##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflIII
AgeI ApoI AscI AseI AsnI
Asp700I Asp718I BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EcoRI EcoT22I KpnI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI SfuI SgrAI SnaBI
Sse8387I SspBI SspI SwaI Tsp509I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1907 GACGTC GACGT^C
AccI 393 GTAGAC GT^[AC][GT]AC
AflII 3624 CTTAAG C^TTAAG
AhdI 1447 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2042 GGGCCC GGGCC^C
AspEI 1447 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4209 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2647 TGCGCA TGC^GCA
AvrII 500 CCTAGG C^CTAGG
BbeI 272 GGCGCC GGCGC^C
BbrPI 3854 CACGTG CAC^GTG
BbvCI 3956 GCTGAGG CCTCAGC(-5/-2)
BciVI 1833 GGATAC GTATCC(6/5)
BfrI 3624 CTTAAG C^TTAAG
BlnI 500 CCTAGG C^CTAGG
BsaBI 4712 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 993 GAGACC GGTCTC(1/5)
BsiEI 4459 CGGCCG CG[GA][CT]^CG
BsmBI 2874 GAGACG CGTCTC(1/5)
BstEII 2816 GGTCACC G^GT[ACGT]ACC
BstPI 2816 GGTCACC G^GT[ACGT]ACC
DrdI 2733 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4459 CGGCCG C^GGCCG
Eam1105I 1447 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4459 CGGCCG C^GGCCG
Eco52I 4459 CGGCCG C^GGCCG
EcoO65I 2816 GGTCACC G^GT[ACGT]ACC
EcoRV 3356 GATATC GAT^ATC
FspI 2647 TGCGCA TGC^GCA
KasI 272 GGCGCC G^GCGCC
KspI 1136 CCGCGG CCGC^GG
MamI 4712 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 272 GGCGCC GG^CGCC
PflFI 4209 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3854 CACGTG CAC^GTG
PmlI 3854 CACGTG CAC^GTG
SacII 1136 CCGCGG CCGC^GG
SexAI 1608 ACCAGGT A^CC[AT]GGT
SfiI 4891 GGCCAGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 272 GGCGCC GGC^GCC
SpeI 5021 ACTAGT A^CTAGT
StuI 4131 AGGCCT AGG^CCT
Tth111I 4209 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2268 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1137 CGCG CG^CG
3767 CGCG
AocI 3967 CCTGAGG CC^T[ACGT]AGG
4242 CCTGAGG
BcgI 421 CGATCCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2650 GCAAACTCTTCG
BclI 572 TGATCA T^GATCA
2768 TGATCA
BglII 3748 AGATCT A^GATCT
3991 AGATCT
BlpI 3591 GCTCAGC GC^T[ACGT]AGC
4857 GCTCAGC
Bpu1102I 3591 GCTCAGC GC^T[ACGT]AGC
4857 GCTCAGC
BsaWI 2719 ACCGGA [AT]^CCGG[AT]
4961 TCCGGT
BspHI 575 TCATGA T^CATGA
4085 TCATGA
BsrBI 1644 CCGCTC CCGCTC(-3/-3)
702 GAGCGG
BsrDI 955 GCAATG GCAATG(2/0)
3162 GCAATG
BstUI 1137 CGCG CG^CG
3767 CGCG
BstXI 1389 CCAGCGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2515 CCAGGCCCCTGG
Bsu36I 3967 CCTGAGG CC^T[ACGT]AGG
4242 CCTGAGG
CelII 3591 GCTCAGC GC^T[ACGT]AGC
4857 GCTCAGC
Eco81I 3967 CCTGAGG CC^T[ACGT]AGG
4242 CCTGAGG
EcoNI 623 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1935 CCTCCACGAGG
FbaI 572 TGATCA T^GATCA
2768 TGATCA
FseI 3813 GGCCGGCC GGCCGG^CC
4748 GGCCGGCC
HincII 346 GTTAAC GT[CT]^[GA]AC
2294 GTTAAC
HindII 346 GTTAAC GT[CT]^[GA]AC
2294 GTTAAC
HindIII 801 AAGCTT A^AGCTT
1776 AAGCTT
HpaI 346 GTTAAC GTT^AAC
2294 GTTAAC
MvnI 1137 CGCG CG^CG
3767 CGCG
NcoI 979 CCATGG C^CATGG
1111 CCATGG
RcaI 575 TCATGA T^CATGA
4085 TCATGA
SapI 1859 GAAGAGC GCTCTTC(1/4)
4568 GCTCTTC
ScaI 1081 AGTACT AGT^ACT
4073 AGTACT
XcmI 979 CCATGGTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2357 CCAAGGGCTTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI Aor51HI ApaLI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI