##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AscI Asp718I AvrII BbeI
BbrPI BcgI BlnI BsaHI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstUI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoRI EcoRV FseI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI PacI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 137 CTTAAG C^TTAAG
AgeI 3487 ACCGGT A^CCGGT
AocI 2510 CCTGAGG CC^T[ACGT]AGG
Aor51HI 905 AGCGCT AGC^GCT
ApaI 4811 GGGCCC GGGCC^C
AseI 146 ATTAAT AT^TAAT
AsnI 146 ATTAAT AT^TAAT
AspI 3715 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3558 TGCGCA TGC^GCA
BamHI 729 GGATCC G^GATCC
BanI 3490 GGTGCC G^G[CT][GA]CC
BclI 561 TGATCA T^GATCA
BfrI 137 CTTAAG C^TTAAG
BglI 303 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3147 AGATCT A^GATCT
BsiEI 1463 CGGTCG CG[GA][CT]^CG
Bsp1407I 2871 TGTACA T^GTACA
BsrGI 2871 TGTACA T^GTACA
BstAPI 2656 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 358 GGTGACC G^GT[ACGT]ACC
BstPI 358 GGTGACC G^GT[ACGT]ACC
Bsu36I 2510 CCTGAGG CC^T[ACGT]AGG
DraI 5047 TTTAAA TTT^AAA
Eco47III 905 AGCGCT AGC^GCT
Eco81I 2510 CCTGAGG CC^T[ACGT]AGG
EcoO65I 358 GGTGACC G^GT[ACGT]ACC
FbaI 561 TGATCA T^GATCA
FspI 3558 TGCGCA TGC^GCA
HaeII 905 AGCGCT [GA]GCGC^[CT]
HgaI 4965 GACGC GACGC(5/10)
HincII 3728 GTCAAC GT[CT]^[GA]AC
HindII 3728 GTCAAC GT[CT]^[GA]AC
NcoI 4900 CCATGG C^CATGG
NheI 4646 GCTAGC G^CTAGC
PaeR7I 4734 CTCGAG C^TCGAG
PflFI 3715 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3487 ACCGGT A^CCGGT
PshAI 4965 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 146 ATTAAT AT^TAAT
SapI 1534 GCTCTTC GCTCTTC(1/4)
SphI 4423 GCATGC GCATG^C
SspBI 2871 TGTACA T^GTACA
SwaI 5046 ATTTAAAT ATTT^AAAT
Tth111I 3715 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4734 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 656 GTCTAC GT^[AC][GT]AC
3572 GTAGAC
AhdI 4214 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4682 GACTTCCTGTC
Asp700I 286 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3856 GAAGGCCTTC
AspEI 4214 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4682 GACTTCCTGTC
BbvCI 1900 CCTCAGC CCTCAGC(-5/-2)
172 GCTGAGG
BsaAI 809 TACGTG [CT]AC^GT[GA]
1442 TACGTG
BsaBI 3142 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3964 GATTAAGATC
BspLU11I 3672 ACATGT A^CATGT
4698 ACATGT
BsrDI 1267 CATTGC GCAATG(2/0)
2713 CATTGC
BsrFI 3033 ACCGGC [GA]^CCGG[CT]
3487 ACCGGT
BssSI 857 CACGAG CACGAG(-5/-1)
3974 CACGAG
BstBI 1538 TTCGAA TT^CGAA
4088 TTCGAA
Cfr10I 3033 ACCGGC [GA]^CCGG[CT]
3487 ACCGGT
DrdI 1226 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3821 GACCCTGCTGTC
Eam1105I 4214 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4682 GACTTCCTGTC
EcoT22I 4166 ATGCAT ATGCA^T
4745 ATGCAT
HindIII 2272 AAGCTT A^AGCTT
2441 AAGCTT
MamI 3142 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3964 GATTAAGATC
NsiI 4166 ATGCAT ATGCA^T
4745 ATGCAT
NspV 1538 TTCGAA TT^CGAA
4088 TTCGAA
SacI 970 GAGCTC GAGCT^C
2519 GAGCTC
SfuI 1538 TTCGAA TT^CGAA
4088 TTCGAA
SmaI 1005 CCCGGG CCC^GGG
2470 CCCGGG
XbaI 1117 TCTAGA T^CTAGA
4382 TCTAGA
XcmI 363 CCATGAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4576 CCAAGTCTCAGATGG
XmaCI 1005 CCCGGG C^CCGGG
2470 CCCGGG
XmaI 1005 CCCGGG C^CCGGG
2470 CCCGGG
XmnI 286 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3856 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII