##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AflII AgeI Aor51HI AscI
AseI AsnI Asp700I BbeI BcgI
BclI BfrI BsaBI BsiWI BsmBI
BspHI BsrFI BssHII Bst1107I BstBI
BstZ17I Cfr10I CpoI Eco47III EcoRI
EcoRV FbaI FseI KasI KspI
MamI MluI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SpeI Sse8387I SspI StuI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 5123 GGTACC G^GTACC
AccI 2451 GTCTAC GT^[AC][GT]AC
AccII 4746 CGCG CG^CG
AccIII 4752 TCCGGA T^CCGGA
AhdI 1401 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1447 GTGCAC G^TGCAC
ApaLI 1447 GTGCAC G^TGCAC
Asp718I 5123 GGTACC G^GTACC
AspEI 1401 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 919 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2911 TGCGCA TGC^GCA
BamHI 5250 GGATCC G^GATCC
BbrPI 577 CACGTG CAC^GTG
BciVI 1985 GTATCC GTATCC(6/5)
BglI 1898 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4752 TCCGGA [AT]^CCGG[AT]
BseAI 4752 TCCGGA T^CCGGA
BspDI 4122 ATCGAT AT^CGAT
BspEI 4752 TCCGGA T^CCGGA
BssSI 4345 CACGAG CACGAG(-5/-1)
BstUI 4746 CGCG CG^CG
ClaI 4122 ATCGAT AT^CGAT
DrdI 1303 GACACACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3256 CGGCCG C^GGCCG
Eam1105I 1401 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3256 CGGCCG C^GGCCG
Eco52I 3256 CGGCCG C^GGCCG
FspI 2911 TGCGCA TGC^GCA
HindIII 4870 AAGCTT A^AGCTT
HpaI 1175 GTTAAC GTT^AAC
KpnI 5123 GGTACC GGTAC^C
MfeI 3890 CAATTG C^AATTG
MroI 4752 TCCGGA T^CCGGA
MunI 3890 CAATTG C^AATTG
MvnI 4746 CGCG CG^CG
PflFI 919 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 577 CACGTG CAC^GTG
PmlI 577 CACGTG CAC^GTG
PshAI 4804 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 4835 GCTCTTC GCTCTTC(1/4)
SexAI 4204 ACCAGGT A^CC[AT]GGT
SphI 1813 GCATGC GCATG^C
Tth111I 919 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1848 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 841 AACGTT AA^CGTT
4641 AACGTT
AcsI 1352 AAATTC [GA]^AATT[CT]
4661 AAATTT
ApoI 1352 AAATTC [GA]^AATT[CT]
4661 AAATTT
AvrII 885 CCTAGG C^CTAGG
5269 CCTAGG
BalI 2088 TGGCCA TGG^CCA
2815 TGGCCA
BlnI 885 CCTAGG C^CTAGG
5269 CCTAGG
BlpI 3051 GCTGAGC GC^T[ACGT]AGC
3821 GCTGAGC
Bpu1102I 3051 GCTGAGC GC^T[ACGT]AGC
3821 GCTGAGC
BsaI 1386 GAGACC GGTCTC(1/5)
2898 GGTCTC
BsiEI 2843 CGACCG CG[GA][CT]^CG
3256 CGGCCG
BsrBI 2534 GAGCGG CCGCTC(-3/-3)
3824 GAGCGG
BstEII 72 GGTGACC G^GT[ACGT]ACC
1360 GGTTACC
BstPI 72 GGTGACC G^GT[ACGT]ACC
1360 GGTTACC
CelII 3051 GCTGAGC GC^T[ACGT]AGC
3821 GCTGAGC
DraI 5090 TTTAAA TTT^AAA
5102 TTTAAA
EcoO65I 72 GGTGACC G^GT[ACGT]ACC
1360 GGTTACC
EcoT22I 991 ATGCAT ATGCA^T
1815 ATGCAT
HaeII 2488 AGCGCC [GA]GCGC^[CT]
4470 GGCGCT
HincII 1175 GTTAAC GT[CT]^[GA]AC
3609 GTCAAC
HindII 1175 GTTAAC GT[CT]^[GA]AC
3609 GTCAAC
MluNI 2088 TGGCCA TGG^CCA
2815 TGGCCA
MscI 2088 TGGCCA TGG^CCA
2815 TGGCCA
NdeI 537 CATATG CA^TATG
565 CATATG
NsiI 991 ATGCAT ATGCA^T
1815 ATGCAT
Psp1406I 841 AACGTT AA^CGTT
4641 AACGTT
SmaI 3151 CCCGGG CCC^GGG
4429 CCCGGG
SnaBI 834 TACGTA TAC^GTA
892 TACGTA
XmaCI 3151 CCCGGG C^CCGGG
4429 CCCGGG
XmaI 3151 CCCGGG C^CCGGG
4429 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII