##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       AflII       AgeI        Aor51HI     AscI        
AseI        AsnI        Asp700I     BbeI        BcgI        
BclI        BfrI        BsaBI       BsiWI       BsmBI       
BspHI       BsrFI       BssHII      Bst1107I    BstBI       
BstZ17I     Cfr10I      CpoI        Eco47III    EcoRI       
EcoRV       FbaI        FseI        KasI        KspI        
MamI        MluI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SpeI        Sse8387I    SspI        StuI        SwaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       5123  GGTACC               G^GTACC
AccI         2451  GTCTAC               GT^[AC][GT]AC
AccII        4746  CGCG                 CG^CG
AccIII       4752  TCCGGA               T^CCGGA
AhdI         1401  GACACTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1447  GTGCAC               G^TGCAC
ApaLI        1447  GTGCAC               G^TGCAC
Asp718I      5123  GGTACC               G^GTACC
AspEI        1401  GACACTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          919  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2911  TGCGCA               TGC^GCA
BamHI        5250  GGATCC               G^GATCC
BbrPI         577  CACGTG               CAC^GTG
BciVI        1985  GTATCC               GTATCC(6/5)
BglI         1898  GCCACTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI        4752  TCCGGA               [AT]^CCGG[AT]
BseAI        4752  TCCGGA               T^CCGGA
BspDI        4122  ATCGAT               AT^CGAT
BspEI        4752  TCCGGA               T^CCGGA
BssSI        4345  CACGAG               CACGAG(-5/-1)
BstUI        4746  CGCG                 CG^CG
ClaI         4122  ATCGAT               AT^CGAT
DrdI         1303  GACACACTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3256  CGGCCG               C^GGCCG
Eam1105I     1401  GACACTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3256  CGGCCG               C^GGCCG
Eco52I       3256  CGGCCG               C^GGCCG
FspI         2911  TGCGCA               TGC^GCA
HindIII      4870  AAGCTT               A^AGCTT
HpaI         1175  GTTAAC               GTT^AAC
KpnI         5123  GGTACC               GGTAC^C
MfeI         3890  CAATTG               C^AATTG
MroI         4752  TCCGGA               T^CCGGA
MunI         3890  CAATTG               C^AATTG
MvnI         4746  CGCG                 CG^CG
PflFI         919  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         577  CACGTG               CAC^GTG
PmlI          577  CACGTG               CAC^GTG
PshAI        4804  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         4835  GCTCTTC              GCTCTTC(1/4)
SexAI        4204  ACCAGGT              A^CC[AT]GGT
SphI         1813  GCATGC               GCATG^C
Tth111I       919  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1848  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AclI          841  AACGTT               AA^CGTT
             4641  AACGTT              
AcsI         1352  AAATTC               [GA]^AATT[CT]
             4661  AAATTT              
ApoI         1352  AAATTC               [GA]^AATT[CT]
             4661  AAATTT              
AvrII         885  CCTAGG               C^CTAGG
             5269  CCTAGG              
BalI         2088  TGGCCA               TGG^CCA
             2815  TGGCCA              
BlnI          885  CCTAGG               C^CTAGG
             5269  CCTAGG              
BlpI         3051  GCTGAGC              GC^T[ACGT]AGC
             3821  GCTGAGC             
Bpu1102I     3051  GCTGAGC              GC^T[ACGT]AGC
             3821  GCTGAGC             
BsaI         1386  GAGACC               GGTCTC(1/5)
             2898  GGTCTC              
BsiEI        2843  CGACCG               CG[GA][CT]^CG
             3256  CGGCCG              
BsrBI        2534  GAGCGG               CCGCTC(-3/-3)
             3824  GAGCGG              
BstEII         72  GGTGACC              G^GT[ACGT]ACC
             1360  GGTTACC             
BstPI          72  GGTGACC              G^GT[ACGT]ACC
             1360  GGTTACC             
CelII        3051  GCTGAGC              GC^T[ACGT]AGC
             3821  GCTGAGC             
DraI         5090  TTTAAA               TTT^AAA
             5102  TTTAAA              
EcoO65I        72  GGTGACC              G^GT[ACGT]ACC
             1360  GGTTACC             
EcoT22I       991  ATGCAT               ATGCA^T
             1815  ATGCAT              
HaeII        2488  AGCGCC               [GA]GCGC^[CT]
             4470  GGCGCT              
HincII       1175  GTTAAC               GT[CT]^[GA]AC
             3609  GTCAAC              
HindII       1175  GTTAAC               GT[CT]^[GA]AC
             3609  GTCAAC              
MluNI        2088  TGGCCA               TGG^CCA
             2815  TGGCCA              
MscI         2088  TGGCCA               TGG^CCA
             2815  TGGCCA              
NdeI          537  CATATG               CA^TATG
              565  CATATG              
NsiI          991  ATGCAT               ATGCA^T
             1815  ATGCAT              
Psp1406I      841  AACGTT               AA^CGTT
             4641  AACGTT              
SmaI         3151  CCCGGG               CCC^GGG
             4429  CCCGGG              
SnaBI         834  TACGTA               TAC^GTA
              892  TACGTA              
XmaCI        3151  CCCGGG               C^CCGGG
             4429  CCCGGG              
XmaI         3151  CCCGGG               C^CCGGG
             4429  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspLU11I    BspMI       BsrDI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII