################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AflII AgeI Aor51HI AscI AseI AsnI Asp700I BbeI BcgI BclI BfrI BsaBI BsiWI BsmBI BspHI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I CpoI Eco47III EcoRI EcoRV FbaI FseI KasI KspI MamI MluI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspI StuI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 5123 GGTACC G^GTACC AccI 2451 GTCTAC GT^[AC][GT]AC AccII 4746 CGCG CG^CG AccIII 4752 TCCGGA T^CCGGA AhdI 1401 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1447 GTGCAC G^TGCAC ApaLI 1447 GTGCAC G^TGCAC Asp718I 5123 GGTACC G^GTACC AspEI 1401 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 919 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2911 TGCGCA TGC^GCA BamHI 5250 GGATCC G^GATCC BbrPI 577 CACGTG CAC^GTG BciVI 1985 GTATCC GTATCC(6/5) BglI 1898 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 4752 TCCGGA [AT]^CCGG[AT] BseAI 4752 TCCGGA T^CCGGA BspDI 4122 ATCGAT AT^CGAT BspEI 4752 TCCGGA T^CCGGA BssSI 4345 CACGAG CACGAG(-5/-1) BstUI 4746 CGCG CG^CG ClaI 4122 ATCGAT AT^CGAT DrdI 1303 GACACACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3256 CGGCCG C^GGCCG Eam1105I 1401 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3256 CGGCCG C^GGCCG Eco52I 3256 CGGCCG C^GGCCG FspI 2911 TGCGCA TGC^GCA HindIII 4870 AAGCTT A^AGCTT HpaI 1175 GTTAAC GTT^AAC KpnI 5123 GGTACC GGTAC^C MfeI 3890 CAATTG C^AATTG MroI 4752 TCCGGA T^CCGGA MunI 3890 CAATTG C^AATTG MvnI 4746 CGCG CG^CG PflFI 919 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 577 CACGTG CAC^GTG PmlI 577 CACGTG CAC^GTG PshAI 4804 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 4835 GCTCTTC GCTCTTC(1/4) SexAI 4204 ACCAGGT A^CC[AT]GGT SphI 1813 GCATGC GCATG^C Tth111I 919 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1848 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AclI 841 AACGTT AA^CGTT 4641 AACGTT AcsI 1352 AAATTC [GA]^AATT[CT] 4661 AAATTT ApoI 1352 AAATTC [GA]^AATT[CT] 4661 AAATTT AvrII 885 CCTAGG C^CTAGG 5269 CCTAGG BalI 2088 TGGCCA TGG^CCA 2815 TGGCCA BlnI 885 CCTAGG C^CTAGG 5269 CCTAGG BlpI 3051 GCTGAGC GC^T[ACGT]AGC 3821 GCTGAGC Bpu1102I 3051 GCTGAGC GC^T[ACGT]AGC 3821 GCTGAGC BsaI 1386 GAGACC GGTCTC(1/5) 2898 GGTCTC BsiEI 2843 CGACCG CG[GA][CT]^CG 3256 CGGCCG BsrBI 2534 GAGCGG CCGCTC(-3/-3) 3824 GAGCGG BstEII 72 GGTGACC G^GT[ACGT]ACC 1360 GGTTACC BstPI 72 GGTGACC G^GT[ACGT]ACC 1360 GGTTACC CelII 3051 GCTGAGC GC^T[ACGT]AGC 3821 GCTGAGC DraI 5090 TTTAAA TTT^AAA 5102 TTTAAA EcoO65I 72 GGTGACC G^GT[ACGT]ACC 1360 GGTTACC EcoT22I 991 ATGCAT ATGCA^T 1815 ATGCAT HaeII 2488 AGCGCC [GA]GCGC^[CT] 4470 GGCGCT HincII 1175 GTTAAC GT[CT]^[GA]AC 3609 GTCAAC HindII 1175 GTTAAC GT[CT]^[GA]AC 3609 GTCAAC MluNI 2088 TGGCCA TGG^CCA 2815 TGGCCA MscI 2088 TGGCCA TGG^CCA 2815 TGGCCA NdeI 537 CATATG CA^TATG 565 CATATG NsiI 991 ATGCAT ATGCA^T 1815 ATGCAT Psp1406I 841 AACGTT AA^CGTT 4641 AACGTT SmaI 3151 CCCGGG CCC^GGG 4429 CCCGGG SnaBI 834 TACGTA TAC^GTA 892 TACGTA XmaCI 3151 CCCGGG C^CCGGG 4429 CCCGGG XmaI 3151 CCCGGG C^CCGGG 4429 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII