGG
CfoI 260 GCGC GCG^C
283 GCGC
EaeI 3432 TGGCCA [CT]^GGCC[GA]
3948 TGGCCA
Eco81I 3204 CCTTAGG CC^T[ACGT]AGG
4165 CCTGAGG
EcoRI 1864 GAATTC G^AATTC
4097 GAATTC
FauI 204 GCGGG CCCGC(4/6)
298 GCGGG
HhaI 260 GCGC GCG^C
283 GCGC
HinP1I 260 GCGC G^CGC
283 GCGC
HindIII 472 AAGCTT A^AGCTT
2082 AAGCTT
MluNI 3432 TGGCCA TGG^CCA
3948 TGGCCA
MscI 3432 TGGCCA TGG^CCA
3948 TGGCCA
MslI 372 CATCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
738 CATCCATGTG
NcoI 1185 CCATGG C^CATGG
3147 CCATGG
NspI 142 GCATGT [GA]CATG^[CT]
1032 ACATGT
PaeR7I 2168 CTCGAG C^TCGAG
2984 CTCGAG
Psp1406I 2273 AACGTT AA^CGTT
4241 AACGTT
PstI 1541 CTGCAG CTGCA^G
1973 CTGCAG
PvuII 961 CAGCTG CAG^CTG
4565 CAGCTG
ScaI 1690 AGTACT AGT^ACT
4613 AGTACT
SexAI 2526 ACCTGGT A^CC[AT]GGT
2619 ACCAGGT
SmaI 171 CCCGGG CCC^GGG
305 CCCGGG
XhoI 2168 CTCGAG C^TCGAG
2984 CTCGAG
XmaCI 171 CCCGGG C^CCGGG
305 CCCGGG
XmaI 171 CCCGGG C^CCGGG
305 CCCGGG
XmnI 2251 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4486 GAAAAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Cac8I CelII Cfr10I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI Hin1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII