################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AscI Asp718I AvrII BbeI BbrPI BcgI BlnI BsaHI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstUI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV FseI Hin1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI PacI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflII 137 CTTAAG C^TTAAG AgeI 3487 ACCGGT A^CCGGT AocI 2510 CCTGAGG CC^T[ACGT]AGG Aor51HI 905 AGCGCT AGC^GCT ApaI 4811 GGGCCC GGGCC^C AseI 146 ATTAAT AT^TAAT AsnI 146 ATTAAT AT^TAAT AspI 3715 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3558 TGCGCA TGC^GCA BamHI 729 GGATCC G^GATCC BanI 3490 GGTGCC G^G[CT][GA]CC BclI 561 TGATCA T^GATCA BfrI 137 CTTAAG C^TTAAG BglI 303 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3147 AGATCT A^GATCT BsiEI 1463 CGGTCG CG[GA][CT]^CG Bsp1407I 2871 TGTACA T^GTACA BsrGI 2871 TGTACA T^GTACA BstAPI 2656 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 358 GGTGACC G^GT[ACGT]ACC BstPI 358 GGTGACC G^GT[ACGT]ACC Bsu36I 2510 CCTGAGG CC^T[ACGT]AGG DraI 5047 TTTAAA TTT^AAA Eco47III 905 AGCGCT AGC^GCT Eco81I 2510 CCTGAGG CC^T[ACGT]AGG EcoO65I 358 GGTGACC G^GT[ACGT]ACC FbaI 561 TGATCA T^GATCA FspI 3558 TGCGCA TGC^GCA HaeII 905 AGCGCT [GA]GCGC^[CT] HgaI 4965 GACGC GACGC(5/10) HincII 3728 GTCAAC GT[CT]^[GA]AC HindII 3728 GTCAAC GT[CT]^[GA]AC NcoI 4900 CCATGG C^CATGG NheI 4646 GCTAGC G^CTAGC PaeR7I 4734 CTCGAG C^TCGAG PflFI 3715 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3487 ACCGGT A^CCGGT PshAI 4965 GACGCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 146 ATTAAT AT^TAAT SapI 1534 GCTCTTC GCTCTTC(1/4) SphI 4423 GCATGC GCATG^C SspBI 2871 TGTACA T^GTACA SwaI 5046 ATTTAAAT ATTT^AAAT Tth111I 3715 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4734 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 656 GTCTAC GT^[AC][GT]AC 3572 GTAGAC AhdI 4214 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4682 GACTTCCTGTC Asp700I 286 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3856 GAAGGCCTTC AspEI 4214 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4682 GACTTCCTGTC BbvCI 1900 CCTCAGC CCTCAGC(-5/-2) 172 GCTGAGG BsaAI 809 TACGTG [CT]AC^GT[GA] 1442 TACGTG BsaBI 3142 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3964 GATTAAGATC BspLU11I 3672 ACATGT A^CATGT 4698 ACATGT BsrDI 1267 CATTGC GCAATG(2/0) 2713 CATTGC BsrFI 3033 ACCGGC [GA]^CCGG[CT] 3487 ACCGGT BssSI 857 CACGAG CACGAG(-5/-1) 3974 CACGAG BstBI 1538 TTCGAA TT^CGAA 4088 TTCGAA Cfr10I 3033 ACCGGC [GA]^CCGG[CT] 3487 ACCGGT DrdI 1226 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3821 GACCCTGCTGTC Eam1105I 4214 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4682 GACTTCCTGTC EcoT22I 4166 ATGCAT ATGCA^T 4745 ATGCAT HindIII 2272 AAGCTT A^AGCTT 2441 AAGCTT MamI 3142 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3964 GATTAAGATC NsiI 4166 ATGCAT ATGCA^T 4745 ATGCAT NspV 1538 TTCGAA TT^CGAA 4088 TTCGAA SacI 970 GAGCTC GAGCT^C 2519 GAGCTC SfuI 1538 TTCGAA TT^CGAA 4088 TTCGAA SmaI 1005 CCCGGG CCC^GGG 2470 CCCGGG XbaI 1117 TCTAGA T^CTAGA 4382 TCTAGA XcmI 363 CCATGAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4576 CCAAGTCTCAGATGG XmaCI 1005 CCCGGG C^CCGGG 2470 CCCGGG XmaI 1005 CCCGGG C^CCGGG 2470 CCCGGG XmnI 286 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3856 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII