##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbvCI BcgI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsgI BsiEI BsmBI BspEI
BsrBI BsrDI BssHII BstAPI BstEII
BstPI BstXI Bsu36I CelII CfoI
CpoI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccII 4280 CGCG CG^CG
AvaI 4871 CCCGAG C^[CT]CG[GA]G
BamHI 3148 GGATCC G^GATCC
BsaI 4375 GAGACC GGTCTC(1/5)
BsaWI 194 ACCGGA [AT]^CCGG[AT]
BseRI 2301 CTCCTC GAGGAG(10/8)
BsiWI 4276 CGTACG C^GTACG
BsmI 3747 GCATTC GAATGC(1/-1)
BsoBI 4871 CCCGAG C^[CT]CG[GA]G
BspDI 3656 ATCGAT AT^CGAT
BspLU11I 4333 ACATGT A^CATGT
BsrFI 1685 ACCGGC [GA]^CCGG[CT]
BssSI 4294 CACGAG CACGAG(-5/-1)
Bst1107I 5187 GTATAC GTA^TAC
BstUI 4280 CGCG CG^CG
BstZ17I 5187 GTATAC GTA^TAC
Cfr10I 1685 ACCGGC [GA]^CCGG[CT]
ClaI 3656 ATCGAT AT^CGAT
DraII 872 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1228 GACTACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 868 CCATGG C^C[GA][CT]GG
EcoO109I 872 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 3080 GATATC GAT^ATC
HindIII 3207 AAGCTT A^AGCTT
MvnI 4280 CGCG CG^CG
NcoI 868 CCATGG C^CATGG
PflMI 826 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 872 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 4590 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SnaBI 4274 TACGTA TAC^GTA
Van91I 826 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1032 CCAGGATTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 2025 CTTAAG C^TTAAG
5139 CTTAAG
AlwNI 1019 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1576 CAGTTCCTG
BanII 1559 GAGCTC G[GA]GC[CT]^C
3023 GAGCTC
BbrPI 1526 CACGTG CAC^GTG
1545 CACGTG
BciVI 415 GGATAC GTATCC(6/5)
2767 GTATCC
BclI 1838 TGATCA T^GATCA
2960 TGATCA
BcnI 284 CCCGG CC^[GC]GG
4906 CCCGG
BfrI 2025 CTTAAG C^TTAAG
5139 CTTAAG
BpmI 373 CTGGAG CTGGAG(16/14)
833 CTGGAG
BspHI 320 TCATGA T^CATGA
4942 TCATGA
BstYI 2002 GGATCT [GA]^GATC[CT]
3148 GGATCC
EcoT14I 868 CCATGG C^C[AT][AT]GG
3158 CCAAGG
FbaI 1838 TGATCA T^GATCA
2960 TGATCA
MflI 2002 GGATCT [GA]^GATC[CT]
3148 GGATCC
NciI 284 CCCGG CC^[GC]GG
4906 CCCGG
PleI 1123 GAGTC GAGTC(4/5)
3163 GAGTC
PmaCI 1526 CACGTG CAC^GTG
1545 CACGTG
PmlI 1526 CACGTG CAC^GTG
1545 CACGTG
RcaI 320 TCATGA T^CATGA
4942 TCATGA
SacI 1559 GAGCTC GAGCT^C
3023 GAGCTC
SapI 1168 GCTCTTC GCTCTTC(1/4)
1561 GCTCTTC
ScaI 2138 AGTACT AGT^ACT
5026 AGTACT
StyI 868 CCATGG C^C[AT][AT]GG
3158 CCAAGG
XhoII 2002 GGATCT [GA]^GATC[CT]
3148 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstBI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRI EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI NspV PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI